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Molecular biological study on genesis and proliferation of renal malignant tumor

Research Project

Project/Area Number 02454373
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionKeio University

Principal Investigator

HAYASHI Satoru (1992)  Keio Univ., Sch. of Med. Dept. of Urology, Assistant, 医学部, 助手 (60180965)

畠 亮 (1990-1991)  慶應義塾大学, 医学部, 助教授 (40051586)

Co-Investigator(Kenkyū-buntansha) HATA Makoto  Tokyo Dental College. Dept. of Urology, Professor, 歯学部, 教授 (40051586)
SAITO Shiro  Keio Univ., Sch. of Med. Dept. of Urology, Assistant, 医学部, 助手 (80170504)
BABA Shiro  Keio Univ., Sch. of Med. Dept. of Urology, Assistant Professor, 医学部, 講師 (00051889)
林 暁  慶應義塾大学, 医学部, 助手 (60180965)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1992: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsRenal Cell Carcinoma / Chromosom / G-banding technique / Transitional Cell Carcinoma / H-ras oncogene / Point Mutation / Hーras / オンコジン / 泌尿器系腫瘍
Research Abstract

We started this study in aiming to clarify the mechanism of genesis and proliferation of renal malignant tumors by means of molecular biology technique utilizing tissue materials surgically extirpated from the patients with renal cell carcinoma.
In the 1st year, chromosomal analysis was carried out on 8 tumors of renal cell carcinoma. There were relatively high incidence of numerical aberrations in #3 and #7 chromosoms, and a lesser incidence of constitutional aberrations, i.e. long-arm aberrations in #2 and #6 chromosoms.
In the 2nd year, DNAs were extracted from these 8 tumors and then subjected to evaluation of various oncogene expression. However, no significant abnormaility was found. On the other hand, it was successful to detect over expression of H-ras oncogene and its point mutation by means of polymerase chain reaction(PCR) method in 2 out of 50 bladder cancers (4%). In this method, the DNA segments including codon 12 were amplified by PCR, and the reaction products were examined for their susceptibility to the restriction enzyme, and by dot blot hybridization assay with oligonucleotide probes.
Point mutations were detectable in small amounts of DNAs isolated from fresh or frozen tumor tissues, urine cells and paraffin sections. These results indicated that our method was not superior as screening,but very useful in following up the readily positive cases. Particularly emphasized is feasibility of urine cells as test samples because of their non invasiveness to the patients and easy repeatability.
In the 3rd year, we made efforts to try to publicate our results.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 畠 亮: "腎細胞癌の染色体分析" 日本泌尿器科学会雑誌. 81. 1389-1395 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 畠 亮: "単腎あるいは両側性発生腎腺癌に対する腎保存手術ーとくに腫瘍検出術の適応と限界についてー" 日本泌尿器科学会雑誌. 82. 412-419 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TACHIBANA M.: "Bromodeoxy wuidine and deoxyribonucheic acid fitariate analysis in human renal cel carcinoma." American J.of Clinical Pathology. 97. 538-47 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 中島 淳: "腎細胞癌患者における内因性サイトカイン産生に関する研究" 日本泌尿器科学会雑誌. 83. 1110-1117 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAITO Shiro: "Detection of H-ras Gene Point mutetions in Transitional cell Carcinoma of Human Urirary Bladder Using Polynerase Chain Reaction" Keio J.Medicine. 41. 80-86 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAITO Shiro: "Detection of H-ras Gene Point mutation in 50 Cases of Bladder Carcinoma." Cancer Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hata,M., Baba,S., Saito,S., Tachibana,M., Deguchi,N., Jitsukawa,S. and Tazaki,H.: "Chromosomal analysis of renal cell carcinoma" Jpn. J. of Urology. 81(9). 1389-1395 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hata,M., Baba,S., Tachibana,M., Deguchi,N., Jitsukawa,S. and Tazaki,H.: "Kidney preserving surgery for renal cell carcinoma in patients with a solitary kidney or bilateral renal tumors" Jpn. J. of Urology. 82(3). 412-419 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Tachibana,M., Deguchi,N., Baba,S., Jitsukawa,S. and Tazaki,H.: "Bromodeoxyuridine and deoxyribonucleic acid bivariate analysis in human renal cell carcinoma" American J. of Clinical Pathology. 97(5). 538-547 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakashima,J., Tachibana,M., Baba,S., Deguchi,N., Jitsukawa,S., Hata,M., and Tazaki,H.: "Studies on the production of endogenous cytokines in patients with renal cell carcinoma" Jpn. J. of Urology. 83(7). 1110-1117 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito,S.: "Detection of H-ras gene point mutations in transitional cell carcinoma of human urinary bladder using polymerase chain reaction" Keio J. Medicine. 41(2). 80-86 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito,S., Makoto,H., Fukuyama,R., Sakai,K., Kudoh,J., Tazaki,H. and Shimizu,N.: "Detection of H-ras gene point mutations in 50 cases of bladder carcinoma" Cancer Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] TACHIBANA M.: "Bromodeoxy.uridine and deoxyribonucleic acid bivariate analysis in human renal cell carcinoma" American J.of clinical Pathology. 97. 538-47 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 中島 淳: "腎細胞癌患者における内因性サイトカイン産生に関する研究" 日本泌尿器科学会雑誌. 83. 1110-1117 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] SAITO Shiro: "Detection of H-ras Geme Point mutation in transitional cell ceranoma of human urinary Bladder using Polymerase chain Reaction." Keio J.Medicino. 41. 80-86 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] SAITO Shiro: "Detection of H-ras Gene Point mutation in 50 Cases of Bladder Carcinoma." Cancer Research.

    • Related Report
      1992 Annual Research Report
  • [Publications] HATA,M.: "Nephron conserving surgery for renal cell corcinoma of early stage by tumor enucleation" J.Jpn.SoC.Concer Ther.26. 132 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 畠 亮: "単腎あるいは両側性発生腎腺癌に対する腎保存手術ーとくに腫瘍核出術の適応と限界について" 日本泌尿器科学会雑誌. 82. 412-419 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 宍戸 清一郎: "偶発腎細胞癌の臨床病理学的検討およびflow cytometryによる細胞生物学的持性の解析" 日材泌尿器科学会雑誌. 82. 87-93 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] SAITOH,S.: "Detection of Hーras gone point mutations in 50 cases of bladder carcinoma." Cancer Research.

    • Related Report
      1991 Annual Research Report
  • [Publications] SAITOH,S.: "Detection of Hーras gone point mutations in transitional cell carcinoma of human urinary bladder using polymerase chain reaction" Keio J.Medicine.

    • Related Report
      1991 Annual Research Report
  • [Publications] 畠 亮: "腎細胞癌の染色体分析" 日本泌尿器科学会雑誌. 81. 1389-1395 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 畠 亮: "単腎あるいは両側性発性腎腺癌に対する腎保存手術" 日本泌尿器科学会雑誌.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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