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The role of auxin-binding protein during growth and development of fruit

Research Project

Project/Area Number 02454046
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 園芸・造園学
Research InstitutionNagoya University

Principal Investigator

YAMAKI Shohei  Nagoya University, Professor School of Agriculture, 農学部, 教授 (70210341)

Co-Investigator(Kenkyū-buntansha) OHNO Hajime  Nagoya University, Agriculture Assistant Professor, 農学部, 助手 (20126840)
TEZUKA Takafumi  Nagoya University, Agriculture Associate Professor, 農学部, 助教授 (10109316)
SAKAI Shingo  Tukuba University, Biological Science Associate Professor, 生物科学系, 助教授 (60033388)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1991: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1990: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsAuxin / IAA / Auxin-binding protein / Protoplast / Melon / Apple / Cymbidium / プロトプラスト
Research Abstract

Auxin-binding protein in apple seedling was partially purified by 2, 4-D linked-Sephasrose 4B affinity chromatography and 2 times of Sepharose 4B gel filtration. It had a molecular weight of about 400 KDa and was composed of subunit bands of 56 KDa ana 15 KDa by SDS-PAGE, which appeared in proportion to the strength of 6binding activity. The dissociation constant by Scatchard plot was 7.7X1O^<-6> M. This binding protT4n is similar to ABP-I from mung bean seedling. The binding activity to ^<14>C-IAA was completely inhibited by 2, 4-D and NAA, and inhibited to about 50% by PCIB of antiauxin. Auxin-binding protein in melon seedling partially purified by the same procedure showed the equal activity between both assay methods of an ammonium sulfate precipitation and an equilibrating dialysis. The protein had a molecular weight of 155 KDa and a dissociation constant of 4.8X1O^<-6> M, and combined more than one molar IAA to one molar protein. This binding protein from mellon seedling is like ABP-II from mung bean seedling.
The function about two kinds of the auxin-binding protein was not investigated in detail through the present experiment. However, we can suggest firmly these auxin binding proteins possess the characteristics as an auxin receptor, as defined by the specificity for substrate, the saturation of reaction to substrate concentration and the reversibility of reaction.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Soejima,J.,Watanabe,M.,Moriguchi,T.and Yamaki,S.: "Good correlation between enzyme-linked immunosorbent assay and gas chromatographic analysis of abscisic acid in apple organs" J.Japan.Soc.Hort.Sci.58. 819-826 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamaki,S.and Asakura,T.: "Stimulation of the uptake of sorbitol into vacuoles from apple fruit flesh by abscisic acid and into protoplast by indoleacetic acid" Plant Cell Physiol.32. 315-318 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Murakami-Mizukami,Y.,Yamamoto,Y.and yamaki,S.: "Analyses of indole acetic acid and abscisic acid contents in nodules of soybean plants bearing VA mycorrhizas" Soil Sci.Plant Nutr.37. 291-298 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohno,H.and KaKo,S.: "Roles of floral organs and phytohormones in flower stalk elongation of Cymbidium (Orchidaceae)" J.Japan.Soc.Hort.Sci. 60. 159-165 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai,S.: "Reguratory functions of soluble auxin-binding proteins" International Review of Cytology. 135. 765-773 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai,S.: "Purification of a soluble cytokinin-binding protein from etiolated mung bean seedlings." Biosci.Biotech.Biochem.56. 504-507 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 酒井 慎吾: "現代植物生理学3 生長と分化" 朝倉書店, 24 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Soejima, J., Watanabe, M., Moriguchi, T. and Yamaki, S.: "Good correlation between enzyme-linked immunosorbent assay and gas chromatographic analysis of abscisic acid in apple organs." J. Japan. Soc. Hort. Sci.58. 819-826 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamaki, S. and Asakura, T.: "Stimulation of the uptake of sorbitol into vacuoles from apple fruit flesh by abscisic acid and into protoplast by indoleacetic acid." Plant Cell Physiol.32. 315-318 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Murakami-Mizukami, Y., Yamamoto, Y. and Yamaki, S.: "Analyses of indol acetic acid and abscisic acid contents in nodules of soybean plants bearing VA mycorrhizas." Soil Sci. Plant Nutr.37. 291-298 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohno, H. and Kako, S.: "Roles of floral organs and phytohormones in flower stalk elongation of Cymbidium (Orchidaceae)." J. Japan. Soc. Hort. Sci.60. 159-165 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai, S.: "Regulatory functions of soluble auxin-binding proteins." International Review of Cytology.135. 765-773 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai, S.: "Purification of a soluble cytokinin-binding protein from etiolated mung bean seedlings." Biosci. Biotech. Biochem.56. 504-507 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamaki,S.and Asakura,T.: "Stimulation of the uptake of sorbitol into vacuoles from apple fruit flesh by abscisic acid and into protoplast by indoleacetic acid" Plant Cell Physiology. 32. 315-318 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] MurakamiーMizukami,Y.Yamamoto,Y.and Yamaki,S.: "Analyses of indole acetic acid and abscisic acid contents in nodules of soybean plants bearing VA mycorrhizas" Soil Sci.Plant Nutr.37. 291-298 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ohno,H.and Kako,S.: "Roles of floral organs and phytohormones in flower stalk elongation of Cymbidium (Orchidaceae)" J.Japan.Soc.Hort.Sci.60. 159-165 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakai,S.: "Reguratory functions of soluble auxinーbinding proteins" International Review of Cytology. 135. 765-773 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakai,S.: "Purification of a soluble cytokininーbinding protein from etiolated mung bean seedlings" Biosci,Bioteck,Biochem. 56. 504-507 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Yamaki and T.Asakura: "Stimulation of the uptake of sorbitol into vacuoles from apple fruit flesh by abscisic acid and into protoplasts by indoleacetic acid" Plant and Cell Physiol.32. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Ohno and S.Kako: "Roles of floral organs and phytohormones in flower stalk elongation of Cymbidium (Orchidaceal)" J.Japan.Soc.Hort.Sci.60. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 酒井 慎吾: "現代植物生理学3 生長と分化" 朝倉書店, 24 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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