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Genetic Ecology of Bradyrhizobium japonicum in soils

Research Project

Project/Area Number 02455007
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionIBARAKI UNIVERSITY

Principal Investigator

ASAMI Teruo  IBARAKI UNIVERSITY FACULTY OF AGRICULTURE TITLE OF POSITION: PROFESSOR, 農学部, 教授 (20011877)

Co-Investigator(Kenkyū-buntansha) MINAMISAWA Kiwamu  IBARAKI UNIVERSITY FACULTY OF AGRICULTURE TITLE OF POSITION: ASSOCIATE PROFESSOR, 農学部, 助教授 (70167667)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsBradyrhizobium / root nodule / soybean / host specificity / fingerprint / signal / genetic ecology / 根粒菌 / DNA / フィンガ-プリント / 反復配列 / 多様性
Research Abstract

The isolates of Bradyrhizobium japonicum indigenous to six field in Japan were characterized by using four DNA hybridization probes from the species; nifDK, nodDYAB, hup, RS-alpha, and RS- beta. Diversity in RS-specific hybridization (RS-fingerprint) were observed. The RS-fingerprints were correlated with the nif and hup genotypes, and with the location of field including cropping history. According to nodDYAB hybridization, the field isolates were classified into three main group A, B and C. The group A and B produced similar nod metabolites including NodBj-V with methyl fucose. The group C isolates, which were separated only from siratro, were incompatible with soybeans,and produced different nod metabolites. As for nodulation, we constructed simple relationship. As a result, we established the method of genetic ecology for indigenous bradyrhizobia.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kiwamu Minanisawa: "Genetic Relatedness of Bradyrhizobium japonicum Field Isolates as Revealed by Repeated Saguencos and Various Other Characteristics" Applied and Environmental Microbiology. 58. 2832-2839 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 南沢 究: "インドール酢酸生産能によるダイズ根粒菌の系統分類法" 日本土壌肥料学雑誌. 63. 459-462 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 南沢 究: "ダイズ根粒菌の系統と生態" 土の微生物. 40. 37-46 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Minamisawa, T. Seki, S. Onodera, M. Kubota, T. Asami: "Genetic relatedness of Bradyrhizobium japonicum field isolates as revealed by repeated sequences and various other characteristics" Appl. Environ. Microbiol.58. 2832-2839 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Minamisawa, H. Iino, N. Saito, S. Onodera, Y. Nakatsuka, S. Nakazima, M. Kubota, T. Asami: "Determination of genotype group of Bradyrhizobium japonicum by indole-3-acetic acid production." Jpn. J. Soil Sci. Plant Nutr.63. 459-462 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K. Minamisawa: "Phylogeny and ecology of Bradyrhizobium japonicum" Soil Microorganisms. 40. 37-46 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kiwamu Minamisawa: "Genetic Relatedness of Bradyrhizolbium japonicum Field Isolates as Revealed by Repeated Seguences and Various Other Characteristics" Applied and Environmental Microbiology. 58. 2832-2839 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 南沢 究: "インドール酢酸生産能によるダイズ根粒菌の系統分類法" 日本土壌肥料学雑誌. 63. 459-462 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 南沢 究: "ダイズ根粒菌の系統と生態" 土と微生物. 40. 37-46 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Kiwamu Minamisawa: "Gonetic relatedness of Brodyrhizobium japonicun field isolates as reveald by repeated sequences and various characteristics" Applied and Environmental Mecrobiology.

    • Related Report
      1991 Annual Research Report
  • [Publications] 南沢 究: "インド-ル酢酸生産能によるダイズ根粒菌の系統分類法" 日本土壌肥料学雑誌(第4号). 63. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kiwamu Minamisawa: "Genetic structure of a indigenous Bradyrhizobium japonicum population as revealed by repeated sequences and various characteristics" Applied and Environmental Microbiology.

    • Related Report
      1990 Annual Research Report
  • [Publications] 南沢 究: "土壌環境ー保全と機能の増進ー(IGEシリ-ズ13)土壌中におけるダイズ根粒菌の遺伝的多様性と生態(分担執筆,p33ー38)" 東北大学遺伝生態研究センタ-(発行者), 70 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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