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Study on the nature of amphibian cellinteractions by novel technology

Research Project

Project/Area Number 02454025
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionKyushu University

Principal Investigator

YAMANA Kiyotaka  Kyusyu Univ., Fac. of Sci., Professor, 理学部, 教授 (20037162)

Co-Investigator(Kenkyū-buntansha) NOMURA Kazuya  Kyusyu Univ., Fac. of Sci., Assoc. Professor, 理学部, 助教授 (30150395)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsCadherin / Xenopus laevis / Newt / Glycolipid / Lactone / Frog / Glycoprotein / Substratum adhesion molecules / ガングリオシド / イモリ / 細胞接着複合体
Research Abstract

What kind of molecules and what kind of interactions are involved in cell adhesion of developing frog embryos? By reverse PCR technique using cadherin consensus sequence primers, we isolated three new frog (Xenopus laevis) cadherin cDNAs and two newt (Cynops pyrrhogaster) cadhrin cDNAs. The entire base sequence of one of the frog cDNA clones was determined. The sequence had some similarity to frog EP-orXB-cadherin, but the sequence difference was apparent. At least five diffrent cadherins were expressed on one frog cell simultaneously. This observeation led us to a hypothesis that the cell adhension is mediated by supramolecular cadherin complexes present on cell surfaces. The DNA sequence was successfully used to design antisense oligonucleotides for antisense knocking out experiments of maternal cadherin mRNAs. By using time resolved streak-camera based fluorometry, we showed that one specific carbohydrate is associated with cadherins on cell surface. Several glycoproteins and glycol … More ipids had the carbohydrates, and this is the first identification of cadherin associated carbohydrate, the expression of which is regulated cell-cycle dependently. The possibility that these carbohydrates are on molecular chaperones for cadherin in being examined in our laboratory. The role of this carbohydrate in cell adhesion is apparent since the monoclinal antibody recognizing this carbohydrate (M4B raised by Prof. C. C. Wylie's group) disrupts Ca^<2+> dependent adhesion of frog blastula cells. We determined the structure of this epitope on neutral glycolipids from frog embryonic or somatic cells. The structure was entirely new, and we are now studying the receptor molecules for the carbohydrates participating Ca^<2+> dependent cell-cell interaction. Eptiope mapping of the monoclinal antibody FAD-II disrupting frog cell-substratum adhesion was also done, and the epitope was also on a neutral glycolipid. The possible roles of neutral glycolipids in cell adhesion are being examined in our laboratory. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Kazuya Nomura et al.: "A novel monoclonal antibody disrupting cell type specific substratum adhesion of frog (Xenopus laevis)epithelial cells and endothelial" Development Growth&Differentiation. 33(6). 639-649 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuya Nomura: "A novel monoclinal antibody disrupting cell type specific substratum adhesion of frog (Xenopus laevis) epithelial cells and endothelial cells" Develop. Growth & Differ.33(6). 639-649 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuya Nomura: "Cell adhesion and Morphogenesis" Seitainokagaku. 41(2). 84-87 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuya Nomura et al.: "A novel monoclonal antibody disrupting cell type specific substratum adhesion of frog(Xenopus laevis)epithelial cells and endothelial cells" Development Growth&Differentiation. 33(6). 639-649 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野村 一也: "細胞接着と形態形成" 生体の科学. 41. 84-87 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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