| Project/Area Number |
02454053
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Kobe University |
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Principal Investigator |
KATO Hajime Kobe Univ. Fac. of Agri. Professor, 農学部, 教授 (60221181)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥5,700,000 (Direct Cost: ¥5,700,000)
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| Keywords | Magnaporthe grisea / Mating type / Mating ability / Host range / millets |
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| Research Abstract |
Seeds and Pyricularia strains from Panicum miliaceum (Pm) and Setaria itarica (Si) collected from a mountainous area of Japan were used in order to examine the relationships of differentiation of pathogenicity.
l. Host range : Seven tribes of gramineous plants were utilized for inoculation tests. 1) Pm-strain attacked eighteen species among twenty seven. No pathogenicity was found against rice, Si and Eleusine coracana (Ec). No differentiated races were detected to Panicum miliaceum from Japan. 2) Si-strain attacked fourteen species among thirty one. There were races to Setaria itarica from Japan and China and no pathogenicity against rice, Pm and Ec.
2. Mating type : Standard isolates of mating types A and a of Ec were crossed with isolates of Pm and Si-strains to decide the mating type. 1) Pm-strain was classified into a-type (26 isoiates out of 39 isolates) and no A-type. 2) Si-strain was six A-type and nine a-type.
3. Mating ability : 1) Pm-strain was mated with Ec-strain, but ascospores were incomplete. Pm-strain was not mated with either rice-strain or Si-strain. 2) Si-strain was mated with both rice -strain and wheat-strain and some ascospores were fertile. Laggingchromosomes appeared 27 to 46% in the cross between Si-strain and Ec-strain and 24 to 35% in the cross between Si-strain and wheat-strain.
Based on these results, Pyricularia from Pm and Si has differentiated into two strains (forma specialis), though both plants have been raised in the same area.
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