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Analysis of molecular mechanisms of cell division in Tetrahymena using cell-division-arrest mutants.

Research Project

Project/Area Number 02454020
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionUniversity of Tsukuba

Principal Investigator

WATANABE Yoshio  Univ. Tsukuba, Inst. Biol. Sci., Prof., 生物科学系, 教授 (00015918)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1992: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsTetrahymena / cell division / cytokinesis / cell-division mutant / actin / profilin / EF-1alpha / calcium-binding protein / 分裂停止突然変異株 / テトラヒメナ・アクチン / Ca^<2+>-結合蛋白質 / 分裂停止突然変異 / 収縮環 / DNA複製 / EFー1α / テトラヒメナ アクチン / テトラヒメナ プロフィリン / カルシウム 結合蛋白質 / アンチセンス RNA / 分裂溝
Research Abstract

To elucidate the molecular mechanisms of cell division in animal cells,by using Tetrahymena's cell-division-arrest mutants, cdaA and cdaC, we have analyzed mechanisms for division plane determination, division furrow formation, furrowing, contraction-related contractle ring microfilament disassembly, and for calcium-regulation of division furrow constriction.
Concerning division plane determination, it was evident that cdaA mutant gene product,p85, plaied a crucial role in the determination and it served as polymerization nuclei of the contractile ring actin filaments. As for division furrow constriction, by the analysis of cdaC mutant, the mutant gene product is considered to be an actin-binding protein capable of bundling the contractile ring microfilaments. We recently found a protein which associates with Tetrahymena's 14-nm filaments and we identified it as EF-1alpha from its gene analysis. We are now investigating the relation between EF-1alpha and cdaC gene product, since Tetrahymena EF-1alpha has an actin-bundling activity. In cytokinesis, it has been proposed that contractile ring microfilament disassembly occurs accompanying with the contraction of contractile ring. In this regard, we found that an actin-polymerization-inhibitory factor, profilin of Tetrahymena, colocalized with actin filaments in the division furrow, suggesting the involvement of profilin in the formation and disassembly of contractile ring microfilaments. Concerning the calcium-regulation of division furrow constriction, we cloned the genes for 3 kinds of calcium-binding proteins (calmodulin, TCBP-23, and TCBP-25) from Tetrahymena. We are now scrutinized their biological functions in cytokinesis by injecting the gene or modified gene products yielded in E. coli.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] Chba,H.,Hirono,M.,Edamatsu,M. & Watanabe,Y.: "Timing of formation of Tetrahymena contractile ring microfilaments investigated by inhibition with skeletal muscle actin" Develop. Genetics. 13. 210-215 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirono,M.,Suth,K.,Watanabe,Y. & Ohno,T.: "A Chimeric actin carrying N-terminal portion of Tetrahymena actin does not bind to DNase I" Biochem. Biophys. Res. Commun.184. 1151-1156 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirono,M.,Saijo-Kurita,K.,Kurashima,J.,Watanabe,Y. & Ohno,T.: "Expression of Tetrahymena actin in mammalian cells" Cell Biol. Int. Rep.16. 645-651 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takemasa,T.,TAkagi,T.,Edamatsu,M. & Watanabe,Y.: "Calmodulin cDANs two species of Tetrahymena" Biochim. Biophys. Acta. 1132. 219-221 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Edamatsu,M.,Hirono,M. & Watanabe,Y.: "Tetrahymena profilin is localized in the division furrow" J.Biochem.112. 111-116 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kurasawa,Y.,Numata,O.,Kato,M.,Hirano,H.,Chiba,J. & Watanabe,Y.: "Identification of Tetrahymena 14-nm filament-associated protein as elongation factor 1α" Exptl. CEll Res.203. 251-258 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ohba,M.,Hirono,M., Takemasa,T. & Watanabe,Y.: "Timing of formation of Tetrahymena contractile microfilaments investigated by inhibition with skeletal muscle actin" Developmental Genetics. 13. 210-215 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirono,M.Sutoh,K.,Watanabe,Y. & Ohno,T.: "A chimeric actin carrying N-terminal portion of Tetrahymena actin does not bind to DNase I" Biochem. Biophys. Res. Commun.184. 