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Interaction of Angiotensin II and kinin on the formation of atherosclerotic lesion

Research Project

Project/Area Number 02454264
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionFukuoka University

Principal Investigator

ARAKAWA Kikuo  Fukuoka University School of Medicine, Professor, 医学部, 教授 (90078783)

Co-Investigator(Kenkyū-buntansha) SASAGURI Manabu  Fukuoka University School of Medicine, Lecturer, 医学部, 講師 (00178675)
IDEISHI Munehito  Fukuoka University School of Medicine, Lecturer, 医学部, 講師 (20131807)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsAngiotensin / bradykinin / endothelial cell / smooth muscle cell / chymostatin / 増殖因子 / 平滑筋細胞 / アンジオテンシンII
Research Abstract

1. Vascular endothelial cells and smooth muscle cells were obtained from porcine aorta. Effects of angiotensin II and bradykinin on intracellular Ca concentration, expression of protooncogene c-fos and proliferations of these cells were examined.
(1)Smooth muscle cell proliferation measured by DNA systhesis were not altered by the addition of angiotensin II or bradykinin. In addition, angiotensin converting enzyme inhibitor and angiotensin antagonist were also not effective. On the contrary, Co-culture with confluent endothelial cells or the addition of post culture medium of endothelial cells stimulated the growth of smooth muscle cells. The endothelial cell-derived smooth muscle cellgrowth factor was inactivated totally by heat treatment and partially by trypsinization. The molecular weight of the factor was estimated as 50-100KDa by a filtration method. These suggested the factor was distinct from known growth factors PDGF or FGF.
(2)Intra cellular free Ca concentration was measure by Fura-2 method. Both angiotenisn II and bradykinin raised intracellular free Ca concentration of smooth muscle cells. Repetitive application of angiotensin II showed well known tachyphylaxis. However, pretreatment with bradykinin did not interfere with the effect of angiotensin II. Endothelial cells in this experiment did not respond to bradykinin.
(3)Protooncogene c-fos expression was determined by Nortehern blot analysis. Both angiotenisn II and bradykinin stimulated c-fos expression.
2. Clinical study on the interaction of angiotensin II and bradykinin We have previously reported the alternate angiotensin II forming pathway, kinin-tensisn system. In which, serine protease such as trypsin and kallikrein forms both kinins in an alkaline condition and angiotensin II in an acidic condition. The operation of the pathway in humans has not confirmed.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] M.Sasguri,M.M.Ideishi M.Ikeda,K.Arakawa: "Inhibitory effects of kinins on angiotenisn I conversion in the local circulation" Clinical and Experimental Hypertension. A12. 551-569 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Ideishi,M.Ikeda,K.Arakawa: "Angiotensin-converting activity of tissue kallikrein" Nephron. 55. 62-64 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Ideishi,M.Sasaguri,M.Ikeda,K.Arakawa: "Substrate dependent angiotensin II formation in the peripheral circulation" Life Sciences. 46. 335-341 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Ideishi,K.Noda,M.Sasaguri,M.IKeda,K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" Artery. 20. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Noda,M.Sasaguri,M.Ideishi,M.Ikeda,K.Arakawa: "Role of locally formed angiotensin II and bradykinin in the reduction of myocardial infarct size in dogsc" Cardiovascular Research. 27. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Urabe,M.Ideishi,M.Sasaguri,M.Ikeda,K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" American Journal of Cardiology. 72. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Sasaguri, M. Ideishi, M. Ikeda, K. Arakawa: "Inhibitory effects of kinins on angiotensin I conversion in the local circulation" Clinical and Experimental Hypertension. A12. 551-569 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Ideishi, M. Ikeda, K. Arakawa: "Angiotensin-converting activity of tissue kallikrein" Nephron. 5. 62-64 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M. Ideishi, M. Sasaguri, M. Ikeda, K. Arakawa: "Substrate dependent angiotensin II formation in the peripheral circulation" Life Sciences. 46. 335-341 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Ideishi, K. Noda, M. Sasaguri, M. Ikeda, K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" Artery. 20. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Noda, M. Sasaguri, M. Ideishi, M. Ikeda, K. Arakawa: "Role of locally formed angiotensin II and bradykinin in the reduction of myocardial infarct size in dogsc" Cardiovascular Research. 27. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y. Urabe, M.Ideishi, M. Sasaguri, M. Ikeda, K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" American Journal of Cardiology. 72. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Noda,M.Sasaguri,M.Ideishi,M.Ikeda,K.Arakawa: "Role of locally formed angiotensin II and bradykinin in the reduction of myocardial infarct size in dogs" Cardiovascular Research. 27. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Urabe,M.Ideishi,M.Sasaguri,M.Ikeda,K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" American Journal of Cardiology. 72. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Ideishi,K.Noda,M.Sasaguri,M.Ikeda,K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" Artery. 20. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.Ideishi,K.Noda,M.Sasaguri,M.Ikeda,K.Arakawa: "Angiotensin II forming activity of vascular endothelial and smooth muscle cells" Life Sciences.

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Noda,M.Ikeda,M.Ideishi,M.Sasaguri,K.Arakawa: "Role of locally formed angiotensin II and in the ischemic dog heart" Cardiovascular Research.

    • Related Report
      1991 Annual Research Report
  • [Publications] Sasaguri M et al.: "Inhibitory effects of kinins on angiotensin I conversion in the local circulation" Clinical and Experimental Hypertension. A12. 551-569 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ideishi M et al.: "Substrateーdependent angiotensin II formation in the peripheral circulation" Life Sciences. 46. 335-341 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ideishi M et al.: "Angiotensinーconverting activity of tissue kallidrein" Nephron. 55. 62-64 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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