| Project/Area Number |
02454377
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Tokyo |
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Principal Investigator |
TAKETANI Yuji University of Tokyo, Faculty of Medicine, Department of Obstetrics and Gynecology.Professor., 医学部(病), 教授 (10114539)
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| Co-Investigator(Kenkyū-buntansha) |
SHAN-GUANG Liang University of Tokyo, Faculty of Medicine, Department of Obstetrics and Gynecolog, 医学部(病), 助手 (20220808)
TUTUMI Osamu University of Tokyo, Faculty of Medicine, Department of Obstetrics and Gynecolog, 医学部(病), 講師 (60134574)
百枝 幹雄 東京大学, 医学部(病)・産婦人科, 助手 (50221627)
林 直樹 東京大学, 医学部(病)・産婦人科, 助手 (60211489)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1990: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | implantation / epidermal growth factor / endometrium / prostaglandin / tissue-plasminogen activator / EGF receptor / folliculogenesis / embryogenesis / activin / follistatin / epidermal growth factor / follicle / granulosa cells / FSH / CAMP / progesterone / estrogen / EGF受容体 / 胚発生 / prostaglandin E_2 / tissueーplasminogen activator / early embryos / embryonel development |
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| Research Abstract |
The present study was undertaken to investigate the mechanism of implantation taking the embryo-maternal interaction into account. First of all, epidermal growth factor (EGF) -like immunoreactivity was demonstrated in human endometrium along with the receptor for EGF.In primary cell culture of human endometrial cells, EGF stimulated the release of both prostaglandin and tissue-plasminogen activator which are supposed to be involved in implantation. EGF was further shown to enhance glycogenolysis so as to supply embryos with glucose requires for nidation. Altogether, EGF is implicated to have a role in implantation. Another finding with EGF is that estradiol increased the amount of EGF receptor in combination with progesterone in human endometrial cells. As for folliculogenesis, EGF stimulated the proliferation of cultured rat granulosa cells in concert with FSH.In view of the presence of EGF-like substance in the ovaries, EGF is assumed to regulate folliculogenesis. In addition, EGF stimulated the development of mouse 2-cell embryo in vitro. Thus, it appears that EGF has diverse biological actions in reproduction, such as folliculogenesis, embryonal development and implantation, etc.
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