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Glutamate receptor channels in hippocampal neurons and their development

Research Project

Project/Area Number 02454119
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionGunma University

Principal Investigator

OZAWA Seiji  Gunma University, School of Medicine, Professor, 医学部, 教授 (40049044)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1990: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsGlutamate receptor / Hippocampal neuron / AMPA / Kainate / NMDA / Calcium ion / Rectification / Excitatory synapse / 整流特性 / GluR2 / 海馬 / カルシウムイオン透過性 / 培養ニュ-ロン / キスカル酸受容体 / カイニン酸受容体
Research Abstract

Glutamate receptor channels in the mammalian central nervous system are classified into three subtypes; NMDA, AMPA/kainate (KA) and KA-selective receptor channels. AMPA/KA receptor channels are activated by both AMPA and KA. In this study, we investigated mainly the properties of AMPA/KA receptors and their development in cultured rat hippocampal neurons prepared from 17- to 19-day-old rat embryos. The results are summarized as follows. 1) Cultured hippocampal neurons became sensitive to KA and AMPA within 7 days after plating. This is due to the development of AMPA/KA receptors, but not of KA-selective receptors. Thereafter, the amplitude of KA- or AMPA-induced current increased gradually. 2) AMPA/KA receptors were classified into two types. The type I receptor exhibited an outwardly-rectifying current-voltage (I-V) relation with little Ca^<2+> permeability. In contrast, the type II receptor was characterized by a remarkable inward rectification and a prominent Ca^<2+> permeability. C … More ombining whole-cell patch-clamp recordings and the PCR amplification of the mRNA harvested from a single neuron, we determined the subunit composition of the type I and type II AMPA/KA receptors expressed in hippocampal neurons. The type I receptor was composed of the GluR1 and GluR2 subunits, whereas the type II receptor of the GluR1 and GluR4 subunits. 3) The type II AMPA/KA receptor appeared exclusively in small neurons with elliptical somata and fine neurites throughout 1-5 weeks after plating. These neurons seem to be GABA ergic interneurons, since GAD was detected in these neurons. 4) When the excitatory synapse was established between dissociated CA1 pyramidal cells and neurons from the CA3/CA4 regions, high-density AMPA spots were detected on the apical dendrite of the postsynaptic CA1 neuron. Both the fast EPSC and the AMPA response on these spots showed almost linear I-V relations. This suggests that the type I AMPA/KA receptor is more responsible for generating the fast EPSC in this excitatory synapse than the type II AMPA/KA receptor. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ozawa,S.et al.: "Two types of kainate response in cultured rat hippocampal reurons." Journal of Neurophysiology. 66. 2-11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozawa,S.et al.: "Excitatory synapse in the rat hippocampus in tissue culture and effects of aniracetam." Neuroscience Research. 12. 72-82 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小澤 瀞司: "中枢ニューロンのグルタミン酸受容体チャネル" 膜. 16. 258-269 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 小澤 瀞司: "グルタミン酸レセプターのCa^<2+>透過性" 実験医学. 10. 611-618 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsuzuki,K,et al.: "Agonist-and subunit-dependent potentiation of glutamate receptors by a nootropic drug anirracetam." Molecular brain Research. 16. 105-110 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozawa,S.: "Glutamate receptor channels in hippocampal neurons." Japanese Journal of Physionlogy.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozaea,S.et al.: "Two distinct types of responses to kainate and AMPA in cultured hipoocampal neurons." In:Excitatory Amino Acids.Vol.9,edites by R.P.Simom,THieme,New York, 7 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozawa,S. et al.: "Two types of kainate response in cultured rat hippocampal neurons." Journal of Neurophysiology. 66. 2-11 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozawa,S. et al.: "Excitatory synapse in the rat hippocampus in tissue culture and effects of aniracetam." Neuroscience Research. 12. 72-82 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozawa,S. et al.: "Two distinct types of responses to kainate and AMPA in cultured hippocampal neurons." Excitatory Amino Acids. 9. 117-123 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsuzuki,K. et al.: "Agonist- and subunit-dependent potentiation of glutamate receptors by a nootropic drug aniracetam." Molecular Brain Research. 16. 105-110 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ozawa,S.: "Glutamate receptor channels in hippocampal neurons." Japanese Journal of Physiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsuzuki,K.et al: "Agonist- and subunit-dependent potentiation of glutamate receptors by a nootropic drug aniracetam." Molecular Brain Research. 16. 105-110 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ozawa,S.: "Glutamate receptor channels in hippocampal neurons." Japanese Journal of Physiology in press.

    • Related Report
      1992 Annual Research Report
  • [Publications] 小澤 瀞司: "培養海馬ニューロンのグルタミン酸受容体チャネル" 神経精神薬理. 15. 109-117 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Seiji Ozawa et al.: "Two types of kainate response in cultured rat hippocampal neurons." Journal of Neurophysiology. 66. 2-11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seiji Ozawa et al.: "Excitator^^・y synapse in the rat hippocampus in tissue culture and effects of aniracetem." Neuroscience Research. 12. 72-82 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seiji Ozawa et al.(ed.by R.P.Simon.): "Two distinct types of responses to kainate and AMPA in cultured hippocampal neurons.In Excitatory Amino Acids(Vol.9)." Thieme Medical Publishers,Inc.New York, 7 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seiji Ozawa: "Permeation of calcium through glutamate receptor channels:Two types of kainate/AMPA response." Biomedical Research.

    • Related Report
      1990 Annual Research Report
  • [Publications] Seiji Ozawa et al.: "Two types of kainate response in cultured rat hippocampal neurons." Journal of Neurophysiology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Seiji Ozawa et al.: "Permeation of calcium ions through receptor channels activated by NMDA and related agents.<In>___ー NMDA Receptor Related Agents:Biochemistry,Pharmacology and Behavior.ed.by K.Kameyama et al." NPP Books,Ann Arbor,MI,USA.,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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