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Signal transduction in induction of bio-defense and pathogen-reception in plants infected with fangi

Research Project

Project/Area Number 02454051
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物保護
Research InstitutionNagoya University

Principal Investigator

DOKE Noriyuki  Nagoya University School of Agricultural Sciences Professor, 農学部, 教授 (80023472)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsplant pathogenic fungus / plant bio-defense / siganal transduction / infection response / elicitor / pathogenicity determinants / suppressor / superoxide generation / Phytophthora属菌 / エリシタ- / サプレッサ- / Alternaria属菌 / 情報伝達機構 / 過敏感細胞反応の誘導 / 宿主選択的毒素の作用機構 / <Phytohythora>___ー属菌 / <Biporalis>___ー <zeicola>___ー / <Alternaria>___ー <solani>___ー / 情報伝達因子 / 感染誘導 / グルカンサプレッサ- / O_2^-の生成反応
Research Abstract

Signal transduction in response of plant cells to fungal infection and its elicitor and regulatory aspects of the signal transduction by host-selective pathogenicity-determinants in relation to pathogen-reception were determined and discussed. Potato tuber tissues hypersensitively reacted to infection with an incompatible race of Phytophthora infestans and its hyphal wall components (HWC) with a transient enhancement of a superoxide-generating system (SOX), phospholipase A_2, lipoxygenase and lipid-peroxidation in association with following production of phytoalexin. G-protein, Ca^<++>, calmodulin (CaM), Ca^<++>/Cam dependent protein kinase were shown to be involved in the activation of SOX by using inhibitors. The in vitro activation of SOX in plasmamembrane fraction of potato tissues was stimulated by HWC-treatment in the presence of Ca^<++>, ATP and soluble protein fraction. Suspension cultured cells of several Slanaceous plants responsed to HWC, showing an immediate movement of Ca^ … More <++> from membrane and activation of superoxide generation l min later. Ca^<++> chelator and channel-blocker suppressed the occurrence of these HWC-stimulated phenomena in the cells. A water soluble glucan (WSG) isoalted from Phytophthora mycelia or germination fluid also suppressed the occurrence of HWC-stimulated Ca^<++> relaese and SOX-activation in susceptible, but not resistant, host cells. The role of WSG was enphasized as a host-selective factor to suppress the signal transduction for the active response of plant cells to elicitor or infection. Modes of action of host-selective toxins, AF-and AL-toxins from Alternaria alternata strawberry and tomato pathotypes, respectively, were investigated. Af-toxin acted on a putative receptor on plasamamembrane of the susceptible clones, inducing pathogen-reception. AL-toxin was proposed to acte against phosphoethanolamine transferase of the susceptible tomato cultivas, causing a membrane dissociation by a inhibition of phosphatidylethanolamine synthesis. A host selective toxin isolated Bipolaris zeicola race 3, BZR toxin was characterized by 4 co-toxins which were synergistically active as susceptibility-inducer in the fungal hosts. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] Kawaguchi: "Changes in amino acid and amine contents of susceptible tomato cultivars infected with Alternaria alternata tomato pathotype or treated with AL-toxin" Ann.Phytopath.Soc.Japan. 57. 526-533 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee,S.-S.: "Effect of host specific AF-toxins from Alternaria alternata strawberry pathotype of on viability of strawberry cultured cells." Ann.Phytopath.Soc.Japan. 57. 671-679 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 道家 紀志: "植物ー病原糸状菌相互作用の分子機構" 植物防疫. 45. 19-25 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee,S.-S.: "Protective effect of homologues of host-specific Af-toxin I produced by Alternaria alternata strawberry pathotype on strawberry cells." Physiol.Mol.Plant Pathol.40. 177-186 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao,J.-Z.: "Further evaluation of the significance of BZR-Toxin produced by Bipolaris zeicola race 3 in pathogenesis on rice and maize plants." Physiol.Mol.Plant Pathol.40. 359-370 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza,N.P.: "Effeect of AL-toxin produced by Alternaraia alternata tomato patho-type on cultured roots of tomato" Ann.Phytopath.Soc.Japan. 58. 411-415 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza,N.P.: "Inhibittory effect of AL-toxin produced by Akternaria alternata tomato pathotype on the biosynthesis of phosphatidylethanolamine in tomato leaves susceptible to the fungus" Ann.Phytopath.Soc.Japan. 