| Project/Area Number |
02454369
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | School of Medicine, Kanazawa University |
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Principal Investigator |
OHKAWA Mitsuo Department of Urology, School of Medicine, Kanazawa University, Associated Professor, 医学部, 助教授 (80019596)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Takao Department of Urology, Kanazawa University Hospital, Research Associate, 医学部附属病院, 助手 (20217706)
TOKUNAGA Shuji Department of Urology, Kanazawa University Hospital, Research Associate, 医学部附属病院, 助手 (70207554)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1991: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Candida albicans / Strain variation / Serotype / Mycelial form / Urinary candidiasis / IC_<30> / _D-arabinitol / Mannan / Candida glahurata / 上行性腎盂腎炎 / Wistar系ラットと水腎症 / 上行性Candida腎盂腎炎 / 30%inhibitory concentration(IC_<30>) / mitochondria DNA pattern / 血清中Dーarabinitol |
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| Research Abstract |
Although candiduria is not an infrequent finding, its clinical significance is uncertain. Therefore, we studied urinary candidiasis experimentally and clinically. Among Candida species, Candida albicans was the most frequently isolated from clinical specimens, followed by Candida tropicalis and Candida glabrata. Serotyping of the C. albicans isolates demonstrated that serotype A strains were more frequently isolated than serotype B ; the isolates of C. albicans serotype B showed a significantly lessened susceptibility to 5-fluorocytosine than those of serotype A, by means of a photo-read broth microdilution method for determination of 30% inhibitory concentration (IC_<30>) . We found that mitochondrial DNA restriction profiles of C. albicans strains isolated from urine specimens can be divided into several types. Ascending pyelonephritis in rats with incomplete ureteral obstruction, developed at a high rate, using both C. albicans serotype A strain and B strain ; the incidences were si
… More
milar. On the other hand, the incidence of ascending pyelonephritis using a C. glabrata strain and a mutant strain of C. albicans. which are not dimorphic and occur only as yeast cells, decreased significantly compared with those using C. albicans strains. In addition, germinated C. albicans cells were observed to adhere to the urothelium of the rat bladder more easily than yeast-form organisms. These results strongly suggest that the mycelial form is important for tissue invasion to the urinary tract. A clinical study of candiduria patients revealed that disturbance of urine flow, antimicrobial chemotherapy and indwelling urinary catheters are important factors predisposing to candiduria. We demonstrated that there are statistically significant relationships between renal. and urinary Candida colony counts, and between renal Candida populations and grades of renal histological changes, using two new experimental models of ascending. C. albicans pyelonephritis in rats. Based on our experimental and clinical data, serum _D-arabinitol and mannan are considered to be useful markers in distinguishing invasive urinary candidiasis form benign colonization. The data on the serum _D-arabinitol/creatinine ratios in urinary candidiasis patients suggest that 2 weeks or more may be an appropriate duration of treatment for urinary candidiasis. Less
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