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Hypothesis: Both appearance and disappearance of viruses are controlled by the accumulation of amino acid substitution in receptor binding domain

Research Project

Project/Area Number 02454184
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNational Institute of Health

Principal Investigator

TAKEDA Naokazu  National Institute of Health, Department of Epidemiology, Chief, 感染症疫学部, 室長 (90132894)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Shudo  National Institute of Health, Deputy Director General, 副所長 (20072902)
MIYAMURA Kikuko  National Institute of Health, Department of Epidemiology, Chief, 感染症疫学部, 室長 (40072897)
TANIMURA Masako  National Children's Medical Research Center, 小児生態部, 研究員 (90014191)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsPicornaviruses / Enterovirus 70 / Molecular evolution / Phylogenetic tree / Nucleotide substitution / Receptor / コクサッキ-ウイルスA24変異株 / 塩基配列
Research Abstract

The genome of the RNA viruses is known to evolve 10^6 times faster than ordinary DNA genome. This higher evolutionary rate is thought to be linked with both appearance and disappearance of the viruses in human world. To understand the interaction between cellular receptor molecule and receptor binding domain of the virion the nucleotide and amino acid substitutions in virus encoded proteins were analyzed. According to the nucleotide sequences of EV70 capsid protein VP1 we have constructed a fine phylogenetic tree. By analyzing the phylogenetic relationship and the substitution pattern among the isolates we deduced the original nucleotide sequence. The evolutionary rate was estimated to be 5x10^<-3>/site/year, whereas the rate of the 3C protease region of coxsackievirus A24 variant, an another agent that cause acute hemorrhagic conjunctivitis, was calculated to be 4.9x10^<-3>/site/year. Using a monoclonalantibody that is capable of blocking EV70 infection screening of the COS cells transfected with cDNA library derived from Hela cell mRNA was carried out by socalled "panning" method. The receptor molecules are found to be located on the surface of the susceptive cells and has the nucleotide sequence identical to that of the molecule which regulates a function of an immunologically important molecule.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] H.Ishiko et al: "Phylogenefic Analysis of a Coxsackievirus A24 Vaviant: The Most Recent Worldwide Pandemic Was Caused by Progenies of Viruo Prevalent Around 1981" Virology. 187. 748-759 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Ishiko et al: "Phylogenefically different strains of a variant of coxsockievirus A24 were repeatedly introduced but discontinued circulating in Japan" Arch.Virol. 126. 179-193 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 武田 直和,宮村 紀久子: "急性出血性結膜炎をおこす2つのエンテロウイルスの分子進化" 日本臨床. 50. 267-273 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 宮村 紀久子,山崎 修道: "エンテロウイルスの流行パターンと分子進化" 遺伝. 46. 36-44 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N.Takeda: "Evolutionary study on enterovirus" 70. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Ishiko: "Phylogenetic analysis of a coxsackievirus A24 variant: The most recent worldwide pandemic was caused by progenies of a virus prevalent around 1981" Virology. 187. 748-759 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Ishiko: "Phylogenetically different strains of a variant of coxsackievirus A24 were repeatedly introduced but discontinued circulating in Japan" Arch. Virol. 126. 179-193 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Miyamura: "Epidemiology and molecular evolution of enteroviruses" Genetics (in Japanese). 46. 36-44 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N.Takeda: "Molecular evolution of the two enteroviruses that caused acute hemorrhagic conjunctivitis (in Japanese)" Nippon Rinsho. 50. 267-273 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hiroaki Ishiko: "Phylogenetic Analysis of a Coxsackievirus A24 Variant:The Most Recent Worldwide Pandemic in as Caused by Progenies of a Virus Prevalent Around 1981" Virology. 187. 748-759 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroaki Ishiko: "Phylogenetically Different Sfrains of a Variant of Coxsackievirus A24 were Repeatedly Introduced but Discontinned in Japan" Archines of Virology. 126. 179-193 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 武田 直和: "急性出血性結膜炎をおこす2つのエンテロウイルスの分子進化ー RF-PCR法によるゲノム解析と分子疫学" 日本臨床 1992年特別号 感染症 遺伝子診断と分子疫学. 642. 267-273 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Hiroaki Ishiko: "Phylogenetic Analysis of a Coxsackievirus A24 Variant:The Most Recent Worldwide Pandemic was Caused by Progenies of a Virus Prevarent Around 1981" Virology. 187. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroaki Ishiko: "Phylogenetically Different Strains of a Variant of Coxsackierirus A24 were Pepeatedly Introduced but Discontinucd in Japan" Arch.Virol. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Naokazu Takeda: "Extremely High Transitional Nucleotide Substitution Rate and Greatly Restricted Amino Acid Changes in a Majov.Capsid Protein VP1 of EV70 during Ten Years Evolution in Natue" J.Virol.

    • Related Report
      1991 Annual Research Report
  • [Publications] 武田 直和: "Restricted amino acid changes in capsid protein of enterovirus 70 during the evolution" Virology.

    • Related Report
      1990 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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