| Project/Area Number |
02454276
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Aichi Cancer Center Research Institute |
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Principal Investigator |
KOIWAI Osamu Aichi Cancer Center Research Institute, Biochemistry, Senior researcher., 研究所・生化学部, 主任研究員 (50132923)
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| Co-Investigator(Kenkyū-buntansha) |
HANAOKA Kazunori National Institute of Neuroscience, National Center of Neurology and Psychiatry., モデル動物開発部, 室長 (40189577)
TADA Mariko Same Institute as head investigator, Biochemistry. Section head., 研究所・分子生物学研究室・生化学部, 室長 (90073113)
NAKAMURA Hiromu Same Institute as head investigator, Molecular biology. Section head., 研究所・分子生物学研究室, 室長 (30109938)
NAKASU Akira Same Institute as head investigator, Molecular biology. Senior researcher., 研究所・分子生物学研究室, 研究員 (50198107)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | T-cell Receptor / Recombination / Molecular Biology |
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| Research Abstract |
We are investigating the regulation of the gene expression for terminal deoxy nucleotidyltransferase(TdT)by constructing the transgenic mice. We have already isolated the upstream region(2000 bp)of the cap site of human TdT gene and determined that the 1000 bp in the region plays an important role for the TdT expression in in vitro system. We first asked whether this region plays a crutial role as the promoter of TdT gene in in vivo. It is reasonable to speculate that the region promotes the gene expression of TdT in B-cells, because the enhancer elements involved in the specific expression in the B-cells are detected in the region. On the contrary, we could not detect the T-cell specific enhancer elements in the region. In our experiment, we will be able to clarify if this region also controles the TdT gene expression in T-cells. To attain this purpose, we constructed the expression vector in which the promoter combined with the reporter gene of beta-galactosidase. 3' Non-coding region of mouse TdT cDNA was inserted at the downstream of the fused gene. After large scale purification of the plasmid, the DNA fragment digested with endonuclease ScaI and PstI was introduced into the mouse embryo. We are now producing the transgenic mice and analysing the gene expression of TdT.
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