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Analysis of a common mechanism for the induction of cell-mediated immunity by intracellular bacteria and its application to host defense against bacterial infection.

Research Project

Project/Area Number 02454176
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionNiigata University

Principal Investigator

MITSUYAMA Masao  Niigata University, School of Medicine, Professor, 医学部, 教授 (10117260)

Co-Investigator(Kenkyū-buntansha) KAWAMURA Ikuo  Niigata University School of medicine, Assistant Researcher, 医学部, 助手 (20214695)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsIntracellular bacteria / Listeria monocytogenes / BCG / Protective immunity / Cytokine / Macrophage / 感染抵抗性 / 抗酸菌 / PCR / T細胞 / CD4 / 遅延型過敏反応 / マクロファ-ジ / インタ-フェロンーγ / インタ-ロイキンー1
Research Abstract

This study was carried out to analyze the common mechanisms involved in the induction of cell-mediated immunity to intracellular bacteria including Listeria and Mycobacteria, and the following results were obtained. (1)The ineffectiveness of killed bacterial cells in the induction of specific immunity is not attributable to the amount of antigen or to the peristence of bacterial antigen in mice. (2)T cells generated in mice after immunization with viable bacteria could be activated by both viable and killed bacterial cells and the presence of HSP is not responsible for the induction of protective T cells. (3)Among various strains of L.monocy- togenes, some strains were found to be ineffective for the induction of specific immunity even if used as viable bacterial vaccine. The ability to induce protective immunity was correlated with the ability of each strain to induce macrophage production of IL-1. (4)Administration of recombinant IL-1 along with immunization with killed bacteria significantly promoted the development of antigen-specific T cells in mice. (5)The IL-1-inducing ability of Listeria was attributable to the production of 58KDa-hemolysin, listeriolysin O. (6)Production of IFN-gamma was indispensable for the expression of protective immunity by antigen- specific CD4 T cells and those which produce cytokine other than IFN-gamma could not afford the protective immunity.
These results indicated that monokine production including IL-1 is critically required in the induction of cell-mediated immunity to intracellular bacteria and that IFN-gamma-producing ability is indispensable for the final expression of protective immunity by antigen-specific CD4^+ T cells.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Mitsuyama,Masao: "Difference in the induction of macrophage interleukin-1 production between viable and killed cells of Listeria monocytogenes." Infection and Immunity. 58. 1254-1260 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Igarashi,Ken'ichi: "Interleukin-1-induced promotion of T cell differentiati-on in mice immuniozed with killed Listeria monocytogenes" Infection and Immunity. 58. 3973-3979 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsukada,Hiroki: "Dissociated development of T cells mediating delayed-type hypersensitivity and protective T cells against Listeria monocytogenes and their functional difference in lymphokine production." Infection and Immunity. 59. 3589-3595 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsukada,Hiroki: "Induction of macrophage interleukiy-1 production by Listeria monocytogenes hemolysin." Cellular Immunology. 140. 21-30 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawamura,Ikuo: "IFN-γ-producing ability as a possible marker for the protective T cells against Mycobacterium bovis BCG" Journal of Immunology. 148. 2887-2893 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshikawa,Hiroko: "Membrane damage and interleukin-1 production in murine macrophages exposed to listeriolysin O." Infection and Immunity. 61. 4 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Mitsuyama, Masao et al.: "Difference in the induction of macrophage interleukin-1 production between viable and killed cells of Listeria monocytogenes." Infection and Immunity. 58(5). 1254-1260 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Igarashi, Kenichi et al.: "Interleukin-1-induced promotion of T cells differentiation in mice immunized with killed Listeria monocytogenes." Infection and Immunity. 58(12). 3973-3979 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsukada, Hiroki et al.: "Dissociated development of T cells mediating delayed-type hyper-sensitivity and protective T cells against Listeria monocytogenes and their functional difference in lymphokine production." Infection and Immunity. 59(10). 3589-3595 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tsukada,Hiroki et al.: "Indcution of macrophage interleukin-1 production by Listeia monocytogenes homolysin." Cellular Immunology. 140(1). 