• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of an alveolar bone resorption inhibitor that suppresses interleukin-1

Research Project

Project/Area Number 02454479
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionNational Institute of Health

Principal Investigator

KOGA Toshihiko  NIH, Dept. of Oral Science, Director, 口腔科学部, 部長 (10037541)

Co-Investigator(Kenkyū-buntansha) AKADA Hiromasa  NIH, Dept. of Oral Science, Chief, 口腔科学部, 主任研究官 (40072920)
西原 達次  国立予防衛生研究所, 口腔科学部, 主任研究官 (80192251)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1992: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsIL-1 / IL-1 inhibitor / periodontitis / bone resorption / macrophage / インローロイキン1 / インターロイキン1インヒビター / リポ多糖 / ILー1 / ILー1インヒビタ- / マクロファ-ジ / マクロフィ-ジ
Research Abstract

Periodontitis is characterized by alveolar bone loss. Interleukin-1 (IL-1) produced by macrophages and polymorphonuclear leukocytes is considered to play a central role in the bone resorption at inflammatory regions in periodontitis. In this study, we purified an IL-1 inhibitor from culture supernatants of macrophages and examined whether it suppresses the bone resorption.
IL-1 inhibitor was purified from culture supernatants of P388D_1 cell line murine macrophages stimulated with lipopolysaccharide for 72 h to homogeneity by a five-step procedure: acetic acid extraction from culture supernatants; Bio-Gel P-60 gel chromatography; DEAE-Sepharose CL-6B column chromatography; reverse-phase high-performance liquid chromatography on a C_<18> hydrophobic support; and high-performance liquid chromatography on a gel filtration column. The purified IL-1 inhibitor gave a single band of protein with a molecular mass of 67 kDa on SDS-polyacrylamide gel electrophoresis, and had an isoelectric point of 4.8. The IL-1 inhibitor was a heat- and acid-stable protein that was inactivated by digestion with trypsin and reduction with dithiothreitol. The IL-1 inhibitor suppressed murine and human IL-1-induced proliferation of C3H/HeJ mouse thymocytes. However, the IL-1 inhibitor did not affect the proliferation of IL-2-dependent CTLL-2 cells induced by IL-2 and the proliferation of C3H/HeJ mouse thymocytes stimulated with a mitogenic dose of concanavalin A. The inhibitor at final concentrations of 1 to 5 mug/ml inhibited almost completely in vitro IL-1 induced bone resorption of BALB/c mouse calvaria, suggesting that the inhibitor may be useful for control of bone resorption in periodontitis.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Udagawa,N.他7名: "Origin of osteoclasts:nature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenviroment prepared by bone marrow derived stromal cells" Proc.Nat1.Acad.Sci.USA. 87. 7260-7264 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koga,T.他4名: "Monoclonal antibody-coated latex agglutination assay for identification of Actinobacillus actinomvcetemcomitans." Zbl.Bakt.274. 91-100 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takahashi,T.他6名: "Murine macrophage interleukin-1 release by capsularlike serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans" Infect.Immun.59. 18-23 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shibuya,N.他6名: "6-Deocy-D-talan and 6-deoxy-L-talan:novel serotypespecific polysaccharide antigens from Actinobacillus actiomycetemcomitans" J.Biol.Chem.266. 16318-16323 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ishihara,Y.他4名: "Role of interleukin-1 and prostaglandin in in virto bone resorption induced by Actinobacillus actinomycetemcomitans lipopolysaccharide" J.Periodont.Res.26. 155-160 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato,S.他6名: "Construction of serotype b-sprcific polysaccharide antigen-defective mutants of Actinobacillus actinomycetemcomitans by insertion of transposon Tn916" J.Gen.Microbiol.138. 1203-1209 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Udagawa,N., et al.: "Origin of osteoclasts; mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow derived stromal cells" Proc. Natl. Acad. Sci. USA. 87. 7260-7264 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Koga,T., et al.: "Monoclonal antibody-coated latex agglutination assay for identification of Actinobacillus actinomycetemcomitans." Zbl. Bakt.274. 91-100 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takahashi,T., et al.: "Murine macrophage interleukin-1 release by capsularlike serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans" Infect. Immun.59. 18-23 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shibuya,N., et al.: "6-Deoxy-D-talan and 6-deoxy-L-talan: novel serotype-specific polysaccharide antigens from Actinobacillus actinomycetemcomitans" J. Biol. Chem.266. 16318-16323 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ishihara,Y., et al.: "Role of interleukin-1 and prostaglandin in in vitro bone resorption induced by Actinobacillus actinomycetemcomitans lipopolysaccharide" J. Periodont. Res.26. 155-160 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato,S., et al.: "Construction of serotype b-specific polysaccharide antigen-defective mutants of Actinobacillus actinomycetemcomitans by insertion of transposon Tn916" J. Gen. Microbiol.138. 1203-1209 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato,S.他6名: "Construction of serotype b-specific polysaccharide antingen-defective mutants of Actinobacillus actinomycetemcomitans by insertion of transposon Tn916" J.Gen.Microbiol.138. 1203-1209 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Koga,T.他5名: "The 64-kilodalton GroEL-like protein of Actinobacillus actinomycetemcomitans" J.Periodont.Res.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高橋 一郎 他1名: "Epitope scanningーマルチピンテクノロジー利用した抗原エピトープの解析" 生体防御. 9. 107-112 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡橋 暢夫 他1名: "合成ペプチドを用いたエピトープの同定法" 実験医学. 10. 85-89 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Ishihara et al.: "Role of interleukin-1 and prostaglandin in in vitro bone resorption induced by Actinobacillus actinomycetemcomitans lipopoly saccharide" J.Periodont.Res.26. 155-160 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Shibuya et al.: "6-Deoxy-D-talan and 6-deoxy-L-talan:novel serotype-specific polysaccharide antigens from Actinobacillus actinomycetemcomitans" J.Biol.Chem.266. 16318-16323 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Sato et al.: "Construction of serotype 6-specific polysaccharide antigen-defective mutants of Actinofacillus actinomycetemcomitans by insertion of transposon Tn 916" J.Gen.Microbiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] 古賀 敏比古ら: "歯周治療の科学" 青野正男(医歯薬出版), 267 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Koga et al: "Periodontal disease:pathogens & host immune responses" S.Hamada,S.C.Holt and J.R.Mc Ghee(Quentessence Publishing Co.), 405 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Udagawa et al.: "Origin of osteoclasts:mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrowーderived stromal cells" Proc.Natl.Acad.Sci.USA. 87. 7260-7264 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Takada et al.: "<Bacteroides>___ー lipopolysaccharides (LPS) induce anaphylactoid and lethal reactions in LPSーresponsive andーnonresponsive mice primed with murmyldipeptide" J.Infect.Dis.162. 428-434 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Koga et al.: "Monoclonal antibodyーcoated latex agglutination assay for identification of <Actinobacillus>___ー <actinomycetemcomitans>___ー" Zbl.Bakt.274. 91-100 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Hamada et al.: "Antimicrobial activities thiolactomycin against gramーnegative anaerobes associated with periodontal disease" Oral Microbiol.Immunol.5. 340-345 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Takahashi et al: "Murine macrophage interleukinー1 release by capsularlike serotypeーspecific polysaccharide antigens of <Actinobacillus>___ー <actinomycetemcomitans>___ー" Infect.Immun.59. 18-23 (1991)

    • Related Report
      1990 Annual Research Report

URL: 

Published: 1990-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi