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Analysis of human D2 dopamine receptor gene in schizophrenia

Research Project

Project/Area Number 02454291
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionNiigata University

Principal Investigator

KUWANO Ryozo (1991)  Niigata University, Research Laboratory for Molecular Genetics, Associate Professor, 遺伝子実験施設, 助教授 (20111734)

薄井 宏 (1990)  新潟大学, 脳研究所, 助手 (20192510)

Co-Investigator(Kenkyū-buntansha) MORII Ken  Niigata University, Research Laboratory for Molecular Genetics, Assistant, 遺伝子実験施設, 助手 (20230089)
ABE Satoshi  Niigata University, Brain Research Institute, Assistant, 脳研究所, 助手 (90202663)
USUI Hiroshi  Niigata University, Brain Research Institute, Assistant, 脳研究所, 助手 (20192510)
KUMANISHI Toshiro  Niigata University, Brain Research Institute, Professor, 脳研究所, 教授 (40018601)
桑野 良三  新潟大学, 遺伝子実験施設, 助教授 (20111734)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1991: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1990: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsD2 dopamine receptor / Gene Schizophrenia / Polymorphism / SSCP / SSCP法 / ド-パミンD_2受容体
Research Abstract

Dopamine and dopamine receptors may be involved in the movement and emotion balances. D2 dopamine receptor has high affinity for the antipsychotic drugs. Tliis receptor may also be related to the pathoctlenesis of Parkinson's disease and schizophrenia. We purified the bovine brain D2 dopamine receptor protein by photoaffinity labeling, high performance liquid chrom-atoaraphy and preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis. Further we analyzed electrophysiologically the D2 dopamine receptor expressed in Xenopus Oocyte after injection of poly(A)RNA. We attempted to analyze the structure of the D2 dopamine receptor gene in schizophrenia. We succeeded in cloning the genomic DNA for D2 dopamine receptor and clarified the structure of this gene. This gene spans 15 kbp and consists of 7 exons interrupted by 6'introns. Spot-blot hybridization analysis of fractionated human chromosomal DNA with D2 receptor "enomic DNA revealed the localization of this gene in the chromosome I 1. We analyzed human genomic DNA by Southem blot analysis, but so far we could not find restriction fragment length polymorphism(RFLP)in schizophrenia. We are trying to detect the differences of the sequences of dopamine D2 receptor gene between normal and schizophrenia by single strand conformation polymorphism(SSCP).

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] H.Usui: "Purification of D2 dopmamine receptor by photoaffinity labeling,high-performance liquid chromatography and preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis" J.Chromatography,. 515. 375-384 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Sakai.: "Dopamine receptors expressed in the Xenopus oocytes injecteted with bovine striatal mRNA." Mol.Brain Res.7. 183-187 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Araki: "Structure and expression of human and rat D2 dopamine receptor gene" Neurochem.Int.,. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Toshiaki Isobe: "Distunct forms of the protein kinase-dependent activcator of tyrosine and tryptophan hydroxylases" J.Mol.Biol.217. 125-132 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 荒木 一明: "人D2ド-パミン受容体の遺伝子解析" 精神薬療基金研究年報. 第21集. 233-237 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 荒木 一明: "ド-パミン受容体の遺伝子解析的研究" 精神薬療基金研究年報. 第22集. 194-197 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Usui, Y. Takahashi, N. Maeda, H. Mitui, T. Isobe, T. Okuyama, Y. Nishizawa and S. Hayashi: "Purification of D2 dopamine receptor by photoaffinity labeling, high-performance liquid chromatography and preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis" J. Chromatography. 515. 375-385 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Sakai, M. Sekiguchi, K. Okamoto, R. Kuwano and Y. Takahashi: "Dopamine receptors expressed in the Xenopus Oocytes injected with bovine striatal mRNA" Mol. Brain Res.7. 183-187 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Isobe, T. Ichimura, T. Sunaya, T. Okuyama, N. Takahashi, R. Kuwano and Y. Takahashi: "Distinct forms of the protein kinase-dependent activator of tyrosine and tryptophan hydroxylases" J. Mol. Biol.217. 125-132 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Araki, R. Kuwano, K. Morii, S. Hayashi S. Minoshima, N. Shimizu T. Katagiri, T. Kumanishi and Y. Takahashi: "Structure and expression of human and rat D2 dopamine receptor genes" Neurochem. Int.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Usui: "Purification of D2 dopmamine receptor by photoaffinity labeling highーperformance liquid chromatography and preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis" J.Chromatography. 515. 375-384 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Sakai.: "Dopamine receptors expressed in the Xenopus oocytes injecteted with bovine striatal mRNA." Mol.Brain Res.7. 183-187 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Araki: "Structure and expression of human and rat D2 dopamine receptor gene" Neurochem.Int.,. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshiaki Isobe: "Distunct forms of the protein kinaseーdependent activcator of tyrosine and tryptophan hydroxylases" J.Mol.Biol.217. 125-132 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 荒木 一明: "人D2ド-パミン受容体の遺伝子解析" 精神薬療基金研究年報. 第21集. 233-237 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 荒木 一明: "ド-パミン受容体の遺伝子解析的研究" 精神薬療基金研究年報. 第22集. 194-197 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Usui,Hiroshi: "Purification of D_2 dopamine receptor by photoaffinity labelling,highーperformance liquid chromatography and preparative sodium dodecyl salfate polyacrylamide gel electrophoresis." Jounal of Chromatography. 515. 375-384 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sakai,Yutaka: "Dopamine receptors expressed in the xenopus oocytes injected with bovine striatal mRNA" Molecular Brain Research. 7. 183-187 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kobayashi,Hisashi: "Stability of messenger RNA in postmortem human brain and construction of human brain cDNA libraries." Journal of Molecular Neuroscience. 2. 29-34 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 桑野 良三: "神経特異蛋白質の遺伝子発現調節" 代謝;臨時増刊「先端の医生物学とバイオサイエンス」. 257-261 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 桑野 良三: "Sー100β遺伝子" 医学のあゆみ. 115. 703-705 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Abe,Satoshi: ""Neoplastic angioendotheliosis":demonstration of immunoglobulin gene rearrangements by the Southern blot hybridization technique" Virchows Arch B. 58. 241-244 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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