58. 719-725 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sanchez,L.M.: "Host selective suppression by water-soluble glucans from Phytophtoraspp.on hypersensitive cell death of suspension-cultured cells from some solanaceous plants caused by hyphal wall elicitors of the fungi" Ann.Phytopath,soc.Japan. 58. 664-670 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 川北 一人: "植物の感染応答におけるシグナル伝達" 植物細胞工学. 14. 253-260 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 道家 紀志: "植物病原糸状菌レースと宿主品種間の寄生特異性の決定機構" 植物細胞工学. 15. 19-27 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sanchez,L.M.: "Involvement of suppressor-glucans and plant epidermal cells in host selective pathogenesis of Phytophthor capsici" J.Phytopathol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sanchez,L.M.: "Elicitor-induced chemiluminescence in cell suspension cultures of tomato,sweet pepper and tomato plants and its inhibition by suppressors from Phytophthora spp." Plant Science. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee,S.-S.: "Stimulation of phospholypase A_2 activity in strawberry cells treated with AF-toxins produced by Alternaria slternata strawberry pathotype" Physiol.Mol.Plant Pathol.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 道家 紀志: "Phytophthora属菌の水容性グルカンの病理的機能 久能均編 生物相互の認識機構" 日本植物病理学会, 145 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] DoKe,N.: "Involvement of active oxygen in induction of palnt defence response against infection and injury.In:E.Pell & K.Stefen,eds,Active Oxygen/Oxydative Stress and Matabolism." American Soc.Plant Physiol., 286 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 道家 紀志: "過敏感反応と情報伝達.奥八郎ら編 植物感染生理学最近の進歩" 植物感染生理最近の進歩刊行会, 233 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke,N.: "Involvement of superoxide in signal transduction:Response to attack by pathogens,physical and chemical shocks and U.V.irradiation.In:C.H.Foyer & P.M.Mulineaux,eds,Photooxidative Stress on Plants:" CRC Press, (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawaguchi: "Changes in amino acid and amine contents of susceptible tomato cultivars infected with Alternaria alternata tomato pathotype or treated with AL-toxin" Ann. Phytopath. Soc. Japan. 57. 526-533 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee. S.-S: "Effect of host specific AF-toxins from Alternaria alternata strawberry pathotype of on viability of strawberry cultured cells." Ann. Phytopath. Soc. Japan. 57. 671-679 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke, Noriyuki: "Molecular mechanisms of plant host-pathogenic fungus interaction." Shokubutsu boeki. 45. 19-25 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee. S.-S.: "Protective effect of homologues of host-specific AF-toxin I produced by Alternaria alternata strawberry pathotype on strawberry cells." Physiol. Mol. Plant Pathol. 135. 177-186 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Xiao, J.-Z.: "Further evaluation of the significance of BZR-toxin produced by Bipolaris zeicola race 3 in pathogenesis on rice and maize plants" Physiol. Mol. Plant Pathol. 40. 359-370 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza, N. P.: "Effect of AL-toxin produced by Alternaria alternata tomato pathotype on cultured roots of tomato" Ann. Phytopath. Soc. Japan. 58. 411-415 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza, N.: "Inhibittory effect of AL-toxin produced by Alternaria alternata tomato pathotype on the biosynthesis of phosphatidyl-ethanolamine in tomato leaves suceptible to the fungus." Ann. Phytopath. Soc. Japan. 58. 719-725 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sanchez, L.M.: "Host selective suppression by water-soluble glucans from Phytophthora spp. on hypersensitive cell death of suspension- cultured cells from some solanaceous plants caused by hyphal wall elicitors of the fungi." Ann. Phytopath. soc. Japan. 58. 664-670 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawakita, Kazuto: "Signal transduction in elicitation of defense reaction in plants." Plant Cell Thechnology. 4. 253-260 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke, Noriyuki: "Race-cultivar specificity in plant fungal disease and its molecular mechanisms" Plant Cell Technology. 15. 19-27 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sanchez, L.M.: "Involvement of suppressor-glucans and plant epidermal cells in host selective pathogenesis of Phytophthora capsici." J.Phytopathol. