| Project/Area Number |
02454414
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo Medical & Dental University |
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Principal Investigator |
ETO Kazuhiro Tokyo Med & Dent Univ, Fac Dent, Prof., 歯学部, 教授 (30014161)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Transgenic mouse / Facial malformation / Transthyretin gene / Facial development / Ossification / Abnormal occlusion / 異型トランスサイレチン遺伝子 |
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| Research Abstract |
A line of transgenic mice has previously been produced by inducing the human mutant transthyretin gene in an effort to construct a mouse model of familial amyloid polyneuropathy. This mouse shows an autosomal mutation of facial development. The facial malformation was characterized by a short snout and a twisted upper jaw. In order to analyze the primary event of the abnormal facial development, embryos of this mutant mouse were observed morphologically on 11.5, 13.5, 15.5 and 17.5 days of gestation(plug day = 0).
In the 11.5-day embryos, when the facial primordia were formed, facial abnormalities were not observed. In embryos of 13.5-day and afterwards, facial abnormalities were observed at dissection and light microscopic levels. Abnormal embryos were a little small compared to normal ones and showed -the short snout and also the deep fissure in the center of the upper lip. Light microscopic observations revealed that some of the facial bones and muscles were hypoplastic in embryos of 15.5 and 17.5-day. From these results, the facial abnormalities in embryos firstly detected on 13.5-day were proved to be similar to those in an adult transgenic mice. It is suggested that the short snout and abnormal ossification of facial bones are related to abnormal behavior of cranial neural crest cells. Abnormal occlusion caused by the twisted upper jaw is assumed to be secondarily induced on the weak bone and muscle tissues by mastication after weaning.
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