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Study on the effects of hepatites B virus X-gene expression on hepato-carcinogenesis.

Research Project

Project/Area Number 02454232
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

HAYASHI Norio  Osaka University Medical School, Lecturer, 医学部, 講師 (00144478)

Co-Investigator(Kenkyū-buntansha) KATAYAMA Kazuhiro  Osaka University Hospital, Staff, 医学部附属病院, 医員
KASAHARA Akinori  Osaka University Medical School, Assistant, 医学部, 助手 (70214286)
佐々木 裕  大阪大学, 医学部, 助手
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1991: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1990: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordshepatitis B Virus / x-gene / x-protein / oncogene / HCC / carcinogenesis / replication / HLA class I Antigen / hepatitis B vins / Hcc / integration / B型肝炎ウイルス / X遺伝子 / X蛋白 / X抗体 / 癌遺伝子
Research Abstract

In patients with type B chronic hepatitis, X protein was expressed in cytoplasm of hepatocyte. The distribution of X protein-positive hepatocytes was correlated with that of HBcAg-positive ones. Furthermore, serum HBV DNA levels of X protein-positive patients were significantly higher than those of X protein-negative ones, suggesting that X protein which was expressed in liver tissue might enhance the replication of HBV in vivo. When we examined three cases of patients with type B hepatocellular carcinoma (HCC), X protein was not expressed in cancerous region of all three patients, but was expressed in non-cancerous region of two of three patients. These findings suggested that X protein had some relations to a early stage of hapato-carcinogenesis. However, it was unlikely that X protein might function to maintain HCC. Furthermore, in patients with type B chronic hepatitis and liver cirrhosis, the frequency of the expression of c-myc was significantly higher in patients with X protein … More than that without X protein. However, the distribution of X protein was different from that of c-myc. Therfore, at present, it is unclear whether, in patients with type B chronic liver disease, the expression of oncogenes such as c-myc and c-Ha-ras were directly induced by X protein or indirectly induced as a result of inflammation. From this point of view, further study is necessary.
Moreover, we examined a relationship between HBV replication and the ability of HBV-infected hepatocytes to express HLA antigen, using HB611 cell line which was developed from HUH6 cell line by integrating HBV-DNA. In this cell line, X protein was observed in peri-nuclear cytoplasm. Pretreatment of this HB611 with acyclocir to inhibit HBV replication increased the expression of HLA class I antigen on HB611, suggesting that HBV replication might suppress the expression of HLA antigen. Decreased expression of HLA antigen might impair the ability of immunosurveillance system to recognize antigens and is considered to have some relations to malignancy. Further study is necessary to be concluded. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Haruna Y,et al: "Expression of X protein and hepatitis B vines veplication in ehronic hepatitis" Hepotology. 13. 417-421 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takehara T,et al.: "Enhanced expression of HLA class I by inhibited replication of hepatitis B vines." Journal of Hepatology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuki N,et al.: "Detection of antibodies againct the polymerase gene product in hepatitis B virus infection" Hepatology. 12. 193-198 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Towata T,et al.: "Signal trans duction pathways in the induction of HLA class I antigen expression on HUH6 cells by interferon-gamma" Biochem Biophy Res Comm.177. 610-617 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 三田 英治,他: "慢性肝疾患におけるcーmycおよびCーHaーras発現に関する検討" 肝臓. 31. 118-122 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 結城 暢一,他: "HCV抗体陽性慢性肝疾患におけるHBV関連マ-カ-の検討" 日消誌. 87. 2466-2472 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Haruna Y, et al.: "Expression of X protein and hepatitis B virus replication in chronic hepatitis." Hepatology. 13. 417-421 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takehara T, et al.: "Enhanced expression of HLA class I by inhibited replication of hepatitis B virus." J Hepatology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuki N, et al.: "Detection of antibodies against the the polymerase gene product in hepatitis B virus infection." Hepatology. 12. 193-198 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Towata T, et al.: "Signal transduction pathways in the induction of HLA class I antigen expression on HUH6 cells by interferon-gamma." Biochem Biophy Res Comm. 177. 610-617 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Haruna.Y,et al.: "Expression of X protein and hepititio B vins replication in Chronic hepititis" Hepctology. 13. 417-421 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takehara et al.: "Enhamced expression of HLA class I by inbribited replication of hepati tis B vins" Journal of Hepsto logy. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Towata,et al: "Signal trams duction pathways in the induction of HLA class I antigen expression on HOH6 cells by inter feionーgamma." Btochem Biophy Res Comm. 177. 610-617 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yuki N,et al.: "Detection of antibodies agaiust the polymerase gene product in hepatitis B virus infection." Hepatology. 12. 193-198 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Haruna Y,et al.: "Expression of X protein and hepatitis B virus replication in chronic hipatitis." Hepatology. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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