| Project/Area Number |
02454334
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | NATIONAL CARDIOVASCULAR CENTER RESEARCH INSTITUTE |
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Principal Investigator |
NAKATANI Takeshi National cardiovascular Center Research Institute Dept. of Biomedical Engineering, Division Head, 生体工学部, 室長 (60155752)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Tsuyoshi National Tosei Hospital Director General, 院長
KOSAKAI Yoshio National Cardiovascular Center Hospital Dept. of Cardiovascular Surgery, Senior, 病院, 医長
TAENAKA Yoshiyuki National Cardiovascular Center Research Institute Dept. of Artificial Organs, Di, 人工臓器部, 室長 (00142183)
MATSUDA Takehisa National Cardiovascular Center Research Institute Dept. of Biomedical Engineerin, 生体工学部, 部長 (60142189)
TAKANO Hisateru National Cardiovascular Center Research Institute Dept. of Artificial Organs, Di, 人工臓器部, 部長 (60028595)
岸田 晶夫 国立循環器病センター研究所, 生体工学部, 室長 (60224929)
巽 英介 国立循環器病センター研究所, 人工臓器部, 室員 (00216996)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Skeletal muscle / Collagen gel / Electrical conditioning / Cardiomyoplasty / Tissue regeneration / Hybrid artificial endocardium / Biomechanical pump / コラ-ゲンゲル |
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| Research Abstract |
We designed a totally implantable circulatory assist device consisting of a hybrid artificial ventricle composed of a skeletal muscle ventricle lined with a hybrid artificial endocardium. This hybrid artificial endocardium consist of a structural matrix made of a polyurethane porous membrane, fragment blood vessel, and collagen gel.
To clarify the ability of skeletal muscle as energy source, the effect of cardiomyoplasty on ventricular function was evaluated. The experiment was performed with the left latissimus dorsi muscle (LDM) in 4 normal heart goats. After electrically conditioned for 6 to 8 weeks, the pedicled LDM were sutured around the ventricle clockwise and stimulated synchronously with our compact electrical stimulator. To evaluated the ventricular function,systolic pulmonary arterial pressure (sPAP), right ventricular ejection fraction (RVEF), cardiac output (CO), systolic left ventricular pressure ; and maximal end-systolic volume-elactance (Ees) with and without stimulatio
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n were measured. Although no significant changes were observed in these data, sPAP RVEF, and CO showed a tendency to increase.
We prepared the polyurethane porous membrane by solvent cocasting with salt powder. We used collagen gel with fragmented goat carotid vein to perform in vitro construction of the hybrid artificial endocardium. For in vivo construction of the hybrid artificial endocardium, we used a modified version of the tissue fragment method for vascular prostheses. We prepared suspensions of tissue fragments using collagen gel with fragmented goat carotid vein. We used a highly porous fabric vascular prosthesis as a structural matrix; tissue fragments were entrapped on the outer surface of the prosthesis, and the prostheses then was implanted into the carotid artery of four adult goats.In specimens 1 and 3 months postimplantation, cells from the fragmented tissue regenerated an endothelium-like monolayer sheet on the inner suface of the prosthese. Output of a prototype bioaritifical ventricle reached 920 ml/min at an afterload of 80 mmHg and a preload of 20 mmHg.
Based on these data, the authors concluded the bioartificial ventricle cound play an important part to improve the right ventricular function and is promising as an implantable device with excellent antithrombogenicity. Less
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