Experimental study for in utero repair of congenital diaphragmatic hernia
| Project/Area Number |
02454323
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Akita University |
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Principal Investigator |
KATO Tetsuo Akita University, Department of Surgery, Associate Professor, 医学部, 助教授 (20004963)
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| Co-Investigator(Kenkyū-buntansha) |
HEBIGUCHI Tatsuzo Akita University, Department of Surgery, Assistant Professor, 医学部, 講師 (00124644)
HIGUCHI Motokazu Akita University, Department of Gynecology, Associate Professor, 医学部, 助教授 (20006779)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | congenital diaphragmatic hernia / intra-uterine repair / hypoplastic lung / surfactant in the lung / DNA in the lung / histology of the lung / lung faction |
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| Research Abstract |
To study the efficacy and feasibility of in utero repair of congenital diaphragmatic hernia, we produced and repaired diaphragmatic hernias in fetal rabbits.
Diaphragmatic hernias were produced in 90 fetal rabbits at 22 days gestation (term=31 days) via a left thoracotomy. The diaphragmatic hernias were repaired at 25 days gestation in 50 rabbits, 20 of which subsequently died. The rabbits with repaired or unrepaired diaphragmatic hernia were dilivered at 29 days gestation by caesarean section.
The sacrificed rabbits were subjected to measurements of P-V curve and lung compliance, measurements of body and lung weight, determination of DNA and surfactant (DSPC and PG) content in the lungs, and histological examination of the lung.
The total lung weight/body weight ratio and total lung DNA content in the repaired group were greater than those in the unrepaired group. There was no difference between the 2 groups in body weight and surfactant content. Histologically, the lung acini and terminal air spaces were more mature in the repaired group than in the unrepaired group. Lung compliance in the repaired group was greater than that in the unrepaired group.
In conclusion, in utero repair of congenital diaphragmatic hernia is morphologically and functionally effective in improving the hypoplasticity of the lung which accompanies this lesion.
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Report
(4 results)
Research Products
(18 results)
