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Association of viral infection to production of autoantibodies-Anti-U1 RNP antibody and retro virus infection-

Research Project

Project/Area Number 02454223
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionJuntendo University School of Medicine

Principal Investigator

HIROSE Shun-ichi  Juntendo Univ., School of Medicine, Professor, 医学部, 教授 (70010147)

Co-Investigator(Kenkyū-buntansha) 山中 健次郎  順天堂大学, 医学部, 助手 (60230504)
高崎 芳成  順天堂大学, 医学部, 講師 (80154772)
TAKASAKI Y  Juntendo Univ., School of Medicine Assistant professor
YAMANAKA K  Juntendo Univ., School of Medicine Instructor
大道寺 英幸  順天堂大学, 医学部, 助手 (80207266)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsAutoantibod / Autoantigen / U1 RNP / retro virus / MCTD / SLE / HIV / 抗核抗体 / 抗U1 RNP抗体 / AIDS
Research Abstract

Purification of U1 RNP antigen was performed and an ELISA for anti-U1 RNP antibodies was established. Using this system, cross-reactivity between U1 RNP and HIV associated antigens was studied. Twenty-five %of sera positive for anti-HIV antibodies reacted with this system in addition to anti-U1 RNP positive sera from patients with SLE and MCTD. This reaction was specifically inhibited by the U1 RNP antigen. In addition, the reactions of those sera in passive hemmagulutination test (PA) and immunoblot assay (IB) for HIV were also specifically inhibited by the U1 RNP antigen. Five % of anti-U1 RNP positive sera from MCTD reacted with HIV associated antigens in PA and were also reactive with p24, 31 and / or 51 in IB. Those results suggested the cross-reactivity between HIV associated antigens and U1 RNP. To study their relationship further, an U1 RNP synthetic peptide that has an amino acid sequence homologous to retrovirus associated antigen, p30^<gag> was prepared and used in ELISA. This system specifically reacted with sera from patients with MCTD and was thought to be useful for the diagnosis of MCTD. In contrast, none of sera with anti-HIV antibodies reacted with 68SP. But all those anti-HIV positive sear reacted with U1 RNP 68kD polypeptide in IB. Those results suggest that U1 RNP and HIV associated antigen share the cross-reactive epitope which is different from that homologous to p30^<gag> on 68 kD polypeptide, and the viral infection is associated with the production of antibodies to U1 RNP.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Yasuma,M.,Takasaki,Y.,Matsumoto,K.,Kodama,A.,Hashimoto,H.and Hirose,S.: "Clinical signiticance of IgG anti-Smantibodies in patients with systemic lupus erythematosus." J.Rheumatol.17. 469-490 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 大道寺 英幸: "Immunoblotting 法を用いた抗U1 RNPおよび抗Sm抗体の補体結合性の検討" 日臨免会誌. 14. 395-402 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nikaido,T.,Shimada,K.,Shibata,M.,Hata,M.,Sakamoto,M.,Takasaki,Y.,Sato,C.,Takanashi,T.and Nichida,Y.: "Cloning and nucleotide seguence of cDNAtor Kiantigen,a highly conserved nuclear protein detected with seratrom patients with systemic lupus erythematosus." Clin.exp.Immunol.79. 209-214 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tokano,Y.,Yasuma,M.,Harada,S.,Takasaki,Y.,Hashimoto,H.,Okumura,K.and Hirose,S.: "Clinical significance of IgG subclass of anti-Sm and U1 RNP antibodies in patients with systemic lupuserythematosus and mixed connective tissue disease." J.Clin.Immunol. 11. 317-319 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takasaki,Y.,Yamanaka,K.,Shimada,K.,Shibata,M.,Hashimoto,H.and Hirose,S.: "An epitope on Ki antigen revognized by autoantibodies from lupus patients is homologous to SV40 large T antigen nuclear localization sign." Moled.Biol.Rept.15. 216 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamanaka,K.,Takasaki,Y.,Nishida,Y.,Shimada,K.,Shibata,M.and Hashimoto,H.: "Detection and guantification of anti-Ki antibodies by enzyne-linked immunosor bent assay using recombinant Ki antigen" Arthritis Rheum.35. 667-671 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yasuma,M., Takasaki,Y., Matsumoto,K., Kodama,A., Ha-shimoto,H. and Hirose,S.: "Clinical significance of IgG anti-Sm antibodies in patients with systemic lupus erythematosus." J. Rheumatol.17. 469-490 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nikaido,T., Shimada,K., Shibata,M., Hata,M., Sakamoto,M., Takasaki,Y., Sato,C., Takahashi,T. and Nishida,Y.: "Cloning and nucleotidesequence of cDNA for Ki antigen, a highly conserved nuclear protein detected with sera from patients with systemic lupus erythematosus." Clin.exp.Immunol.79. 