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Immunohistochemical and ultrastructural study of GRP-containing nerves in the gastric mucosa

Research Project

Project/Area Number 02454111
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNiigata University

Principal Investigator

FUJITA Tsuneo  Niigata University, School of Medicine, Professor, 医学部, 教授 (00032863)

Co-Investigator(Kenkyū-buntansha) KANAZAWA Hiroaki  Niigata University, School of Medicine, Assistant, 医学部, 助手 (40214431)
IWANAGA Hiromi  Niigata University, School of Medicine, Assistant, 医学部, 助手 (30193759)
IWANAGA Toshihiko  Niigata University, School of Medicine, Associate Prof., 医学部, 助教授 (10160128)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsGRP / Gastrin-releasing peptide / Immunohistochemistry / Gastric mucosa / Peptic ulcer / Microcirculation / Autonomic neurons / Brain-gut hormones / シナプス小胞 / GRP(ガストリン放出ペプチド) / 潰瘍 / セロトニン合成酵素 / 肥満細胞
Research Abstract

1. Innervation of the oxyntic mucosa : The gastric mucosa of mammalian species is heavily innervated by several types of peptidergic nerve fibers : gastrin-releasing peptide (GRP)-containing nerves being most predominant among them. Cholinesterase-positive nerves were densely distributed in the rat gastric mucosa and identical to the GRP nerves, while adrenergic nerves were few.
2. Ultrastructure of GRP-containing nerves : The immunoreactivity for GRP was selectively localized in large cored vesicles gathering in the swollen parts of nerve fibers. Numerous small clear vesicles free of the immunoreactivity were interm : Engled with the large cored vesicles, suggesting the colocalization of GRP and other transmitter with a small molecular weight, possibly acetylcholine.
3. Stress-induced morphological change of GRP nerves : The decreased GRP immunoreactivity in the nerves was ultrastructurally confirmed by the present observation showing marked decrease in number of the GRP-containing large cored vesicles. Simultaneously, small clear vesicles decreased in number, suggesting the corelease of GRP and acetylcholine under stress condition.
4. Topographic relation of GRP nerves and blood vessels : The GRP nerves were shown under SEM to be closely related with capillaries, but not with any glandular cells. The capillaries were accompanied by pericytes which are contractile in nature, thus being able to contract, finally causing mucosal ischemia and damage. Arterioles around the lamina muscularis mucosas might be also important in regulation of blood flow, since they suppiyed the mucosal capillaries in the lamina propria and were densely innervated by vasomotor neurons.
5. Mast cells in the gastric mucosa : Mast cells in rodents contain serotonin as well as histamine. However, they in the gastric mucosa did not possess a synthesizing enzyme for serotonin, in contrast to peritoneal mast cells.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 岩永 敏彦: "種々の実験潰瘍における胃体部GRP神経の動態について" 消化管ホルモン. 9. 330-334 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩永 敏彦: "胃体部粘膜における神経網の分布と微細構造" 消化管ホルモン. 10. 472-476 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga Toshihiko: "GRP (gastrin-releasing peptide)-containing nerves in the rat stomach and stress-induced depletion of their synaptic vesicles.An electron microscopic study." Arch Histol.Cytol.54. 573-580 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩永 敏彦: "潰瘍発生のメカニズムー神経と血管の分布から考える" 消化性潰瘍ー臨床と基礎. 10. 140-151 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohtsuka Hiroyuki: "Immunohistochemical localization of tryptophan hydroxylase and serotonin in the gastrointestinal tract of mice." Biomed.Res.12. 131-142 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩永 ひろみ: "ラット胃粘膜血管の微細構造と血流調節" 新潟県医師会報. 500. 1-6 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩永 敏彦: "胃体部粘膜の神経におけるGRPとアセチルコリンの共存および同時放出に関する形態学的研究" 消化管ホルモン. 11. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga, Toshihiko: "GRP-immunoreactive neurons in the rat oxyntic mucosa with special reference to experimentally induced peptic ulcers." Gut Hormones. 9. 330-334 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga, Toshihiko: "Distribution and ultrastructure of nerve fibers in the mucosa of the gastric body." Gut Hormones. 10. 472-476 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga, Toshihiko: "GRP (gastrin-releasing peptide) -containing nerves in the rat stomach and stress-induced depletion of their synaptic vesicles. An electron microscopic study." Arch. Histol. Cytol.54. 573-580 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga, Toshihiko: "Mechanism of ulcer formation with special reference to innervation and blood supply." Peptic Ulcer. 10. 140-151 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohtsuka, Hiroyuki: "Immunohistochemical localization of tryptophan hydroxylase and serotonin in the gastrointestinal tract of mice." Biomed. Res.12. 131-142 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga, Hiromi: "Ultrastructure of blood vessels and their regulation in the stomach of rats." J. Niigata Med. Assoc.500. 1-6 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga, Toshihiko: "A ultrastructure study on the colocalization and co-release of GRP and acetylcholine in the nerve fibers of the oxyntic mucosa of the rat." Gut Hormones. 11. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwanaga T.: "GRP(gastrinーreleasing peptide)ーcontaining nerves in the rat stomach and streeーinduced depletion of their synaptic vesicles." Arch.Histol.Cytol.54. 573-580 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩永 敏彦: "潰瘍発生のメカニズムー神経と血管の分布から考える" 消化性潰瘍ー臨床と基礎. 10. 140-151 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩永 敏彦: "胃体部粘膜の神経におけるGRPとアセチルコリンの共在および同時放出に関する形態学的研究" 消化管ホルモン. 11. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩永 ひろみ: "ラット胃粘膜血管の微細構造と血流調節" 新潟県医師会報. 500. 1-6 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩永 敏彦,藤田 恒夫: "種々の実験潰瘍における胃体部GRP神経の動態について" 消化管ホルモン. 9. 330-334 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohtuka H.,Iwanaga T.,Fujita T.,et al.: "Immunohistochemical demonstration of tryptophan hydroxylase and serotonin in the gastrointestinal tract of mice." Biomedical Research. 12. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岩永 敏彦,藤田 恒夫: "胃体部粘膜の神経網について" 消化管ホルモン. 10. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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