| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Research Abstract |
The isolates of Bradyrhizobium japonicum indigenous to six field in Japan were characterized by using four DNA hybridization probes from the species; nifDK, nodDYAB, hup, RS-alpha, and RS- beta. Diversity in RS-specific hybridization (RS-fingerprint) were observed. The RS-fingerprints were correlated with the nif and hup genotypes, and with the location of field including cropping history. According to nodDYAB hybridization, the field isolates were classified into three main group A, B and C. The group A and B produced similar nod metabolites including NodBj-V with methyl fucose. The group C isolates, which were separated only from siratro, were incompatible with soybeans,and produced different nod metabolites. As for nodulation, we constructed simple relationship. As a result, we established the method of genetic ecology for indigenous bradyrhizobia.
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