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Basic and Clinical Studies on abnormal expression of cytokine receptors in animals

Research Project

Project/Area Number 02454107
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionFaculty of Agriculture, University of Tokyo

Principal Investigator

HASEGAWA Atsuhiko  Fac. of Agri., Univ. Tokyo, Professor, 農学部, 教授 (90011923)

Co-Investigator(Kenkyū-buntansha) WATARI Toshihiro  Fac. of Agri., Univ. Tokyo, Assistant Professor, 農学部, 助手 (50220950)
GOITSUKA Ryo  Fac. of Agri., Univ. Tokyo, Assistant Professor, 農学部, 助手 (60205581)
ONO Kenichiro  Fac. of Agri., Univ. Tokyo, Associate Professor, 農学部, 助教授 (50111480)
SUZUKI Naoyoshi  Fac. of Agri., Univ. Tokyo, Professor, 農学部, 教授 (10003071)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsCytokine / Growth factor / Inflammation / Leukemia / Receptor / 腫瘍関連抗原 / B細胞 / レトロウイルス
Research Abstract

1. The leukemic cells obtained from a cat with reticuloendotheliosis, which is a very, primitive type of feline myeloproliferative diseases, constitutively expressed interleukin (IL)-2 receptor alpha-chain subunit.
2. The inoculation of feline infectious peritonitis (FIP) virus (FIPV) to cultured CRFK cells induced IL-6 production. Ascites from cats with FIP contained significant amounts of IL-1. Furthermore, IL-1 alpha and beta mRNA probes, the expression of franscripts were detected in peritoneal exudate cells and beta cDNA probes. By in situ hybridization with biotinylated human IL-1 alpha cDNA probes, the expression of IL-1 alpha mRNA was also confirmed in macrophage/monocytes infiltrated in the inflammatory lesions of FIP cats.
3. Administration of anti-murine IL-6 receptor antibody inhibited the IL-6-induced increase of platelet numbers and specific antibody responses.
4. The bovine B, Iymphoblastoid cell lines, BL2M3 and BL312 cells, released growth-promoting factors, and these cells also responded to the factors. The growth promoting factor-induced proliferation of BL2M3cells clearly i"nhibited by the addition of the antibody against one of the epitopes of tumor-associated antigen.
The production of cytokines and the expression of their receptors were analyzed in association with the pathogenesis of neoplasms and infections in domestic animals.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Goitsuka,R.: "Cytochemical and immunological properties of leukemic cells from a cat with reticuloendotheliosis." J. Vet. Med. Sci.53. 327-329 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Goitsuka,R.: "Characterization of a feline infectious peritonitis virus isolatc." J. Vet. Med. Sci.53. 337-339 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Goitsuka,R.: "Detection of interleukin 1 in ascites from cats with feline infectious peritonitis." J. Vet. Med. Sci.53. 487-489 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hasegawa,T.: "In situHybridization for the detection of feline interleukin alpha mRNA on the paraffin-embedded section using biotin-labeled probes." J. Vet. Med. Sci.53. 451-456 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hasegawa,T.: "Interleukin l alpha mRNA-expressing cells on the local inflammatory response in feline infectious peritonitis." J. Vet. Med. Sci.53. 995-999 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki,H.: "Anti-murine Il-6 receptor antibody inhibits IL-6 effects in vivo." Immunol.Letters. 30. 17-22 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ryo Goitsuka et. al.: "Cytochemical and immunological properties of leukemic cells from a cat with reticuloendotheliosis" J. Vet. Med. Sci.53. 327-329 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ryo Goitsuka et. al.: "Characterization of feline infectious peritonitis virus isolate" J. Vet. Med. Sci.53. 337-339 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ryo Goitsuka et. al.: "Detection of inteleukin 1 in ascites from cats with between feline infectious peritonitis" J. Vet. Med. Sci.53. 487-489 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi hasegawa et. al.: "In situ hybridization for the detection of feline interleukin 1 alpha mRNA on the paraffin-embedded section using biotin-labeled probes" J. Vet. Med. Sci.53. 451-456 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi hasegawa et. al.: "Interleukin 1 alpha mRNA-expressing cells on the local inflammatory response in feline infectious peritonitis" J. Vet. Med. Sci.53. 995-999 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroshi Suzuki et al.: "Anti-murine IL-6 receptor antibody inhibits IL-6 effects in vivo" Immunol. Letters. 30. 17-22 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mhan-pyo Yang et. al.: "Proliferative responses of a bovine leukemia virus-infected lymphoblastoid B-cell line by its culture supernatant and cytokines" J. Vet. Med. Sci.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mhan-pyo Yang et. al.: "Association of tumorassociated antigen on the proliferation of bovine leukemia virus-infected lymphoblastoid B-cell line" J. Vet. Med. Sci.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Goitsuka,R.: "Cytochemical and immunological properties of leukemic cells from a cat with reticuloendotheliosis." J.Vet.Med.Sci.53. 327-329 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Goitsuka,R.: "Characterization of a feline infectious peritonitis virus isolate." J.Vet.Med.Sci.53. 337-339 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Goitsuka,R.: "Detection of interleukin l in ascites from cats with feline infectious peritonitis." J.Vet.Med.Sci.53. 487-489 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hasegawa,T.: "In situHybridization for the detection of feline interleukin alpha mRNA on the paraffinーembedded Section using biotinーlabeled probes." J.Vet.Med.Sci.53. 451-456 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hasegawa,T.: "Interleukin l alpha mRNAーexpressing cells on the local inflammatory response in feline infectious peritonitis." J.Vet.Med.Sci.53. 995-999 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Suzuki,H.: "Antiーmurine ILー6 receptor antibody inhbits ILー6 effects in vivo." Immuol.Letters. 30. 17-22 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Goitsuka,R. et al.: "Establishment and characterization of a feline large granular lymphoma cell line expressing ILー2 receptor" Cancer Res.(submitted).

    • Related Report
      1990 Annual Research Report
  • [Publications] Yang,M. et al.: "A bovine lymphoblastoid B cell line,BL2M3,releases and responds to its growth factor" Vet.Immunol.Immunopathol.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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