Nucleotide sequence of a fern phytochrome cDNA and antibody production

Research Project

Project/Area Number	02454014
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	植物生理学
Research Institution	Tokyo Metropolitan University
Principal Investigator	WADA Masamitsu Tokyo Metropol. Univ., Fac. Sci., Professor, 理学部, 教授 (60011681)
Project Period (FY)	1990 – 1991
Project Status	Completed (Fiscal Year 1991)
Budget Amount *help	¥6,800,000 (Direct Cost: ¥6,800,000) Fiscal Year 1991: ¥2,500,000 (Direct Cost: ¥2,500,000) Fiscal Year 1990: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywords	phytochorme / fern / anybody / cDNA / sequence / cloning
Research Abstract	The amino acid sequence of adiantum capillus-veneres photochrome has been deduced form the nucleuotide suquence of a cDNA clone (pFP1) which was initially identified by hybridization to a pea type I poytochrome cDAN clone. Ninety percent of nucleotide sequence has been done but ten percent still remained unidentified. Homology is high at the N-terminal region, especially around the chromophore attachment site, but lower at C-terminal region. Comparison among the amino acid sequences of type I, II, III of Arabidopsis and Adiantum phytochrome did not show any special resembrance of fern phytochrome to any of the three types of Arabidopsis. Overall, the fern phytochrome is rather similar to higher plant phytochrome as far as hydropathy profile is concerned, suggesting that the phytochrome molecule is not trans-membranous protein but water soluble, although fern phytochrome has been shown To raise anti-fern phytochrome antibodies, 3 polypeptides made of 15 amino acids around hydrophilic regions deduced from nucleuotide sequence of pFP1 were synthesized. Fusion protein of this clone was also produced as an antigen. Production of monoclonal antibodies of this fern phytochrome is now underway.