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Molecular and Biological Study on Roles of Colony-Stimulating Factors in the Formation of Granulomatous Lung Lesions.

Research Project

Project/Area Number 02454238
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionHokkaido University

Principal Investigator

YAMAGUCHI Etsuro  Hokaido University Medical Hospital, Instructor, 医学部附属病院, 助手 (10201831)

Co-Investigator(Kenkyū-buntansha) KUZUMAKI Noboru  Hokkaido University, School of Medicine, Professor, 医学部, 教授 (80091445)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥5,800,000 (Direct Cost: ¥5,800,000)
KeywordsGranulocyte-macrophage colony-stimulating factor (GM-CSF) / Interleukin-3 (IL-3) / Macrophage colony-stimulating factor (M-CSF) / Reverse transcription-polymerase chain reaction (RT-PCR) / Sarcoidosis / Bronchoalveolar lavage (BAL) / マクロファ-ジコロニ-刺激因子 / 逆転写ポルメア-ゼチェインリアクション / GMーCSF / ILー3 / MーCSF / PCR / BAL
Research Abstract

Pulmonary sarcoidosis is characterized by the accumulation of a great number of alveolar macrophages at local milieu of the lung. In order to investigate its mechanism, We aimed to examine the expression of mRNA of several colony-stimulating factors (CSFs). We extracted RNA from inflammatory cells obtained by bronchoalveolar lavage, which were composed mainly of alveolar macrophages and lymphocytes. mRNA of interest was detected by reverse transcription-polymerase chain reaction (RT-PCR) method using synthesized primers of macrophage-CSF (M-CSF), interleukin-3 (IL-3), and granulocytemacrophage-CSF (GM-CSF).
M-CSF mRNA was detected in all normal subjects, patients with pulmonary sarcoidosis, and patients with farmer's lung disease. Meanwhile, IL-3 mRNA was detected none of them. GM-CSF mRNA was detected in 15 of 20 patients with pulmonary sarcoidosis, whereas it was expressed in none of normal subjects and patients with farmer's lung disease. GM-CSF is assumed to contribute the accumulat … More ion of alveolar macrophages by activation, differentiation, and proliferation of monocytes. As it also has a fusing effect of alveolar macrophages, it may be involved in the formation of multinucleated giant cells within the granulomas.
It is noteworthy that results of the expression of GM-CSF mRNA was contrasting between pulmonary sarcoidosis and farmer's lung disease, suggesting different pathogenetic mechanisms are working in these two granulomatous lung diseases.
We also examined the relationship between the expression of GM-CSF mRNA and clinical significance in pulmonary sarcoidosis. Patients with mRNA expression had significantly higher proportion of bronchoalveolar lavage lymphocytes, CD4/8 ratio, and serum ACE levels than those without the expression. Retrospective study from the time when mRNA was examined revealed association between mRNA expression and unchanged or worse clinical course judged by chest X-ray films. Prospective study gave similar results, thus demonstrating substantial role of GM-CSF in the pathogenetic mechanism of pulmonary sarcoidosis. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Itoh A,Yamaguchi E,Kuzunoki N,Okazaki N,Furuya K,Abe S,Kawakami T.: "Expression of granulocyte-macrophage colony-stimulating factor mRNA by inflammatory cells in the sarcoed lung." An J Respin Cell Mol Biol. 3. 245-249 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 伊藤 昭英,山口 悦郎,古家 乾,桧沢 伸之,阿部 庄ご,川上 義和: "サルコイド-シス患者BAL細胞によるGMーCSFmRNA発現とその臨床的意義" サルコイド-シス学会雑誌. 10. 93-94 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 山口 悦郎,川上 義和: "BALで何がわかるか、病態生理学的意義ーDNAのレベルからー" 気管支学. 14. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 山口 悦郎,川上 義和(分担): "Annual Review,呼吸器1992" 中外医学社, 218(14) (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akihide Itoh, Etsuro Yamaguchi, Noboru Kuzumaki Ken Furuya, Nozomu Okazaki, Shosaku Abe, Yoshikazu Kawakami: "Expression of granulocyte-macrophage colony-stimulating factor mRNA by inflammatory cells in the sarcoid lung." Am J Respir Cell Mol Biol. 3-3. 245-249 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akihide Itoh, Etsuro Yamaguchi, Ken Furuya, Nobuyuki Hizawa, Shosaku Abe, Yoshikazu Kawakami: "Expression of GM-CSF mRNA by BAL cells from sarcoid patients and its clinical significance." Jpn J Sarcoidosis. 10. 93-94 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Etsuro Yamaguchi, Yoshikazu Kawakami: "What can we know by BAL?-from DNA level-" Bronchology. 14. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Etsuro Yamaguchi, Yoshikazu Kawakami: "Cytokine" Annual Review Respiratiory Organ 1992. Chugai Igaku. 64-77 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 山口 悦郎,川上 義和: "BALで何がわかるか、病態生理学的意義ーDNAのレベルからー" 気管支学. 14. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 伊藤 昭英,山口 悦郎,古家 乾,桧沢 伸之,阿部 庄作,川上 義和: "サルコイド-シス患者BAL細胞によるGMーCSFmRNA発現とその臨床的意義" サルコイド-シス学会雑誌. 10. 93-94 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 山口 悦郎,川上 義和(分担): "Annual Revies.呼吸器 1992" 中外医学社, 218(14) (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Itoh A,Yamaguchi E,Kuzumaki N,Okazaki N,Furuya K,Abe S,Kawakami Y.: "Expression of granulocyteーmacrophage colonyーstimulating factor mRNA by inflammatory cells in the sarcoid lung." Am J Respir Cell Mol Biol. 3. 245-249 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 伊藤 昭英,山口 悦郎,古家 乾,檜沢 伸之,阿部 庄作,川上 義和: "サルコイド-シス患者BAL細胞によるGMーCSF mRNA発現とその臨床的意義" サルコイド-シス学会雑誌. 10. (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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