| Project/Area Number |
02454526
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURE COLONY |
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Principal Investigator |
WATANABE Tomomasa INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURE COLONY, DEPARTMENT OF BIOCHEMISTRY, HEAD OF GROUP, 生化学部, 室長 (10100174)
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| Co-Investigator(Kenkyū-buntansha) |
MASAKI Shigeo INSTITUTE FOR DEVELOPMENTAL RESEARCH, AICHI PREFECTURE COLONY, INSTITUTE FOR DEV, 生化学部, 主任研究員 (10157175)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1992: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | mouse / DNA polymorphism / RFLP / chromosome mapping / backcross / MRL strain / autoimmune disease / linkage / PCR多型 / 野生マウス / 形態形成異常 / マウス第5染色体 / MRLマウス / 自己免疫疾患遺伝子 / リンケ-ジ / スフィンゴミエリネ-ス / ニ-マンピック病 |
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| Research Abstract |
The lpr (lymphoproliferation) gene, which is an autosomal recessive gene causing autoimmune disease with features of human systemic lupus erythematosus and iventually death from severe immune-complex glomerulonephritis, has been mapped on mouse chromosome 19. To determine the exact chromosomal location of the lpr gene, the interspecific backcross was carried out by the mating (MRL/MpJ-lpr/lpr x MOL-TIT)x MRL/MpJ-lpr/lpr. Ly-44 (lymphocyte differentiation antigen-44) and Tdt (terminal deoxynucleotidyl transerase) were used as molecular marker genes on chromosome 19. RFLP of Ly-44 (lymphocyte differentiation antigen-44) and Tdt were in the case of BamHI. The following order of genes is proposed with the distances between genes given in parenthese: centromere - Ly-44 - (19.3cM) - lpr - (6.1cM) - Tdt - telomere. Therefore, the lpr gene is mapped at position 23 on mouse chromosome 19.
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