209-214 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tokano,Y., Yasuma,M., Harada,S., Takasaki,Y., Hashimo-to,H., Okumura,K. and Hirose,S.: "Clinical significance of IgG subclass of anti-Sm and U1 RNP anti-bodies in patients with systemic lupus erythematosus and mixed connective tissue disease." J. Clin. Immunol.11. 317-319 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takasaki,Y., Yamanaka,K., Shimada,K., Shibata,M., Hashimoto,H. and Hirose,S.: "An epitope on Ki antigen recognized by autoantibodies from lupus patients is homologous to SV40 large T antigen nuclear localization sign." Molec. Biol. Rep.15. 216 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yamanaka,K., Takasaki,Y., Nishida,Y., Shimada,K., Shibata,M. and Hashimoto,H.: "Detection and quantification of anti-Ki antibodies by enzyme-linked immunosorbent assay using recombinant Ki antigen." Arthritis Rheum.35. 66-7-671 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 高崎 芳成、山中 健次郎、浅野 正直、川口 里江子、宮川 薫、矢野 哲郎、橋本 博史、廣瀬 俊一: "リコンビナント抗原を用いた抗U1RNP抗体の測定" 厚生省特定疾患混合性結合組織病調査研究班. 23-25 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Yamanaka,K.,Takasaki,Y.,Nishida,Y.,Shimada,K.,Shibata,M.,and Hashimoto,H.: "Detection and quantification of anti-Ki antibodies by enzyme-linked immunoscrbent assay using recombinant Ki antigen." Arthritis Rheum.35. 667-671 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Tokano,Y,Yasuma,M,Harada,S,Takasaki,Y,Hashimoto,H,Okumura,K and Hirose,S.: "Clinical significance of IgG subclasses of antiーSm and Ul bibonuーCleprotein antibodies in patients with systemic lupus erythematosus and mixed connective tissue disease." J.Clin.Immunol.11. 317-325 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Miyajima,H,Hirano,T,Hirose,S.and Okumura,K.: "Supression by ILー2 of IgE production by B cells stimulated by ILー4." J.Immunol.135. 184-194 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hashimoto,H,Tuda,H,Kanai,Y,Yokoyama,M,Mokuno,C.and Hirose,S.: "Selection removal of antiーDNA and antiーCL by adsorbent plasmapheresis using dextran sulfate columns in patients with SLE." J.Rheumatol.18. 545-551 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Negishi,K,Stell,K.W.,Teranishi,T,karhanis,A,Owusuyaw,V.and Takasaki,Y.: "Induction of proliferating cell nuclear antigen(PCNA)ーImmunoreactive cells in goldfish retina following intravitreal injection with 6ーhydroxydopamin." Cell.Molec.Neurobiol.11. 639-659 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Haneda,H,Katabami,M,Miyamoto,H,Isobe,H,Shimizu,T,Ishiguro,A,Moriuchi,T,Takasaki,Y.and kawakami,Y.: "Therelationship of the proliferating cell nuclear antigen protein to cisーdiamminedichroplatinum(II)resistance of a murine leukemia cell line P388/CDDP." Oncology. 48. 234-238 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nakamura,T,Ebihara,I,Takasaki,Y,Tomino,Y and Koide,H.: "Increased expression of proliferating cell nuclear antigen mRNA in peripheral blood mononuclear cells from patients with IgA nephropathy." Ame.J.Med.Sci.302. 214-219 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 廣瀬 俊一(分担執筆): "臨床医のための概念の整理" 真興交易(株)出版部, 326 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 廣瀬 俊一(分担執筆): "内科学" 朝倉書店, 1724 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Takasaki,M.Ohgaki,A.Kodama,K.Ogata,H.Hashimoto,T.Shirai&S.Hirose: "A sandwith type enzymeーlinkedーimmunosorbentーassay for proliferating cell nuclear antigen(PCNA)/cyclin using monoclonal antibodies." J.Immunol.Meth.132. 227-237 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Yasuma,Y.Takasaki,K.Matsumoto,A.Kodama,H.Hashimoto & S.Hirose: "Clinical significance of I_gG antiーSm antibodies in patients with systemic 11upus erythematosus." J.Rheumatol.17. 469-475 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.Murashima,Y.Takasaki,M.Ohgaki,H.Hashimoto,T.Shirai & Hirose: "Activated periferal blood mononuclear cells detected by murine monoclonal antibodies to proliferating cell nuclear antigen in active lupus patients." J.Clin.Immunol.10. 28-37 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Hishikawa,Y.Tokano,I.Sekigawa,S.Ando,Y.Takasaki,H.Hashimoto,S.Hirose et al: "HLAーDP^+Tcells and deficient interleukinー2production in patients with systemic lupus erythematosus." Clin.Immunol.Immunopathol.55. 285-296 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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