| Project/Area Number |
02454065
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAMINOGAWA Shuichi The Univ. of Tokyo Faculty of Agriculture, Professor, 農学部, 教授 (50011945)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | immune suppressive factor / IL-10 / suppressor T cell / immune tolerance / food allergen / 抑制因子 |
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| Research Abstract |
CD8+ suppressor T cell clone 13G2 was established from lymph node of bovine si-casein immunized C57BL/6 mice. The clone suppressed proliferation of Thl clones induced by antigen plus antigen-presenting cells (APC) without any detectable cytolytic activity for responding cells. The suppression was not antigen specific and was not restricted to MHC. However, the clonec did not inhibit IL-2 induced Thl-proliferatiol) (1). The supernatant of 13G2 cultured with rIL-2 also suppressed antigen-induced proliferation of Thl clones, but did not that of Th2 clones. Target cells for the suppressor function was APC. Anti-IL-lO antibody SXC-I. blocked this suppressive activity. 13G2 clone produced IL-10 as revealed by Western blot analysis. This indicates that the suppressive lymphokine produced by 13G2 clone is IL-10 (2). A PCR analysis has shown that CD8+ cells of immunized lymph node express IL-10 mRNA upon antigenic stimulation. It is. possible that IL-10 play an important role in the immune suppression mediated by CD8+ lymphocytes.
(1) T. Hisatsune et al. (1990) J. lmmunol., 145 : 2421
(2) T. Hisatsune et al. (1992) Lymphokine & Cytokine Res., in press
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