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Molecular Organization and Biodynamics of the Photosystem II Reaction Center

Research Project

Project/Area Number 02454013
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionOkayama University

Principal Investigator

SATOH Kimiyuki  Okayama Univ., Faculty of Science, Professor, 理学部, 教授 (10032822)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Yuichiro  Okayama Univ., The Graduate School of Natural Science and Technology, Assistant, 大学院・自然科学研究科, 助手 (50183447)
山本 泰  岡山大学, 理学部, 助教授 (40091251)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1991: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1990: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsPhotosynthesis / Photosystem II / Reaction Center / D1 protein / Molecular Architecture / Charge Separation / Quinone / Protein Synthesis / 光化学系II反応中心 / D1蛋白質 / Pー680 / EPR / プロセシングプロテア-ゼ / 翻訳制御 / 光化学反応中心 / Cー末プロセシング / クロロフィル
Research Abstract

The efficient energy transformation in the primary process of photosynthesis is ensured by the highly ordered organization of photochemical reaction centers, in physical, chemical and biological sense. In photosystem II (PSII), the primary charge separation takes place in a pigmentprotein complex consisted of D1 and D2 proteins and some other components, which has recently been isolated and the chemical and physical properties have been analyzed (Nanba and Satoh, 1987). In the present study, the organization of PSII reaction center has been analyzed in two aspects. In the first part, stoichiometries of protein subunits and of pigments and redox cofactors of the isolated reaction center have been determined. The orientation of the primary donor (P-680) has been analyzed by EPR spectroscopy and it is deduced that the triplet state is localized on a chlorophyll, the tetrapyrrolic plane of which is tilted about 30゚ to the membrane. In the second part, the analysis has been focussed on some aspects of light-regulated turnover of D1 subunit of the PSII reaction center, which include the mechanism of light-regulated synthesis of D1 protein in isolated chloroplasts and the characterization of an enzyme involved in the C-terminal processing of D1 precursor protein. Determination of the levels of ATP in chloroplasts and the rate of synthesis of D1 protein under various conditions suggested that the level of ATP in soluble, stromal fraction controls the synthesis of D1 protein. The enzyme involved in the processing taking place after the synthesis has been extracted and purified.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] X.-S.Tang,K.Fushime and K.Satoh: "D1-D2 complex of the photosystem II reaction center from spinach:isolation and characterization." FEBS Lett.273. 257-260 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] E.J.E.van Mieghem,K.Satoh and A.W.Rutherford: "Chlorophyll tilted 30゚ relative to the membrane in the PSll reaction center." Biochim.Biophys.Acta.1058. 379-385 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kuroda,N.Inagaki and K.Satoh: "The level of stromal ATP regulates translation of the D1 protein in isolated chloroplasts." Plant & Cell Physiol.33. 33-39 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N.Inagaki and K.Satoh: "Accumulation of a translation intermediate of D1 protein by light-dark transition in isolated chloroplasts." FEBS Lett.300. 5-8 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Aizawa,T.Shimizu,T.Hiyama,K.Satoh,Y.Nakamura and Y.Fujita: "Changes in composition of membrane peptides in Synechocystis PCC6803 in response to light quality for photosynthetic growth." Photosynth.Res.32. 131-138 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Satoh: "Organization of the photosystem II reaction center." in “Regulation of Chloroplast Biogenesis"(J.II. Argyroudi-Akoyunoglou ed.). 367-372 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] X.-S. Tang, K. Fushime and K. Satoh: "D1-D2 complex of the photosystem II reaction center from spinach : Isolation and characterization" FEBS Lett. 273. 257-260 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] E.J.E. van Mieghem, K. Satoh and A.W. Rutherford: "Chlorophyll tilted 30゚ relative to the membrane in the PSII reaction center" Biochim. Biophys. Acta. 1058. 379-385 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kuroda, N. Inagaki and K. Satoh: "The level of stromal ATP regulates translation of the D1 protein in isolated chloroplasts" Plant & Cell Physiol. 33. 33-39 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Inagaki and K. Satoh: "Accumulation of a translation intermediate of D1 protein by light-dark transition in isolated chloroplasts" FEBS Lett. 300. 5-8 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Aizawa, T. Shimizu, T. Hiyama, K. Satoh, Y. Nakamura and Y. Fujita: "Changes in composition of membrane peptides in Synechocystis PCC 6803 in response to light quality for photosynthetic growth" Photosynth. Res. 32. 131-138 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Satoh: "Organization of the photosystem II reaction center" Regulation of Chloroplast Biogenesis (J. H. Argyroudi-Akoyunoglou ed.) Plenum Pub. Corp. NY. 367-372 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] E.J.E.van Mieghem,K.Satoh & A.W.Rutherford: "A chlorophyll tilted 30° rolative to the membrane in the Photosystem II reaction centre." Biochim.Biophys.Acta.1058. 379-385 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Nakane,M.Iwaki,K.Satoh & S.Itoh: "Artificial quinones replace the function of quinone electron acceptor (Q_A) in the isolated D1ーD2ーcytochrome b_<559> Photosystem II reaction center complex" Plant Cell Physiol.32(8). 1165-1171 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kuroda,N.Inagaki & K.Satoh: "The level of stromal ATP regulates translation of the D1 protein in isolated chloroplasts." Plant Cell Physiol.33(1). 33-39 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Inagaki & K.Satoh: "Accumulation of a translation intermediate of D1 protein by lightーdark transition in isolated spinach chloroplasts." FEBS Lett.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Satoh: "Organization of the photosystem II reaction center." Regulation of Chloroplast Biogenesis (J.H.ArgyroudiーAkoyunogloued.) Plenum Pub.Corp.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 公行: "光合成器官の分子構築と機能 現代植物生理学講座(分担)" 朝倉書店, 18 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Satoh: "Isolation and Properties of the Photosystem II Reaction Center,In J.Norris & J.Deisenhofer eds.“The Photosynthetic Reaction Center"" Academic Press, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Takahashi,K.Satoh and S.Itoh: "Silicomolybdate substitutes for the function of primary electron acceptor and stabilizes charge separtion in the photosystem II reaction center complex" FEBS Lett.255. 133-138 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Fujita,N.Inagaki,T.Ono,Y.Inoue and K.Satoh: "Cleavage of a synthetic COOHーterminal oligopeptide D1 precursor protein by a purified processing enzyme" FEBS Lett.255. 1-4 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Satoh,O.Hansson and P.Mathis: "Charge recombination between stabilized Pー680^+ and reduced cytochrome bー559 in quinoneーreconstituted PSII reaction center" Biochim.Biophys.Acta. 1016. 121-126 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Takahashi,H.Nakane,H.Kojima and K.Satoh: "Chromatographic purification and determination of the Carboxy terminal sequences of photosystem II reaction center Protein D1 and D2" Plant & Cell Physiol.31. 273-280 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Kobayashi,H.Maeda,T.Watanabe,H.Nakane and K.Satoh: "Chlorophyll a and βーcarotene content in the D_1/D_2/cytochrome bー559 reaction center complex from spinach" FEBS Lett.260. 138-140 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] X.ーS.Tang,K.Fushimi and K.Satch: "D1ーD2 complex of the photosystem II reaction center from spinach;Isolation and characterization" FEBS Lett.273. 257-260 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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