Purification, identification and functional analysis of immune suppressive factol

• Project/Area Number: 02454065
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 製造化学・食品
• Research Institution: The University of Tokyo
• Principal Investigator: KAMINOGAWA Shuichi The Univ. of Tokyo Faculty of Agriculture, Professor, 農学部, 教授 (50011945)
• Project Period (FY): 1990 – 1991
• Project Status: Completed (Fiscal Year 1991)
• Budget Amount: ¥6,300,000 (Direct Cost: ¥6,300,000); Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 1990: ¥5,400,000 (Direct Cost: ¥5,400,000)
• Keywords: immune suppressive factor / IL-10 / suppressor T cell / immune tolerance / food allergen / 抑制因子

Research Abstract

CD8+ suppressor T cell clone 13G2 was established from lymph node of bovine si-casein immunized C57BL/6 mice. The clone suppressed proliferation of Thl clones induced by antigen plus antigen-presenting cells (APC) without any detectable cytolytic activity for responding cells. The suppression was not antigen specific and was not restricted to MHC. However, the clonec did not inhibit IL-2 induced Thl-proliferatiol) (1). The supernatant of 13G2 cultured with rIL-2 also suppressed antigen-induced proliferation of Thl clones, but did not that of Th2 clones. Target cells for the suppressor function was APC. Anti-IL-lO antibody SXC-I. blocked this suppressive activity. 13G2 clone produced IL-10 as revealed by Western blot analysis. This indicates that the suppressive lymphokine produced by 13G2 clone is IL-10 (2). A PCR analysis has shown that CD8+ cells of immunized lymph node express IL-10 mRNA upon antigenic stimulation. It is. possible that IL-10 play an important role in the immune suppression mediated by CD8+ lymphocytes.
(1) T. Hisatsune et al. (1990) J. lmmunol., 145 : 2421
(2) T. Hisatsune et al. (1992) Lymphokine & Cytokine Res., in press

Research Products

• [Publications] Hisatsune T.: "CD8+ suppressor T cell clone capable of inhibiting the antigen- and anti-T cell receptor-induced proliferation of Th clones without cytolytic activity" The Journal of Immunology. 145. 2421-2426 (1990)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hisatsune T.: "A suppressive lymphokine derived from Ts clone 13G2 is IL-10" Lymphokine and Cytokine Research. 11. 87-93 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hisatsuno T. et al.: "CD8+ suppressor T cell clone capable of inhibiting the antigen- and anti-T cell receptor-induced proliferation of Th clones without cytolytic activity" The Journal of Immunology. 145. 2421-2426 (1990)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hisatsune T. et al.: "A suppressive lymphokine derived from Ts clone 13G2 is IL-10." Lymphokine and Cytokine Research. 11. 87-93 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hisatsune T.: "CD8+ suppressor T cell clone capable of inhibiting the antigenーand antiーT cell receptorーinduced proliferation of Th clones without cytolytic activity" The Journal of Immunology. 145. 2421-2426 (1990)
• [Publications] Hisatsune T.: "A suppressive lymphokine derived from Ts clone 13G2 is ILー10" Lymphokine and Cytokine Research. 11. 87-93 (1992)
• [Publications] Hisatsune,T.et al: "CD8^+suppressor T cell clone capable of inhibiting the antigen and auti T cell receptorーinduced proliferation of Tn clones without cytolytic activity" J.gmmunol. 145. 2421-2426 (1990)