| Project/Area Number |
02454336
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka University |
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Principal Investigator |
ARITA Norio Osaka Univ., Dept. of Neurosurg, 医学部, 助手 (80159508)
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| Co-Investigator(Kenkyū-buntansha) |
平賀 章寿 大阪大学, 医学部, 助手
OHNISHI Takanori Osaka Univ., Dept. of Neurosurg, 医学部, 助手 (70233210)
HAYAKAWA Toru Osaka Univ., Dept. of Neurosurg, 医学部, 教授 (20135700)
HIRAGA Syoju Osaka Univ., Dept. of Neurosurg
平賀 章壽 大阪大学, 医学部, 助手
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | brain tumor / glioma / chemotherapy / drug resistance / carcinogensis / p53 / extracellular matrix / tenascin / carcinogensis / invasion / oncogene / glioblastoma / tumor invasion / tumor suppressor gene |
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| Research Abstract |
ENU-induced in vitro carcinogenesis of rat fetal astroglia
To investigate glial carcinogenesis in vitro, fetal rat brain cells were cultured and exposed to ENU ( - 200mu g/ml). Cells were passaged weekly thereafter. When mutant colonies appeared. the cells were cloned. Both primary cultured and ENU-treated transformed cells were positive for GFAP and vimentin, but population of A2B5 positive cells was less than 5 %, thus indicating that these cells were astroglial in origin. The mutant colonies appeared 7 weeks after ENU treatment. The cloned mutant glial cell line formed large colonies in low(2%) serum medium and showed tumorigenicity in nude mice. By immunocytochemistry. p53 protein was never detected in non-transformed glial cells, but was positive in ENU-transformed cells. Norhern blot analysis showed over-expression of p53 mRNA in transformed cells. These results indicate that p53 mutation is related to transformation of rat fetal astroglias by ENU in vitro.
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