Venous and lymphatic anastomosis using a diode laser system
| Project/Area Number |
02454300
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
KONNO Hiroyuki Hamamatsu Univ, Sch. of Med., Assistant Professor, 医学部, 講師 (00138033)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Katsuhiro 浜松ホトニクス(株), 中央研究所, 室長代理
蜂谷 貴 浜松医科大学, 医学部, 助手 (70238020)
佐藤 勝彦 浜松ホトニクス株式会社中央研究所, 第7研究室, 室長代理
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | diode laser / venous anastomosis / lymphatic anastomosis / 半導体レ-ザ- |
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| Research Abstract |
The usefulness of a new diode laser system(Hamamatsu Photonics Co. Ltd., continuous mode, wavelength; 830nm, ceramic contact probe) for microvascular anastomosis of vein and lymph vessels was studied.
The femoral vein of wister rat weighing 250g was transected under microscope and four 10-0 polypropylene stay sutures were placed for edge coaptation. Laser contact probe was touched the anastomotic line to fuse the vein edges 2 - 5 seconds with the power of 60 mW. The laser anastomosis was made in forty rats and the conventional anastomosis with interrupted sutures using 10-0 polypropylene was made in forty rats for control group. The patency was observed for sixteen weeks and the histological examination was also performed in both groups. The anastomosis of lymphatics was made using the lymph vessel in the inguinal region of mongrel dogs(30 - 60 mW, 2 -3 seconds).
Patency rates were 95.0% in laser group and 92.5% in control group. Histological examination at two weeks revealed that in laser group intimal cell coverage was completed and the laminar structure of the media was well oriented, on the other hand, in control group, fibrous hyperplasia of the intima and cellular reaction around the suture material were observed. Laser anastomosis of the lymph vessels has technical difficulty to fuse the edges of lymph wall due to penetration or shrinkage of the vessel wall. Furthermore, the histological examination is not enough to observe the change of anastomotic site because of the difficulty to identify the line of anastomosis.
Laser amastomosis presents the more physiological wound healing and the characteristic is especially suitable for anastomosis of the veins which are easily thrombosed. Concerning the lymph anastomosis, no satisfactory result was obtained and a new device would be required.
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Report
(4 results)
Research Products
(5 results)
