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Exposure Level Monitor of Carcinogenic Tryptophan Pyrolysis Products in Humans.

Research Project

Project/Area Number 02454198
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionUniversity of Tokyo

Principal Investigator

MANABE Shigeo  Univ. of Tokyo, Dept. of Hygiene, Associate professor, 医学部(医), 助教授 (90165928)

Co-Investigator(Kenkyū-buntansha) 長橋 捷  東京大学, 医学部・医学科, 助手 (90009994)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsCarcinogen / Mutagen / Tryptophan Pyrolysates / Exposure Monitor / 変異原性
Research Abstract

In order to determine a suitable indicator for estimating the exposure levels of the environmental carcinogen, carcinogenic tryptophan pyrolysis products, the levels of these carcinogens in plasma, platelets and red blood cells (RBC) were monitored for 12 weeks by a high-performance liquid chromatography method after these carcinogens were administered as single oral doses (2-20 mg) to rabbits. The results indicate that carcinogenic tryptophan pyrolysis products in RBC that bound covalently to the hemoglobin is the most suitable for monitoring the long-term exposure levels. Moreover, it has been demonstrated that these carcinogens are easily released from hemoglobin adducts by acid (0.5N HCI) treatment at 60゚C. Based on these findings obtained in animal experiments, the method is applied for monitoring the exposure levels of carcinogenic tryptophan pyrolysis products in humans. The total number of subjects studied was 16, of whom 8 were healthy normal subjects and 8 were uremic patients treated with hemodialysis. Levels of carcinogenic tryptophan pyrolysis products ih plasma and RBC of these subjects were determined. In both uremic patients and normal subjects, these carcinogens have been detected in RBC as well as plasma. Moreover, these carcinogen levels in RBC (per hemoglobin) were significantly elevated in uremic patients. These results support the idea that these carcinogens. in RBC are suitable for monitoring exposure levels in humans.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] S.Manabe and O.Wada: "Carcinogenic tryptophan pyrolysis products in the environment" J.Toxic.Sci. 16(Suppl). 63-72 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Manabe et al.: "Detection of carcinogenic amino-a-carbolines and amino-r-carbolines in diesel-exhaust particles." Environ.Pollution. 70. 255-265 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Manabe et al.: "Pharmaco-toxicological aspects of carcinogenic tryptophan pyrolysis products in the environment." J.Envion.Sci.&Health. A26. 1449-1469 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Manabe et al.: "Carcinogenic amino-a-carbolines and amino-r-carbolines in cooked foods and soy sauce." Environ.Sci.1. 11-19 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Manabe et al.: "Detection of a carcinogen,2-amino-1-methy1-6-phenyl-imidazo|4,5-b|pyridine(PhIP)in cigarette smoke" Carcinogenesis. 12. 1945-1947 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Manabe et al.: "Elevation of levels of carcinogenic tryptophan pyrolysis products in plasma and red blood cells of patients with uremia." Clin.Nephrol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Manabe and O. Wada: "Carcinogenic Tryptophan Pyrolysis Products in the Environment." J. Toxic. Sci.16(Suppl). 63-72 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Manabe et al.: "Detection of Carcinogenic Amino-a-carbolines and Amino-r-Carbollines in Diesel-exhaust Particles." Environ. Pollution. 70. 255-265 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Manabe et al.: "Pharmaco-Toxicological Aspects of Carcinogenic Tryptophan Pyrolysis Products in the Environment." J. Environ. Sci, & Health. A26. 1449-1469 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Manabe et al.: "Carcinogenic Amino-a-carbolines and Amino-r-carbolines in Cooked Foods and Soy Sauce." Environ. Sci.1. 11-19 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Manabe et al.: "Detection of a Carcinogen, 2-amino-1-methyl-6-phenylimidazo |4, 5-b|pyridine (PhIP) in Cigarette Smoke." Carcinogenesis. 12. 1945-1947 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Manabe et al.: "Elevation of Levels of Carcinogenic Tryptophan Pyrolysis Products in Plasma and Red Blood Cells of Patients with Uremia." Clin. Nephrol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S.Manabe and O.Wada: "Carcinogenic tryptophan pyrolysis products in the environment." J.Toxic.Sci.16(Suppl). 63-72 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Manabe et al.: "Detection of carcinogenic aminoーaーcarbolines and aminoーrーcarbolines in dieselーexhaust particles." Environ.Pollution. 70. 255-265 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Manabe et al.: "Pharmacoーtoxicological aspects of carcinogenic tryptophan pyrolysis products in the environment." J.Environ.Sci.& Health. A26. 1449-1469 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Manabe et al.: "Carcinogenic aminoーaーcarbolines and ainoーrーcarbolines in cooked foods and soy sauce." Environ.Sci.1. 11-19 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Manabe et al.: "Detection of a carcinogen,2ーaminoー1ーmethy1ー6ーphenylーimidazo |4,5ーb|pyridine(PhIP)in cigarette smoke." Carcinogenesis. 12. 1945-1947 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Manabe et al.: "Elevation of levels of carcinogenic tryptophan pyrolysis products in plasma and red blood cells of patients with uremia." Clin.Nephrol.

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Manabe et al.: "Simultaneous determination of aminoーaーcarbolines and aminoーrーcarbolines in cigarette smoke condensate by highーperformance liquid chromatography." J.Chromatography. 529. 125-133 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Manabe & O.Wada: "Carcinogenic tryptophan pyrolysis products in cigarette smoke condensate and cigarette smokeーpolluted indoor air" Environ.Pollution. 64. 121-132 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Manabe & O.Wada: "Identification of carcinogenic tryptophan pyrolysis products in human bile by highーperformance liquid chromatography." Environ.Molecular Mutagen.15. 229-235 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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