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Prevention of canine acute hepatic rejection by ex vivo perfusion using anti-class II antibody.

Research Project

Project/Area Number 02454306
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionOkayama University Medical School

Principal Investigator

SAKAGAMI Kenichi  Okayama University Medical School Department of I Surgery Associate Professor, 医学部, 助教授 (40124789)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsLiver transplantation / acute rejection / class II antigen / anti-class II antibody / OKIal / artificial immunomodulation / OKIa1 / 体外潅流法 / 肝クッパ-細胞
Research Abstract

The aim of this study is to investigate whether anti-class II antibody (OKIa1) can block the class II antigens of hepatic sinusoidal lining cells (SLC) using ex vivo perfusion, and whether it can prevent acute rejection in dogs not given immunosuppressants. Canine liver transplantation was performed orthotopically, either with any treatment (group 1, n=4) or with ex vivo perfusion with anti-class II antibody (group 2, n=3). SLC were separated by the collagenase and pronase digestion methods, and then mixed lymphocyte-SLC cultures (MLSLC) were performed in each group. Hepatic grafts were examined histologically and the class II expression of sinusoidal endothelial cells (SEC) and kupffer cells (KC) were compared on day 7 postoperatively. The pretransplant MLSLC in group 2 showed significant inhibition of 60 <minus-plus> 7% compared with group 1. Liver enzyme and bilirubin were significantly high in group 1 than in group 2 on day 7. In the immunohistological studies for class II expression, SLC were stained more storngly in group 1 than in group 2. The flow cytometric analysis showed that class II expression on SEC and KC in group 1 on day 7 was 71% and 63%, respectively, and while for group 2, it was 27% and 30%, respectively. Histologically, livers in group 1 showed marked evidence of acute rejection, while the livers in group 2 had no evidence of acute rejection on day
CONCLUSION : These results indicate that ex vivo perfusion of the liver with anti-class II antibody could suppress the MLSLC and class II antigen expression on SLC after transplantation, and could significantly reduce acute rejection in canine liver transplantation.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Kurozumi Y, Sakagami K, et al.: "The effect of ex vivo perfusion of the liver with anticlass II antibody on acute rejection in canine liver transplantation." Transplantation. 52. 170-172 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kurozumi Y, Sakagami K, et al.: "Ex vivo perfusion of the liver with anti-class II antibody can prevent acute rejection in canine liver transplantation." Transplantation Proceedings. 23. 593-596 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takaishi Y, Sakagami K, et al.: "Ex vivo perfusion of the cluster graft with anti-class II antibody can significantly prevent acute rejection in swine abdominal organ cluster transplantation." Transplantation Proceedings. 25. 493-499 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyoshi K, Sakagami K, et al.: "Ex vivo perfusion of canine pancreaticoduodenal allografts using class II - specific monoclonal antibody delays the onset of acute rejection." Transplant International. 3. 151-155 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakagami K, Kurozumi Y, et al.: "Immunobiology of hepatic nonparenchymal cells and ex vivo perfusion of the livers with anti-class II antibody: a strategy to prevent hepatic rejection in the dog." Transplantation Proceedings. 27. 533-536 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kurozumi Y, Sakagami K et al.: "The effect of ex vivo perfusion of the liver with anti-class II antibody on acte rejection in canine liver transplantation." Transplantation. 52. 170-172 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kurozumi Y, Sakagami K et al.: "Ex vivo perfusion of the liver with anti-class II antibody can prevent acute rejection in canine liver transplantation." Transplantation Proceedings. 23. 593-596 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takaishi Y, Sakagami K et al.: "Ex vivo perfusion of the cluster graft with anticlass II antibody can significantly prevent acute rejection in swine abdominal organ cluster transplantation." Transplantation Proceedings. 25. 493-499 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Miyoshi K, Sakagami K et al.: "Ex vivo perfusion of canine pancreaticoduodenal allografts using class II-specific monoclonal antibody delays the onset of acute rejection." Transplant International. 3. 151-155 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakagami K, Kurozumi Y et al.: "Immunobiology of hepatic nonparenchymal cells and ex vivo perfusion of the livers with anti-class II antibody : A strategy to prevent hepatic rejection in the dog." Transplantation Proceedings. 27. 533-536 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 阪上 賢一: "ブタ肝/膵 十二指腸・脾同時分割移植術(スプリットクラスタ-手術)" 今日の移植. 4. 27-33 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 阪上 賢一: "ブタを用いた肝/膵・十二指腸・脾の同時分割移植術(スプリットクラスタ-手術)とその早期成績" 外科治療. 65. 527-529 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakagami K.: "A new splitting technique for simultaneous transplantation of liver/pancreas,duodenum and spleen in pigs." Transplantation Proceedings.

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakagami K.: "The impact of triple drug immunosuppression on clinical results of cadaveric kidney transplantation:a cumporrison of carrentional immunosuppresser." Acta Med Okayama.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kurozumi Y.: "The effect of ex vivo perfusion of the liver with antiーclassII,antibody on acute rejection in canine liver transplantation." Transplantation. 52. 170-172 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kurozumi Y.: "Ex vivo perfusion of the liver with antiーclassII antibody can peevent acute rejection in canine liver transplantation." Transplantation Proceedings.23. 593-596 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakagami,K.,Higaki,K.,Toda,K.,et al.: "Role of canine hepatic sinusoidal lining cells in the immune response。" Transplantation Proceedings. 21. 411-415 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sakagami,K.,Takasu,S.,Kawamura,T.,et al.: "A comparison of University of Wisconsin and EuroーCollins' solution for simple cold storage in nonーheartーbeating cadaveric kidney transplantation." Transplantation. 49. 824-826 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kurozumi,Y.,Sakagami,K.,Orita,K.: "Ex vivo perfusion of the liver with antiーclass II antibody can prevent acute rejection in canine liver transplantation." Transplantation Proceedings. 23. 593-596 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Inagaki,M.,Sakagami,K.,Oiwa,T.,et al.: "Systemic and local immune responses in allograft tolerance following swine liver transplantation." Transplantation Proceedings. 23. 561-564 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kobayashi,N.,Sakagami,K.,Takasu,S.,et al.: "Significance of pancreatic allograft rejection in swine abdominal organ cluster transplantation." Transplantation Proceedings. 23. 635-639 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hasuoka,H.,Sakagami,K.,Orita,K.: "A new slow delivery type of superoxide dismutase prevents warm ischemia damage in swine orthotopic liver transplantation." Transplantation Proceedings. 23. 693-696 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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