| Project/Area Number |
02454480
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
MASUJIMA Tsutomu Inst. Pharmaceutical Sci. Hiroshima Univ., Professor, 医学部, 助教授 (10136054)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Ikue Inst. Pharmaceutical Sci. Hiroshima Univ., Prsearch Associate, 医学部, 助手 (60174421)
SUGAWARA Yoshiaki Hiroshima Women's University, Home Economics, Associate Prof., 家政学部, 助教授 (30154462)
SUGIYAMA Masanori Inst. Pharmaceutical Sci. Hiroshima Univ., Associate Prof., 医学部, 助教授 (30106801)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1990: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | X-ray photoacoustics / Laser photoacoustics / Biological microanalysis / Photoacoustic microscopy / Photoacoustic imaging / Laser photoacoustics / Biological microanalvsis / Xーray Photoacoustics / Laser Photoacoustics / Biological Microanalysis / Photoacoustic Microscopy / Photoacoustic Imaging |
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| Research Abstract |
A new microscopy which scans focused X-ray and laser bean, on the sample was developed to get photoacoustic sional simultaneously. Synchrotron radiation was utilized as X-ray source. In order to aet hidh spatial resolution, sensitivity of X-ray photoacoustic detection and noise level of measurement system were improved. The subtraction image at the X-ray enermies above and below Cu, Ni k-edoe was obtained to get hiqhliohted redon of each species. Laser pohotoacoustic microscopy was applied to the imacina analysis of dispersion of the bence-jones protein in human fetal pancreas tissue-sections.
Combining the two microscopic systems into one system. which has a special cell with two windows (one for X-ray and another for laser beam), new microsconv-was developed.
On the course of this study, X-ray luminescence was found by using monitor video camera. Startine from this founding, video microsconic imaoe analysis was also developed and andlied to the analysis of living cells.
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