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Molecular, cellular and pathological analysis of structure and function of macrophages

Research Project

Project/Area Number 02454169
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionOita Medical University

Principal Investigator

YAMAMOTO Shunsuke  Oita Medical Univ.,Pathology,Professor, 医学部, 教授 (90040188)

Co-Investigator(Kenkyū-buntansha) SETOGUCHI Mihoko  Oita Medical Univ.,Pathology,Assistant, 医学部, 助手 (20236110)
YOSHIDA Seiji  Oita Medical Univ.,Pathology,Assistant, 医学部, 助手 (70182764)
AKIZUKI Shinishiro  Oita Medical Univ.,Pathology,Assistant, 医学部, 助手 (80159334)
樋口 安典  大分医科大学, 医学部, 助教授 (60040284)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsCD14 / MS2 / osteopontin / cDNA / genome / expression / recombinant / mouse and human / オステオポンチン / マクロファ-ジ遺伝子 / マクロファ-ジcDNA / マクロファ-ジ / 膜蛋白 / 分泌蛋白 / Sppー1
Research Abstract

1.MS2:(1). Complete nucleotide sequences of the MS2 gene and its 5 upstream region were determined. (2). cis-regulatory element for MS2 expression in macrophages (Mphi) was investigated. (3). Significant homology of MS2 with hemorrhagic snake venom proteins were found. (4). Recombinant proteins for MS2 proteins (rMS2) and poly clonal and monoclonal antibodies for rMS2 (anti- rMS2) were produced. (5). PAP and FACS analysis revealed that MS2 was expressed in Mphi and neutrophils but not in lymphocytes and mast cells. (6). cDNA for the human homologue of MS2 was separated.
2. CD14:(1). The 5'upstream nucleotide sequences of the mouse and human CD14 genes were determined. (2). Promoter and enhancer regions for the mouse CD14 gene were defined. (3). Mouse rCD14 and polyclonal and monoclonal antibodies against rCD14 (anti- rCD14) were produced. PAP analysis using anti-rCD14 demonstrated CD14 in myelomonocytic cells but not in lymphocytes and mast cells. (5). TNFalpha production and LPS-induced shock were significantly suppressed with anti-rCD14.
3. OP:(1). The 5'upstream nucleotide sequences of the mouse and human OP genes were determined. (2). Complete nucleotide sequence of the human OP gene was determined. (3). Cis regulatory sequences of the mouse and human OP genes were determined. (4). rOP was produced using E. coli and vaculovirus vector systems. (5). rOP significantly suppressed haptotaxis of Mphi and melanoma (B16-BL) cells, and lung metastasis of B16-BL cells. (5). Antibodyagainst rOP was produced.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Nobuyoshi Nasu: "Molecular and physiological properties of murine CD14" Int Immunol.3. 205-213 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 山本 俊輔: "顆粒球・単球系細胞の高度LPS刺激系-CD14とLPS結合蛋白の遺伝子および蛋白の構造と機能" 免疫91代謝. 28. 131-140 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 山本 俊輔: "分泌リン蛋白-1(オステオポンチン)-構造と機能および利用について" Bio Industory. 8. 319-327 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Keiko Matsuura: "Identification of a tissue-specific regulatory element within the murine CD14 gene" J.Biol.Chem.267. 21718-21794 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 山本 俊輔: "マクロファージ特異分子" 臨床免疫. 24. 1047-1058 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nasu, N., Yoshida, S., Akizuki, S., Higuchi, Y., Setoguchi, M. and Yamamoto,S: "Molecular and physiological properties of murine CD14." Int. Immunol. 3(2). 205-213 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamamoto, S., Setoguchi, M., Matsuura, K. and Higuchi, Y.: "Highly sensitive system for LPS-stimulation in myelomonocytic cells-Structure and function of genes and proteins for CD14 and LPS-binding protein." Metabolism and Disease. 28(12). 131-140 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamamoto, S. and Setoguchi, M.: "Secreted phosphoprotein-1(osteopontin)-Structure and function, and its use." Bio Industry. 8(5). 319-327 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuura, K., Ishida, T., Setoguchi, M., Higuchi, U., Akizuki, S., and Yamamoto, S.: "Identification of a tissue-specific regulatory element within the murine CD14 gene" J. Biol. Chem.267(30). 21718-21794 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamamoto, S., Higuchi, Y., Akizuki, S., Yoshiyama, K.: "Macrophage-specific genes." Rinsyome neki. 24(7). 1047-1058 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nobuyoshi Nasu: "Molecular and physiological properties of murine CD14" Int Immunol.3. 205-213 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山本 俊輔: "顆粒球・単球系細胞の高度LPS刺激系ーCD14とLPS結合蛋白の遺伝子および蛋白の構造と機能" 免症91 代謝. 28. 131-140 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山本 俊輔: "分泌リン蛋白ー1(オステオポンチン)ー構造と機能および利用について" Bio Industory. 8. 319-327 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Keiko Matsuura: "Identification of a tissue-specific regulatory element within the murine CD14 gene" J.Biol.Chem. 267. 21718-21794 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山本 俊輔: "マクロファージ特異分子" 臨床免疫. 24. 1047-1058 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nasu,N.: "Molecular and physiological pro娜erties of murine CD14." Int.Immunol.3. 205-213 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 山本 俊輔: "分泌リン蛋白ー1(オステオポンチン)構造と機能および利用について" バイオインダストリ-. 5. 319-327 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 山本 俊輔: "顆粒球・単球系細胞の高感度LPS刺激系CD14とLPS結合蛋白質の遺伝子および蛋白質の構造と機能" 代謝. 131-140 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuura,K.: "Identification of a tissueーspecific regulatory element within the murine CD14 gene" J.Biol.Chem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshiyama,K.: "Structure of the MS2 gene strongly expressed in murine monocytic lineage A unique transmembrane protein containing cysteinーrich domain" J.Biol.Chem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Higuchi,Y.: "Inhibition of fibronectinーinduced haptotactic migration of murine melanoma B16 by osteopnotin"

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuura,K.: "Nucleotide and amino acid sequences of the mouse CD14 gene." Nucleic Acids Res.17. 2132 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Setoguchi,M.: "Mouse and human CD14(myeloid cellーspecific leucineーrich glycoprotein)Primary structure deduced from cDNA clones." Biochim.Biophys.Acta.1008. 213-222 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyazaki,Y.: "Nucleotide sequence of cDNA for mouse osteopontinーlike protein" Nucleic Acids Res.17. 3298-3298 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshida,S.: "Molecular cloning of cDNA encoding MS2 antigen,a novel cell surface antigen strongly expressed in murine monocytic lineage." Int.Immunol.2. 585-591 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyazaki,Y.: "The mouse osteopontin gene Expression in monocytic lineages and complete nucleotide sequence" J.Biol.Chem.265. 14432-14438 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsuura,K.: "Letters to the editor." J.Immunol.145. 3150-3150 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nasu,N.: "Molecular and physiological properties of murine CD14." Int.Immunol.3. 205-213 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 山本 俊輔: "分泌リン蛋白ー1(オステオポンチン)構造と機能および利用について" バイオインダストリ-. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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