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Expression and function of B cell specific MB-1 protein associated with IgM receptor

Research Project

Project/Area Number 02454192
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionTottori University (1992)
佐賀医科大学 (1990-1991)

Principal Investigator

SAKAGUCHI Nobuo  Tottori University, Faculty of Medicine Department of Immunology, Professor, 医学部, 教授 (70192086)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsIgM receptor / monoclonal Ab / signal transduction / pre-B cell / phosphorylation / lgMレセプター / IgMレセプタ- / モノクロ-ナル抗体 / preーB細胞 / pre B細胞
Research Abstract

We have previously identified a novel B cell specific gene named mb-1 that is expressed selectively in the restricted stage of B cell differentiation. In this project, we identified the B cell specific mb-1 gene product and found the association of the MB-1 protein with IgM receptor on the surface of B cells. To further characterize the expression and function of this molecule, we prepared rat monoclonal Ab against MB-1 protein on the cell surface. MB-1 is expressed already at the earliest stage in the B cell differentiation when IgM receptor ganes are with germ line configuration. Using mAbs, we demonstrated that MB-1 molecule itself is capable of mediating positive signals into B lineage cells in proliferation, increased Ca^<2+> concentration, and in tyrosne phosphorylation of intracellular proteins. These results suggested interesting but unknown functions of the IgR-associated molecules in the differentiation of early B lineage cells. We are currently started to analyze further function of MB-1 molecule in the experimental systems with DNA transfection, and gene targeting method.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Takai,T.: "Identification of IL-7-dependent bone marrow-derived Thy-1^-B2200^-lyphoid cell clones thet rearrange and express both Ig and T cell receptor genes." J.Immunol.148. 1329-1337 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kudo,A.: "The expression of the mouse VpreB/λ5 locus in transformed cell lines and tumors of the B lineage defferentiation pathway." Int.Immunol.4. 831-840 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Inui,S.: "Establishment of a murine pre-B cell clone dependent of interleukin-7 and stem cell factor." Immunology Letters. 34. 279-288 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Melchers,F.: "The surrogate light chain in B cell development." Immunology Today. 14. 60-68 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ishihara,K.: "Multiple B29 containing complexes on murine B lymphocytes. Common and stage restricted Ig-associated polypeptide chains." J.Immunol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuo,T.: "Crosslinking of B cell receptor-related MB-1 molecule induces the protein throsine phosphorylation in early B lineage cells." J.Immunol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi,N.: "Immunoglobulin receptor(IgR)-associated molecules." Advences in Immunology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takai,Y.: "Identification of IL-7-dependent bone marrow-derived Thy-1^-B220^-lymphoid cell clones that rearrange and express both Ig and T cell receptor genes." J.Immunol.148. 1329-1337 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kudo,A.: "The expression of the mouse VpreB/lambda5 locus in transformed cell lines and tumors of the B lineage differentiation pathway." Int.Immunol.4. 831-840 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Inui,S.: "Establishment of a murine pre-B cell clone dependent of interleukin-7 and stem cell factor." Immunology Letters. 34. 279-288 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Melchers,F.: "The surrogate light chain in B cell development." Immunology Today. 14. 60-68 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ishihara,K.: "Multiple B29 containing complexes on murine B lymphocytes. Common and stage restricted Ig-associated polypeptide chains." J.Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsuo,T.: "Crosslinking of B cell receptor-related MB-1 molecule induces the protein tyrosine phosphorylation in early B lineage cells." J.Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi,N.: "Immunoglobulin receptor (IgR)-associated molecules." Advances in Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takai,Y.: "Identification of IL-7-dependent bone marrow-derived Thy-1“B220"lymphoid cell clones thet rearrange and express both lg and Tcell receptor genes." J.Immunol.148. 1329-1337 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kudo,A.: "The expression of the mouse VpreB/λ5 locus in transformed cell lines and tumors of the B lineage differentiation Pathway." Int.Immunol.4. 831-840 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Inui,S.: "Establishment of a murine pre-B cell clone dependent of interleukin-7 and stem cell factor." Immunology Letters. 34. 279-288 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Melchers,F.: "The surrogate light chain in B cell development." Immunology Today. 14. 60-68 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ishihara,K.: "Multiple B29 containing complexes on murine B lymphocytes. Common and stage restricted lg-associated polypeptide chains." J.Immunol.

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsuo,T.: "Crosslinking of B cell receptor-related MB-1 molecule induces the protein tyrosine phosphorylation in early B lineage cells." J.Immunol.

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakaguchi,N.: "Immunoglobulin receptor (lgR)-associated molecules." Advances in Immunology.

    • Related Report
      1992 Annual Research Report
  • [Publications] Fujiwara,K.: "Sialadenitis in experimental graftーversusーhost disease:An animal model of Sjogren's syndrome." Laboratory Investigation. 65. 710-718 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 桑原 一彦: "リンパ球活性化のシグナル伝達路." 代謝.28. 367-372 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuo,T.: "Direct identification of the putative surface IgM receptorーassociated molecule encoded by murine B cellーspecific mbー1 gene." J.Immunol.146. 1584-1590 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松尾 達也: "免疫グロブリンレセプタ-とシグナル伝達。" 蛋白質核酸酵素.36. 662-672 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nomura,J.: "Signal transmission through the B cellーspecific MBー1 molecule at the preーB cell stage." Int.Immunol.3. 117-126 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 野村 純: "B細胞特異的遺伝子mbー1とそのB細胞活性化における役割." 細胞工学.10. 38-46 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 阪口 薫雄: "B細胞特異的遺伝子発現;その発現および機能." Medical Immunology.21. 215-219 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 阪口 薫雄: "B細胞活性化の分子機構." Medical Immunology.21. 221-238 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 阪口 薫雄: "Ig関連分子." Medical Immunology.22. 23-34 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松尾 達也: "IgMレセプタ-結合MBー1分子の構造と機能." Medical Immunology.22. 25-34 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takai,Y.: "Identification of ILー7ーdependent bone marrowーderived Thyー1ーB220ーlymphoid cell clones that rearrange and express both Ig and T cell recceptor genes." J.Immunol.

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Sakaguchi: "B lymphocyte lineageーrestricted expression of mbー1,a gene with CD3ーlike structural properties." EMBO J.7. 3457-3464 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sーi.Kashiwamura: "Structure of the murine mbー1 gene encoding a putative sIgMーassociated molecule." J.Innunol.145. 337-343 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Nomura: "Signal transmission through the B cellーspecific MBー1 molecule at the preーB cell stage." Int.Immunol.3. 117-126 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Matsuo: "Direct identification of the putative surface IgM receptorーassociated molecule encoded by murine B cellーspecific mbー1 gene." J.Immunol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 松尾 達也: "B細胞機能分子" Annual Review免疫1990. 102-111 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 野村 純: "膜結合型免疫グロブリン" 日本臨牀. 48. 239-244 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小山 孝則: "B細胞におけるシグナル伝達分子" 代謝臨時増刊号「先端の医生物学とバイオサイエンス」. 27. 131-136 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 松尾 達也: "免疫グロブリンレセプタ-とシグナル伝達" 蛋白質核酸酵素. 36. 662-672 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 野村 純: "B細胞特異的遺伝子mbー1とそのB細胞活性化における役割" 細胞工学. 10. 38-46 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 阪口 薫雄: "B細胞特異的遺伝子発現:その発現及び機能" Medical Immunology(国際医書出版).

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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