1511-1516 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirono,M.,Saijo-Kurita,K.,Kurashima,J.,Watanabe,Y. & Ohno,T.: "Expression of Tetrahymena actin in mammalian cells" Cell Biol. Int. Rep.16. 645-651 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takemasa,T.,Takagi,T.,Edamatsu,M. & Watanabe,Y.: "Calmodulin cDNAs from two species of Tetrahymena" Biochim. Biophys. Acta. 1132. 219-221 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Edamatsu,M.,Hirnon,M. & Watanabe,Y.: "Tetrahymena profilin is localized in the division furrow" J. Biochem.112. 111-116 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kurasawa,Y.,Numata,O.,Katoh,M.,Hirano,H.,Chiba,J. & Watanabe,Y.: "Identification of Tetrahymena 14-nm filament-associated protein as elongation factor 1alpha" Exptl. Cell Res.203. 251-258 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Edamatsu,M.,Hirono,M.,Takemasa,T. & Watanabe,Y.: "The primary structure of Tetrahymena profilin" Biochem. Biophys. Res. Commun.175. 543-550 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Numata,O.,Tomiyoshi,T.,Kurasawa,Y.,Fu,Z-x., Takahashi,M.,Kosaka,T.,Chiba,J. & Watanabe,Y.: "Antibodies against Tetrahymena 14-nm filament-forming protein recognize the replication band in Euplotes" Exptl. Cell Res.193. 183-189 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Numata,O.,Takemasa,T.,Takagi,I.,Hirono,M.,Hirano,H.,Chiba,J. & Watanabe,Y.: "Tetrahymena 14-nm filament-forming protein has citrate synthase activity" Biochem. Biophys. Res. Commun.174. 1028-1034 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kimura,M.,Hirono,M.,Takemasa,T. & Watanabe,Y.: "Drastic change in the level of actin mRNA in the course of synchronous division in Tetrahymena" J. Biochem.109. 399-403 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takagi,I.,Numata,O. & Watanabe,Y.: "Involvement of 14-nm filament-forming protein and tubulin in gametic pronuclear behavior during conjugation in Tetrahymena" J. Protozool.38. 345-351 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takemasa,T., Takagi, T. & Watanabe,Y.: "Calcium-binding proteins from Tetrahymena" In Novel Calcium-Binding Proteins, ed. Heizman,Springer Press. 481-495 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Edamatsu,M.,Hirono,M. & Watanabe,Y.: "Purification and characterization of Tetrahymena profilin" Biochem. Biophys. Res. Commun.170. 957-962 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Watanabe,Y.,Ohba,H.,Hirono,M. & Yasuda,T.: "Analysis of furrow formation and furrowing during cell division in Tetrahymena using cell-division-arrest mutants" Ann. New York Acad. Sci.582. 166-178 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Watanabe,Y.,Hirono,M.,Takemasa,T. & Numata,O.: "Protozoan genes: As viewed from five Tetrahymena genes" Japan. J. Protozool.23. 1-11 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Watanabe,Y.,Hirano-Ohnishi,J. & Takemasa,T.: "Calcium-binding proteins and ciliary movement regulation in Tetrahymena" In Calcium as an Intracellular Messenger in Eucaryotic Microbes, ed. O'Day,Amer. Soc. Microbiol., Washington. 346-361 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takemasa,T.,Takagi,T.,Kobayashi,T.,Konishi,K. & Watanabe,Y.: "The third calmodulin family protein in Tetrahymena. Cloning of the cDNA for Tetrahymena calcium-binding protein of 23KDa" J. Biol. Chem.265. 2514-2517 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hirono,M.,Tanaka,R. & Watanabe,Y.: "Tetrahymena actin: Copolymerization with skeletal muscle actin and interactions with actin-binding proteins" J. Biochem.107. 32-36 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ohba,H.,Hirono,M.Edamatsu,M & Watanabe,Y.: "Timing of formation of Tetrahymena contractile microfilaments investigated by inhibition with skeletal muscle actin" Developmental Genetics. 13. 210-215 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirono,M.Sutoh,K.Watanabe,Y. & Ohno,T.: "A chimeric actin carrying N-terminal protein of Tetrahymena actin does not bind to DNase I" Biochemical and Biophysical Res.Commun.184. 1151-1156 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hirono,M.,Saijo-Kurita,K.,Kurashi-ma,J.& Watanabe,Y.: "Expression of Tetrahymena actin in mammalian cells" Cell Biology International Report. 