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The molecular biological research for disorder of neuron and synaptic formation of the brain in congenital hydrocephalus

Research Project

Project/Area Number 02454338
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionJuntendo University School of Medicine

Principal Investigator

SATO Kiyosi  Juntendo University School of Medicine Department of Neurosurgery Professor & Chirman, 医学部, 教授 (10112707)

Co-Investigator(Kenkyū-buntansha) MIYAJIMA Masakazu  Juntendo University School of Medicine Department of Neurosurgery Assistant, 医学部, 助手 (60200177)
SUDA Kikuo  Juntendo University School of Medicine Department of Neurosurgery Assistant, 医学部, 助手 (00206559)
TAKEDA Nobuaki  Juntendo University School of Medicine Department of Neurosurgery Assistant, 医学部, 助手 (00171645)
ARAI Hajime  Juntendo University School of Medicine Department of Neurosurgery Assistant Prof, 医学部, 講師 (70167229)
門田 静明  順天堂大学, 医学部, 助手 (60204517)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsCongenital Hydrocephalus / HTX-rats / Synapse Related Protein / Cytoskeletal Protein / Nerve Growth Factor / コリンアセチル基転換酵素 / 酵素免疫測定法 / Immunoblotting / 免疫組織化学染色 / tubulin / Tau蛋白 / MAP2 / 脳室腹腔短絡術 / SVPー38 / Drebrin
Research Abstract

In our experiments using congenitally hydrocephalic HTX-rats, we report some following matters concerned with disorder of neuron and synaptic formation of the brain.
1. Monoclonal antibodies against synaptic vesicle protein (SVP-38) and developmentally regulated brain proteins (Drebrins) were used to assess these two synapse related proteins by means of either a quantitative immunohistochemical method or a qualitative immunoblot analysis. The amount of SVP-38 decayed at 4 weeks after birth in hydrocephalic HTX-rats. When the hydrocephalic HTX-rats were treated with V-P shunt at 7 days after birth, such perturbations in postnatal changes of the SVP-38 were prevented completely. On the other hand postnatal decay of the embryonic form of Drebrins was considerably delayed in hydrocephalic HTX-rats. These observations suggest that synaptogenesis in the brain in the hydrocephalic HTX-rats is disturbed by the progression of hydrocephalus, and that early treatment of hydrocephalus may play a be … More neficial role not only in the repairing but also in the prevention of impaired synaptogenesis.
2. We investigated expressional and distributional changes of cytoskeletal proteins (tubulin, MAP-2 and tau protein) during developmental of brain in congenitally hydrocephalic HTX-rats using immunoblot analysis and immunohistochemical method. In the amount of and the distribution of tubulin and tau protein, there were no significant difference between non-hydrocephalic and hydrocephalic HTX-rats until 4 weeks of age. However, the amount of MAP-2, which is located in the dendrites of neurons, of 1 day old hydrocephalic rats have already decreased as compared with that of non-hydrocephalic rats at the same age. These observations seem to suggest that the metabolic disturbance of MAP-2 at the early developmental brain prevent the growth of dendrites of neurons and the maintenance of their final formation in the hydrocephalic brain.
3. Hydrocephalic associated changes in Choline Acetyltransferase (ChAT) activities and Nerve growth factor (NGF) in different areas (front-basal cortex, parieto-occipital cortex and hippocampus) of HTX-rat brains were determined. ChAT activity in the parieto-occipital cortex of 3 weeks old hydrocephalic rats was remarkable decreased, suggesting disturbance of the axonal transport of acetylcholine from the neurons in the basal forebrain to the parieto-occipital cortex. NGF concentration in the parieto-occipital cortex markedly increased with progression of hydrocephalus in comparison with that in the front-basal cortex and hippocampus. Such changes in regional increase of NGF may represent perturbation of neural mechanism in the developing brain affected by progressive hydrocephalus. Considering the experimental observations we have presented here, it would appear that one of the tasks required of pediatric neurosurgeons is the development of new surgical and medical treatment of congenital hydrocephalus in utero. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Miyazawa T et.al: "Impairment of synap to genesis and learning disability tn HTX-rats with arrested shunt-dependent hydrocephalus." Child's Nerve Syst. 7. 121-128 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Miyazawa T et.al: "Cortical Synaptogenesis in Congenitally Hydrocephalic HTX-Rats Using Monoclonal Anti-Synaptic Vesicle Protein Antiboby." Brain Dev. 14. 75-79 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 須田 喜久夫その他: "先天性水頭症における脳のSVP-38,Drebrin並びにNCAMの動態について" 小児の脳神経. 17. 101-106 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Suda et.al: "Hydrocephalus-Pathogenesis and Treatment" Springer-Varlag,Tokyo, 702 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 武田 信昭その他: "水頭症ー基礎と臨床ー" にゅーろん社,東京, 274 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Miyazawa T et.al: "Impairment of synaptogenesis and learning disability in HTX-rats with arrested shunt-dependent hydrocephalus." Child's Nerve Syst. 7. 121-128 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Miyazawa T et.al: "Cortical Synaptogenesis in Congenitally Hydrocephalic HTX-Rats Using Monoclonal Anti-Synaptic Vesicle Protein Antiboby." Brain Dev. 14. 75-79 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sudak et al: "Developmental Changes of Brain Proteins (SVP-38,Drebrins and NCAM) in HTX-rats with Congeital Hydrocephalus" Nerv Syst in Child. 17. 101-106 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Suda K et.al: Springer-Verlag,Tokyo. Hydrocephalus-Pathogenesis and Treatment, 10-26 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nibuaki T et al: Neuron-sha, Tokyo. Hydrocephalus, 109-124 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Miyazawa T et.al: "Impairment of synaptogenesis and learning disability in HTX-rats with arrested shunt-dependent hydrocephalus." Child's Nerve Syst. 7. 121-128 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miyazawa T et.al: "Cortical Synaptogenesis in Congenitally Hydrocephalic HTX-Rats Using Monoclonal Anti-Synaptic Vesicle Protein Antiboby." Brain Dev. 14. 75-79 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 須田 喜久夫 その他: "先天性水頭症における脳のSVP-38,Drebrin並びにNCAMの動態について" 小児の脳神経. 17. 101-106 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Suda K et.al: "Hydrocephalus-Pathogenesis and Treatment" Springer-Verlag,Tokyo, 702 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 武田 信昭 その他: "水頭症ー基礎と臨床ー" にゅーろん社、東京, 274 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Miyazawa T,Sato K et al.: "Learning disability and impairment of synaptogenesis in HTXーrats with arrested shuntーdependent hydrocephalus" Child's Nerv Syst. 7. 121-128 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Suda K,Sato K et al.: "Plasticity of Brain Development in Congenitally Hydrocephalic HTXーrats with Arrested Shuntー Dependent Hydrocephalus"

