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Molecular and immunological studies of cross-reactive idiotype-positive T cell antigen receptor

Research Project

Project/Area Number 02454188
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionKyoto University

Principal Investigator

KURIBAYASHI Kagemasa  Kyoto University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (10064578)

Co-Investigator(Kenkyū-buntansha) WATANABE Yoshihiko  Kyoto University, Faculty of Pharmacy, Associate Professor, 薬学部, 助教授 (90109075)
YAMAGISHI Hideo  Kyoto University, Faculty of Science, Associate Professor, 理学部, 助教授 (90025429)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordstumor immunity / CTL / TCR / idiotype somatic hypermutation / 体細胞突然変異 / 腫瘍免疫 / T細胞 / 抗原リセプタ- / 遺伝子 / イディオタイプ / 体細胞超突然変異
Research Abstract

A sonoclonal antibody (mAb Ng-127.) was raised against one of the CTL clone which specifically lysed tumor cells (FBL-3) induced by Friend virus in B6 mouse. This mAb detected a idiotype of TCR (1271d) composed of a specified combination of alpha and beta chains, that is, ValphalJalpha112-2/Vbeta10Dbeta2. IJbeta2.7.127Id bearing CTL appeared in appreciably high frequency in MLTC only after in vitro stimulation. All the CTL clones established from MLTC cells expressed TCR of the same composition as the immunizing one. Therefore, the epitope detected by this nAb was thought a cross-reactive idiotype expressed on FBL-3 specific CTL. These CTL clones were divided into two groups according to the blocking pattern of cytotoxic activities with sAb N9-127. The difference in the blocking susceptibility resided in a single amino acidsubstitution (Gly to Asp) in the D-J joint of bate chain. As compared with the germline sequence, the nucleotide exchange was observed in the Valpha chains of two out of eight clones examined, suggesting somatic point mutations. The nucleotide exchange was not seen in the V, beta.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Y.matsubayashi: "Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic anti-tumor responses." European Journal of Immunology. 20. 2095-2103 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Hirama: "Conserved V(D)J junctional sequence of cross-reactive cytotoxic T cell receptor idiotype and the effect of a single amino acid substitution." European Journal of Immunology. 21. 483-488 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Suzuki: "Expansion of murine T cells bearing a unique TCR β-chain in Friend virus-induced tumor in situ." Journal of Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中山 睿一: "腫瘍特異的キラ-T細胞におけるTCRイディオタイプの解析" 細胞工学. 9. 702-706 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 栗林 景容: "キラ-T細胞" 腫瘍免疫研究update.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 栗林 景容: "T細胞レパトマとがん" 細胞工学.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.kuribayashi: "Dominant idiotype in tumor-specific cytotoxic T-lymphocyte response.In"Idiotype Networks in Biology and Medicine",A.D.M.E.Osterhaus and F.G.C.M.UytdeHaag,edt." Elsevier Science Publishers B.V., 181-185 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Matsubayashi: "Participation of a dominant cytotoxic T cell population defined by amonoclonal antibody in syngeneic antiーtumor responses." European Journal of Immunology. 20. 2095- (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Hirama: "Conserved V(D)J junctional sequence of crossーreactive cytotoxic receptor idiotype and the effect of a single amino acid substitution." European Journal of Immunology. 21. 483- (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Suzuki: "Expansion of murine T cells bearing a unique TCR βーchain in Friend virusーinduced tumor in situ." Journal of Immunology.

    • Related Report
      1991 Annual Research Report
  • [Publications] 中山 睿一: "腫瘍特異的キラ-T細胞におけるTCRイディオタイプの解析" 細胞工学. 9. 702-706 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 栗林 景容: "キラ-T細胞" 腫瘍免疫研究update(中外医学社).

    • Related Report
      1991 Annual Research Report
  • [Publications] 栗林 景容: "T細胞レパトアとガン" 細胞工学.

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Kuribayashi: "Dominant idiotype in tumorーspecific cytotoxic Tーlymphocyte response.In “Idiotype Networks in Biology and Medicine",p181ー185,A.D.M.E.Osterhaus and F.G.C.M.Uytdehaag,edt." Elsevier Science Publishers B.V., (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Matsubayashi: "Participation of a dominant cytotoxic T cell population defined by a monoclonal antibody in syngeneic antiーtumor responses" European Journal of Immunology. 20. 2095-2103 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Hirama: "Conserved V(D)J junctional sequence of crossーreactive cytotoxic Tcell receptor idiotype and the effect of a single amino acid substitution" European Journal of Immunology. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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