21-30 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kawamur, Ikuo et al.: "IFN-gamma-prodcuing ability as a possible marker for the protective T cells against Mycobacterium bovis BCG in mice." Journal of Immunology. 148(9). 2887-2893 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshikawa,Hiroko et al.: "Membrane damage and interleukin-1 production in murine macrophages exposed to listeriolysin O." Infection and Immunity. 61(4). (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Serushago,Basir: "Difference in the functional maturation of T cells against Listeria monocytogenes in lymph nodes and spleen." Immunology. 75. 238-244 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tsukada,Hiroki: "Induction of macrophage interleukin-1 production by Listeria monocytogenes hemolysin." Cellular immunology. 140. 21-30 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Serushago,Basir: "Effect of recombinant human granuloscyte-colony stimulating factor on murine resistance against Listeria monocytogenes." Immunology. 75. 475-480 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kawamura,Ikuo: "IFN-γ-producing ability as a possible marker for the protecti-ve T cells against Mycobacterium bovis BCG in mice." Journal of Immunology. 148. 2887-2893 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujio,hiroshi: "Investigation of the role of macrophage and endogenous interferon-g in natural resistance of mice against Legionella penumophila." FEMS Microbiology and Immunology. 89. 183-192 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yoshikawa,Hiroko: "Membrane damage and interleukin-1 production in murine macropahges exposed to listerioylsin O." Infection and Immunity. 61. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Katsumi Muramori: "A dissociated induction of MCFーproducing and MAFーproducing T cells specific for Listeria monocytogenes in the in vitro primary culture system" Immunology. 72. 373-379 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kokichi Goto: "Mycolic acidーcontaining glycolipid as a possible virulence factor of Rhodococcus equi for mice." Microbiology and Immunology. 35. 175-185 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshiya Kobayashi: "The analysis of the defense mechanisms against indigeneous bacterial translocation in Xーirradiated mice." Microbiology and Immunology. 35. 315-334 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroki Tsukada: "Dissociated development of T cells mediating delayedーtype hypersensitivity and protective T cells and their functional difference in lymphokine production" Infection and immunity. 59. 3589-3595 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroki Tsukada: "Induction of macrophage interleukinー1 production by Listeria monocytogenes hemolysin" Cellular Immunology. 140. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ikuo Kawamura: "Gamma interferonーproducing ability as a possible marker for the protective T cells against Mycobacterium bovis BCG in mice" Journal of Immunology. 148. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ikuo Kawamura,Masao Mitsuyama & Kikuo Nomoto: "Enhanced protection of cyclophosphamideーtreated mice against infection with Pseudomonas aeruginosa after treatment with Zー100,a polysaccharideーrich extract of Mycobacterium tuberculosis Aoyama B." Immunopharmacology and Immunotoxicology. 12. 331-343 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masao Mitsuyama,Kenーichi Igarashi,Ikuo Kawamura,et al.: "Difference in the induction of macrophage interleukinー1 production between viable and killed cells of Listeria monocytogenes." Infection and Immunity. 58. 1254-1260 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kenーichi Igarashi,Masao Mitsuyama,Katsumi Muramori,Hiroki Tsukada,Kikuo Nomoto: "Interleukinー1ーinduced promotion of Tーcell differentiation in mice immunized with killed Listeria monocytogenes." Infection and Immunity. 58. 3973-3979 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Katsumi Muramori,Masao Mitsuyama,Toshiya Handa,Bazir Serushago,et al.: "A dissociated induction of MCFーproducing and MAFーproducing T cells specific for Listeria monocytogenes in the in vitro primary culture system." Immunology. 72. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kokichi Goto,Masao Mitsuyama,Sadao Imaizumi,Ikuo Kawamura & Ikuya Yano: "Mycolic acidーcontaining glycolipid as a possible virulence factor of Rhodococcus equi for mice." Microbiology and Immunology. 35. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroki Tsukada,Ikuo Kawamura,Takao Fujimura,Kenーichi Igarashi & M.Mitsuyama: "Induction of macrophage interleukinー1 production by Listeria monocytogenes hemolysin." Infection and Immunity. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 光山 正雄(分担): "シンプル微生物学" 南江堂, 314 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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