| Project/Area Number |
02454290
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Psychiatric science
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| Research Institution | Tohoku University |
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Principal Investigator |
MIZUGAKI Michinao Tohoku University, Hospital, Professor, 医学部附属病院, 教授 (60004595)
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| Co-Investigator(Kenkyū-buntansha) |
HISHINUMA Takanori Tohoku University, Hospital, Lecturer, 医学部附属病院, 講師 (20199003)
EDO Kiyoto Fukushima Prefectural College of Medicine, Hospital, Assistant Professor, 助教授 (40125505)
SATO Mitsumoto Tohoku University, School of Medicine, Professor, 医学部, 教授 (70033321)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | cocaine / drug / positron / addiction / biodistribution / sensitization |
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| Research Abstract |
A tropane type alkaloid, ^<11>C-labeled cocaine was synthesized by N-methylation of norcocaine with ^<11>CH_3I to the assist in the imaging the variety of local distribution by positron emission tomography (PET). The radiochemical yield and the radiochemical purity after purification of ^<11>C-cocaine by HPLC were 58% and > 99% at a specific activity of 22 Ci/mmol. The organ- and regional-distribution of ^<11>C-cocaine was investigated in mice at various times after i. v. injection and we obtained the results of the distribution of ^<11>C-cocaine as follows.
1) The accumulation of radioactivity was in the lung, the kidney, the liver and the small intestine.
2) No regional differences was observed in the ^<11>C-cocaine uptake in the brain, and the radioactivity level reached the maximum at 5 min. in non-treated mice.
3) The sensitization to cocaine delayed the clearance of ^<11>C-cocaine from the brain, the kidney and the stomach.
4) In the sensitized model of cocaine, the peaks of ^<11>C-cocainc uptake shifted to 10-15min. after the i. v. injection in most brain regions.
5) The pretreatment of cold cocaine did not show the difference of the uptake-pattern of ^<11>C-cocaine in brain.
Our present results clarified some differences existed in the localization of there drugs in sensitization models. Such variations may play important roles in the stimulant-derived psychosis.
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