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The Role of Protein Phosphorylation and dephosphorylation in Neuronal Signal Transduction

Research Project

Project/Area Number 02454137
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKobe University

Principal Investigator

TANAKA Chikako  Kobe Univ. Sch. Med., Professor, 医学部, 教授 (20025571)

Co-Investigator(Kenkyū-buntansha) SHUNTOH Hisato  Kobe Univ. Sch. Med., Research Associate, 医学部, 助手 (70206259)
SAITO Naoaki  Kobe Univ. Sch. Med., Associate Professor, 医学部, 講師 (60178499)
KUNO Takayoshi  Kobe Univ. Sch. Med., Associate Professor, 医学部, 助教授 (50144564)
谷山 紘太郎  長崎大学, 医学部, 教授 (70030898)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥6,300,000 (Direct Cost: ¥6,300,000)
KeywordsProtein Phosphorylation / Dephosphorylation / Protein Kinase / Calcineurin / Synapse Formation / Neurotransmitter / cDNA Cloning / 蛋白質燐酸化酵素 / 蛋白質脱燐酸化酵素 / 神経細胞 / サブタイプ / 免疫組織化学 / プロテインキナ-ゼC
Research Abstract

Recently, protein phosphorylation and dephosphorylation are thought to be a central event in cellular signal transduction. Large amount of protein kinases and protein phosphatases are found in the central nervous system, suggesting that these enzymes are involved in the neuronal signal transduction. In this fiscal year, we obtained following findings on the protein kinase C and calcineurin.
1) Developmental change in the distribution of protein kinase C subspecies in rat brain was examined. alpha and beta I subspecies were found when rat was born, on the other hand, beta II and gamma subspecies appeared one to two weeks after the birth when the synaptogenesis occurred.
2) Immunohistochemical study on the localization of four protein kinase C subspecies and neurotransmitters in striate-nigral system was performed. Dopaminergic neurons in the substantia nigra and cholinergic neurons in the striatum contained alpha subspecies, and GABAergic neurons in the striatum contained beta -I subspecies.
3) Distribution of calcineurin A subtypes were examined using in situ hybridization technique and immunohistochemistry in rat brain. The activation mechanism of calcineurin was studied usign calmodulin antagonists.
4) GABA_B receptor was expressed in Xenopus oocyte and its regulation by protein kinase C was demonstrated.
5) cDNA cloning of testis-specific calcineurin B-like protein and cDNA cloning of yeast homolog of calcineurin B were performed.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] M.Hirata: "Differential expression of the βI- and βII-PKC subspecies in the postnatal developing rat brain;an immunocytochemical study." Dev.Brain Research. 62. 229-238 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] C.Yoshihara: "Differential localization of four subspecies of protein kinase C in the rat striatum and substantia nigra." J.neurosci.11. 690-700 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Mukai: "Calmodulin antagonists differentiate between Ni^<2+>-and Mn^<2+>- stimulated phosphatase activity of calcineurin." J.Biochem.110. 402-406 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Takaishi: "Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain." Biochem.Biophys.Res.Commun.174. 393-398 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Taniyama: "Expression of the GABA^B receptor in Xenopus oocytes and inhibition of the response by activation of protein kinase C." FEBS Lett.278. 222-224 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Kuno: "cDNA cloning of a calcineurin B homolog in saccharomyces cerevosoae." Biochem.Biophys.Res.Commun.180. 1159-1163 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Taniyama: "Advances in Experimental Medicine and Biology" Plenum Press, 8 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田中 千賀子: "〈代謝〉シグナル伝達路とそのクロスト-ク" 中山書店, 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Hirata: "Differential expression of the beta I-and beta II-PCK subspecies in the postnatal developing rat brain" Dev. Brain Research. 62. 229-238 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] C. Yoshihara: "Differential localization of four subspecies of protein kinase C in the rat striatum and substantia nigra. an immunocytochemical study." J. Neurosci.11. 690-700 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Mukai: "Calmodulin antagonists differentiate between Ni^<2+>-and Mn^<2+>-stimulated phosphatase activity of calcineurin." J. Biochem.110. 402-406 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takaishi: "Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain." Biochem. Biophys. Res. Commun.174. 393-398 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Taniyama: "Expression of the GABA_B receptor in Xenopus oocytes and inhibition of the response by activation of protein kinase C." FEBS Lett.278. 222-224 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Kuno: "cDNA cloning of a calcineurin B homolog in saccharomyces cerevisiae." Biochem. Biophys. Res. Commun.180. 1159-1163 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Taniyama: "Expression of the GABA_B receptor in Xenopus oocytes and desensitization by activation of protein kinase C." Adv. Experimental Med. Biol.287. 413-420 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] C. Tanaka: "The review on signal transduction and crosstalks. (Signal Transduction and Crosstalks)" Taisha. 374. 1-10 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Hirata: "Differential expression of the βIー and βIIーPKC subspecies in the postnatal developing rat brain;an immunocytochemical study." Dev.Brain Research. 62. 229-238 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] C.Yoshihara: "Differential localization of four subspecies of protein kinase C in the rat striatum and substantia nigra." J.Neurosci.11. 690-700 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Mukai: "Calmodulin antagonists differentiate between Ni^<2+>ー and Mn^<2+>ー stimulated phosphatase activity of calcineurin." J.Biochem.110. 402-406 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Takaishi: "Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain." Biochem.Biophys.Res.Commun.174. 393-398 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Taniyama: "Expression of the GABA_B receptor in Xenopus oocytes and inhibition of the response by activation of protein kinase C." FEBS Lett.278. 222-224 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Kuno: "cDNA clonig of a calcineurin B homolog in saccharomyces cerevisiae." Biochem.Biophys.Res.Commun.180. 1159-1163 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Taniyama: "Advances in Experimetal Medicine and Biology" Plenum Press, 8 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 田中 千賀子: "〈代謝〉シグナル伝達路とそのクロスト-ク" 中山書店, 11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Ito: "Immunocytochemical localization of the α subspecies of protein kinase C in rat brain" Proc.Natl.Acad.Sci.USA. 87. 3195-3199 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Kose: "Electron microscopic localization of γーand β IIーsubspecies of protein kinase C in rat hippocampus" Brain Res.518. 209-217 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Mori: "Immunocytochemical localization of protein kinase C subspecies in the rat spinal cord:Light and electron microscopic study" J.Comp.Neurol.299. 167-177 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] C.Yoshihara: "Differential localization of four subspecies of protein kinase C in the rat striatum and substantia nigra." J.Neurosci.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Takaishi: "Differential distribution of the mRNA encoding two isoforms of the catalytic subunit of calcineurin in the rat brain" Biochem.Biophys.Res.Commun.174. 393-398 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Taniyama: "Expression of the GABAB receptor in Xenopus oocytes and inhibition of the response by activation of protein kinase C" FEBS. 278. 222-224 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nishizuka: "Advances in second messenger and phosphoprotein researchーThe biology and medicine of signal transduction" Raven press, 750 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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