    • Related Report
      1991 Annual Research Report
  • [Publications] Suda K,Sato K et al.: "Developmental Change in SynapseーRelated Proteins(SVPー38 and Drebrin)in Congenitally Hydrocephalic HTXーrat with and without Early Placement of Ventriculoperitoneal Shunt"

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 潔 他: "水頭輪一基礎と臨床 先天性実験的水頭症モデルの病態" 松本 悟(永井書店),

    • Related Report
      1991 Annual Research Report
  • [Publications] 宮澤 隆仁他: "先天性水頭症ラットーHTXーにおける大脳皮質神経細胞の発達障害についてーGolgi studyー" 小児の脳神経. 13. 253-270 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮岡 誠他: "先天性水頭症ラットに対するVーPシャント前・後における脳局所グルコ-ス代謝の変化" 小児の脳神経. 13. 389-393 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Wada M.: "Congenital hydrocephalus in HTXーrats:incidence,pathophisiology,and developmental impairment." Neurologia medicoーchirurgica. 28. 955-964 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyaoka M.et al: "Measurement of local cerebral glucose utilization before and after VーP shunt in congenital hydrocephalus in rats." Metabloic Brain Disease. 3. 125-132 (1988)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyazawa T.et al: "A new method of quantitatively analyzing synapses using a synaptic vesicle protein (SVPー38) monoclonal antibody." in preparation.

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyazawa T.et al: "Impairment of synaptogenesis and learning desability in the HTXーrats with arrested shuntーdependent hydroephalus." Child Nervus System.

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤 潔他: "水頭症ー基礎と臨床ー先天性水頭症モデルの病態出版予定" 松本悟(永井書店),

    • Related Report
      1990 Annual Research Report
  • [Publications] Suda K.et al: "International Symposium on Hydrocephalus Early vs.Delayed Ventriculoperitoneal Shunt Effects on the Impairment of the Developing Brain in Congenitally Hydrocephalic HTXーrat." Springer Verlag.,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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