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sanchez, L.M.: "Elicitor-induced chemiluminescence in cell suspension- cultures of tomato, sweet pepper and tomato plants and its inhibition by suppressors from Phytophthora spp" Plant Science. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Lee, S.-S.: "Stimulation of phospholypase A^2 activity in strawberry cells treated with AF-toxins produced by Alternaria alternata strawberry pathotype" Physiol. Mol. Plant Pathol. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke, Noriyuki: "water solubule beta-glucans of Phytophthora spp.: their pathological function." The dynamics of Recognition in interactions of Organisms, Ed, H. Kunoh. 1-22 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke, N.: "Involvement of active oxygen in induction of palnt defense response against infection and injury." E.Pell & K.Stefen, eds, Active Oxygen/Oxydative Stress and Metabolism. 84-96 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke,Noriyuki: "Hypersensitive reaction and itssignificance in signal transduction for plant active defense" Recent Advance in Physiological Plant Pathology, Eds, H. Oku et al.84-96 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Doke,N.: "Involvement of superoxide in signal transduction: Response to attack by pathogens, physical and chemical shocks and U.V. irradiation" C.H.Foyer & P.M.Mulineaux,eds,Photooxidative Stress on Plants:Causes and Amelioration. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Orolaza,N.: "Inhibitory effect of AL-toxin produced by Alternaria alternata tomato pathotype on the biosynthesis of phosphatidylethanolamine in tomato leaves susceptible to the fungus." Ann.Phytopathol.Soc.Japan. 58. (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sanchez,L.M.: "Host selective suppression by water-soluble glucans from phytophthora spp.on hypersensitive cell death of suspension-cultured cells from some solanaceus plants caused by hyphal wall elicitors of the fungi" Ann.Phytopath.Soc,Japan. 58. 664-670 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 川北 一人: "植物の感染応答におけるシグナル伝達" 植物細胞工学. 14. 253-260 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 道家 紀志: "植物病原糸状菌レースと宿主品種間の寄生特異性の決定機構" 植物細胞工学. 15. 19-27 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sanchez,L.M.: "Involvement of suppressor-glucans and plant epidermal cells in host selective pathogenesis of Phytophthora capsici." J.Phytopathol.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sanchez,L.M.: "Elicitor-induced chemiluminescence in cell suspension cultures of tomato,sweet pepper and tobacco plants and its inhibition by suppressors from Phytophthora spp." Plant Science. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Doke,N.: "Involvement of superoxide in signal transduction:Response to attack by pathogens,physical and chemical shocks and U.V.irradiation.In:C.H.Foyer and P.M.Mullineaux(eds),Photooxidative Stress on Plants:Causes and Amelioration" CRC Press, (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Lee,S.S.: "Effect of hostーspesific toxins from Alternaria alternata strawberry pathotype on viability of strawberry cultured cells." Ann.Phytopath.Soc.Japan. 57. 671-679 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kawaguchi,I.: "Changes in amino acid and amine contents of susceptible tomato cultivars infected with Alternaria alternate tomato pathotype or treated with ALーtoxin" Ann.phytopath.Soc.Japan. 57. 526-533 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 道家 紀志: "植物ー病原糸状菌間相互作用の分子機構" 植物防疫. 45. 293-299 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Doke,N.: "E.J.Pell and K.L.Steffen(eds) Active Oxygen/Oxidative Stress and Plant Metabolism Involvemen of active oxygen in induction of plant defence response against infection and injury" American Soc.Plant Physiol., 286 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 道家 紀志: "久能 均編 生物相互間の認識機構Phytophthora属菌の水溶性グルカン:その病理的機能" 日本植物病理学会, 145 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 道家 紀志: "免疫処理による植物病害抵抗性の増強:ジャガイモ疫病" 植物防疫. 45. 101-105 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Xiao,J.Z: "Riceーspecific toxins produced by Bipolaris zeicola,race 3;evidnce for role as pathogenicity factors for rice and maize plants" Physiol.Mol.Plant Pathol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 奥 八郎(編集),道家紀志(分担執筆): "植物感染生理学最近の進歩 過敏感反応と情報伝達" 植物感染生理学最近の進歩刊行会, 287 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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