16. 645-651 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takemasa,T.,Takagi,T,Edamatsu,M.& Watanabe,Y.: "Calmodulin cDNAs from two species of Tetrahymena" Biochim.Biophys.Acta. 1132. 219-221 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Edamatsu,M.,Hirono,M.& Watanabe,Y.: "Tetrahymena profilin is localized in the division furrow" J.Biochem.112. 111-116 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kurasawa, Y.,Numata.O,Hirono,M.& Watanabe,Y.: "Identification of Tetrahymena 14-nm filament-associated protein as elongation factor 1α" Exptl.Cell Res.203. 251-258 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ohba,H.,Hirono,M.,Edamatsu,M.and Watanabe,Y.: "Timing of formation of Tetrahymena contractile ring microfilaments investigated by inhibition with skeletal muscle actin." Developmental Genetics. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kimura,M.,Hirono,M.,Takemase,T.and Watanabe,Y.: "Drastic change in the level of actin mRNA in the course of synchronous division in Tetrahymena." Journal of Biochemistry. 109. 399-403 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Edamatsu,M.,Hirono,M.and Watanabe,Y.: "The primary structure of Tetrahymena profilin." Biochem.Biophys.Res.Commun.175. 543-550 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Numata,O.,Takagi,I.,Fuーchan,J.,Takahashi,M.,Kosaka,T.,and Watanabe,Y.: "Antibodies against Tetrahymena 14ーmm filamentーforming protein recognize the replication land in Euplotes." Exptal.Cell Res.193. 183-189 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Numata,O.,Takagi,I.,Hirano,M.,Takemase,T.and Watanabe,Y.: "Tetrahymena 14ーmm filamentーforming protein has citrate synthase activity." Biochem.Biophys.Res.Commun.175. 1028-1034 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takagi,I.,Numata,O.,Sugai,T.and Watanabe,Y.: "Involvement of 14ーmm filament forming protein and tubulin in gametic pronuclear behavior during conjugation in Tetrahymena." Journal of Protozoology. 38. 345-351 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takemasa,T.,Takagi,N.and Watanabe,Y.: "Calciumーbinding proteins from Tetrahymena.In Novel CalciumーBinding Proteins.Fundamentals and Clinical Implications.,ed.by C.W.Heizman,Chapter26" Springer Press, 481-495 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Watanabe,Y.,Ohba,H.,Hirono,M.,& Yasuda,T.: "Analysis of furrow formation and furrowing during cell division in <Tetrahymona>___ー using cellーdivisionーarrest mutants." Ann.New York Acad.Sci.582. 166-178 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.,Hirono,M.,Takemasa,T.,& Numata,O: "Protozoan genes:As viewed from five <Tetrahymena>___ー genes." Japan.J.Protozool.23. 1-11 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirono,M.,Tanaka,R.,& Watanabe,Y.: "<Tetrahymena>___ー actin:Copolymerization with skeletal muscle actin and interactions with muscle actinーbinding proteins." J.Biochem.107. 32-36 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takemasa,T.,Kobayashi,T.,Takagi,T.,Konishi,K.,& Watanabe,Y.: "The third calmodulin family protein from <Tetrahymena>___ー:Cloning and sequencing of gene for <Tetrahymena>___ー calciumーbinding protein of 23kda (TCBPー23)." J.Biol.Chem.265. 2514-2517 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Edamatsu,M.,Hirono,M.,& Watanabe,Y.: "Purification and properties of <Tetrahymena>___ー profilin." Biochem.Biophys.Res.Commun.170. 957-962 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kimura,M.,Hirono,M.,Takemasa,T.,& Watanabe,Y.: "Drastic change in the level of actin mRNA in the process of synchronous division in <Tetrahymena>___ー" J.Biochem.108. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.,HiranoーOhnishi,J.,& Takemasa,T.: "Calciumーbinding proteins and ciliary movement regulation in <Tetrahymena>___ー Chaptor 18,In Calcium as a Second Messenger in Eucaryotic Microbes,ed.by D.H.O'Day.,P.343ー361." American Society of Microbiology, 418 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takemasa,T.,Takagi,T.,& Watanabe,Y.: "Calciumーbinding protein in <Tetrahymena>___ー.In Novel CalciumーBinding ProteinsーFundamentals and Clinical Implications.ed.by C.W.Heizmann." Springer,Heidelberg, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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