| Project/Area Number |
02454080
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SUZUKI Ryo Applied Biological Science, Professor, 生物生産学部, 教授 (70221330)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Heiske Applied Biological Science, Professor, 生物生産学部, 教授 (00034471)
ARAI Katsutoshi Applied Biological Science, Assistant Professor, 生物生産学部, 助教授 (00137902)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Pure line of fish / Aneuploidy / Gynogenetic diploid / Androgenetic diploid / Chromosome duplication / 純系実験魚 / 第二極体放出阻止 / 第1卵割阻止 / 紫外線照射精子 / 第2極体放出阻止 |
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| Research Abstract |
In order to obtain pure line of fish by producing gynogenetic and androgenetic diploids, optimum conditions for genetic inactivation of gametes and for chromosome duplication by suppression of the first cleavage were studied in the loach, Misgurnus anguillicaudatus. Chromosome spreads were analyzed to determine ploidy level at embryonic or early fry stages.
Gynogenetic diploids were obtained by pressure shocks applied 30-35 min after insemination with UV irradiated spermatozoa and lasting 60 sec, 800kg/cm^2, more than another conditions. Complete homozygosity of the fish was determined by genotypes on the locus with high rate of gene-centromere recombination. Androgenetic diploids were obtained from the eggs irradiated with UV and inseminated with normal spermatozoa, and them pressure-shocked with the same condition as in the case of gynogenesis. Survivals of gyno- and androgenetic fish was very low. Aneuploidy involving some fragments and haploidy were found in many gyno- and androgenetic embryos. This fact may be a factor responsible for low survival.
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