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Long time preservation of whole & split liver with intermittent perfusion

Research Project

Project/Area Number 02454295
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionAkita University, Akita, Japan

Principal Investigator

KOYAMA Kenji  Akita University School of Medicine Professor, 医学部, 教授 (80004638)

Co-Investigator(Kenkyū-buntansha) OMOKAWA Susumu  ditto Lecturer, 医学部, 講師 (30221170)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1991: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1990: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsLiver Preservation / DNA Damage & Repair / Parenchimal & Nonparenchimal Cell of the Liver / Liver Mitochondria / Intermittent Liver Perfusion / DNA損傷・修復
Research Abstract

The liver for transplantation is preserved in cold solution, however, the viability or transplantability decreases with the lapse of time. Prolongation of the time limit for preservation is strongly requested for the convenience of the transplantation.
First, the viability tests of the liver preserved were studied using rats, and DNA damage and mitochondrial function, especially, the preservation of ATP synthesis after heat shock loading were strongly recommended.
Next, for prolongation of time limit, intermission perfusion for 1 hr with normothermic buffer solution was adopted to the rat liver during preservation in cold UW solution. Damage of cell membrane, protein synthesis and mitochondrial function were investigated, and following, results were obtained. Mitochondrial function was significantly improved by the perfusion. However, membrane damage of both parechymal and nonparenchymal cells measured by in situ trypanblue uptake was not improved by the perfusion. Furthermore, protein synthesis was not increased by perfusion.
These results indicate that the mitochondrial improvement was not enough to increase the protein synthesis of hepatocytes. However, when oxygen carrier of artificial blood was used as perfusate instead of UW solution mitochondrial function was highly reserved after presevation. for longer time. Therefore, some devices in both perfusate and perfusion conditions will provide the prolongation of the time limit of preservation.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 佐藤 勤: "肝実質細胞・非実質細胞の核.DNA損傷修復からみた肝保存法の評価." 移植. 26. 349-357 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 鹿嶋 秋五: "肝分離低温潅流法の確立" 秋田医学. 17. 729-736 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Koyama: "Ratioal procedure of hepatic vascular exclusion with hypothemic liver perfusion for complicated hepatic surgery" Hepato-Gastroenterology. 39. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsutomu Sato: "Effect of normothermic intermission perfusion on the liver during presevation in cold UW solution" Transplantation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shugo Kashima: "Establishment of isolated hypothermic perfusion of the liver. (Japanese with English summary)" Akita J. Med. 17. 729-736 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsutom Sato: "Evaluation of the liver preservation from the viewpoint of nuclear DNA damage and repair of hepatic parenchimal and nonparenchimal cells. (Japanese with English summary)" Jap. J. Transplantation. 26. 349-357 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Koyama: "Rational procedure of hepatic vascular exclusion with hypothermic liver perfusion for complicated hepatic surgery." Hepato-Gastroenterology. 39. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsutom Sato: "Effect of normothermic intermission perfusion on the liver during preservation in cold UW solution."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenji Koyama: "Rational procedure of hepatic vascular exclusion with hypothermic liver perfusion for complicated hepatic surgery" HepatoーGastroenterology. 39. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tsutom Sato: "Effect of normothermic intermission perfusion on the liver during preservation in cold UW solution" Transplantation.

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 勤: "肝実質細胞,非実質細胞の核DNA損傷・修復からみた肝保存法の評価" 移植. 20. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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