| Project/Area Number |
02556010
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MIYAZAWA Teruo Tohoku Univ. Fac. Agric., Associate Professor, 農学部, 助教授 (20157639)
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| Co-Investigator(Kenkyū-buntansha) |
KAWABATA Sohei Tohoku Electr. Ind. Co., Head Researcher, 開発部, 研究室長
OIKAWA Shinichi Tohoku Univ. Fac. Med., Assistant Professor, 医学部, 助手 (30142946)
ENDO Yasushi Tohoku Univ. Fac. Agric., Assistant Professor, 農学部, 助手 (60194049)
FUJIMOTO Kenshiro Tohoku Univ. Fac. Agric., Professor, 農学部, 教授 (00005620)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | lipid peroxidation / peroxidized lipid / lipid hydroperoxide / chemiluminescence / luminol / cytochrome c / 高速液体クロマトグラフィ- / ルミノ-ル / 過酸化リン脂質 / ヒドロペルオキシド |
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| Research Abstract |
A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was developed and applied for the hydroperoxide-specific determination of phosphatidylcholine hydroperoxide (PCOOH), phosphatidylethanolamine hydroperoxide (PEOOH), triacylglycerol hydroperoxide (TGOOH), squalene hydroperoxide (SQOOH)and 8a-hydroperoxy tocopherone (TocOOH) in biological tissues such as human blood plasma, plasma lipoproteins, erythrocytes, cultured diploid cells, and liver, brain, heart, lung of the rodents.
The method involves separation of lipid hydroperoxide in each lipid class from total lipids of the biological tissues with HPLC column and post-column detection of hydroperoxide-dependent chemiluminescence of lipid hydroperoxides. The chemiluminescence is produced by luminol oxidation during a reaction of hydroperoxide and cytochrome c-heme. The high specificity for hydroperoxide group enables a sensitive assay for a wide range of hydroperoxides, with the detection limit of 1 picomole of hydroperoxide-O_2. By use of this method, the presence of phospholipid hydroperoxides in human blood plasma and erythrocytes is confirmed quantitatively.
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