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Developmental study on trichloroethylene degradation by a bioreactor

Research Project

Project/Area Number 02556014
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・発酵学
Research InstitutionNational Institute for Environmental Sciences

Principal Investigator

UCHIYAMA Hiroo  Nat'l Ins For Environ St, Water & Soil Environ Div, Senior researcher, 水土壌圏環境部, 主任研究員 (00185042)

Co-Investigator(Kenkyū-buntansha) IWASAKI Kazuhiro  ibid., Regional & Commun Environ Div, Researcher, 地域環境研究グループ, 研究員 (30193717)
YAGI Osami  Nat'l Inst For Environ St, Water & Soil Environ Div, Chief, 水土壌圏環境部, 室長 (40132865)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordstrichloroethylene / bioreactor / biodegradation / immobilized-cell / バイオリアクタ- / 固定化 / アルギン酸ゲル / 生分解 / 固定化菌体
Research Abstract

1. Characterization of liquid-solid phase trichloroethylene (TCE) degradation by Methylocystis sp. M (strain M) immobilized in Ca alginate was studied to maximize the degradation rate. The results obtained were as follows. (1) In order to get the cells of strain M possessing high specific activity for TCE degradation, elimination of Cu (almost to zero) in the medium and harvesting the cells at the end of the log phase were very important. (2 ) Kinetics constants (V max and Ks) and optimum conditions for TCE degradation (pH, temperature, DO) by immobilized cells were cleared. (3) The half life of the degradability of strain M was relatively short, and which was caused by the toxicity of intermediates formed by TCE degradation in the cells. (4) Inactivated cells by the intracellular toxicity were reactivated by incubation with methane or methanol, suggesting that the immobilized cells was possible to be repeatedly used in the TCE degradation. (5) Under the optimum conditions obtained, semicontinuous TCE degradation in a 5L bioreactor showed that the total amount of approximately 13mg TCE could be degraded in 120h.
2. We also studied on the gas-solid phase TCE degradation system by the immobilized cells. The gaseous TCE of 0.3ppm, which was fed continuous into the glass-column bioreactor, was almost completely degraded in about 50h. The maximum TCE concentration to be degraded by this reactor was 100ppm, suggesting that, as the gaseous TCE concentration exhausted from ducts of TCE treatment facilities was too high (300-500ppm), the gaseous TCE was necessary to be diluted and be applied to the bioreactor.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] H.UCHIYAMA他: "Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp.strain M" Applied and Environmental Microbiology. 58. 3067-3071 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] O.YAGI他: "Biodegradation rate of chloroethylene in soil environment" Water Science Technology. 25. 419-424 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.UCHIYAMA他: "Trichloroethylene degradation by immobilized resting-cells of Methylocystis sp.M in a gas-solid bioreactor" Biotechnology Letters. 14. 619-622 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 矢木 修身他: "揮発性有機塩素化合物の微生物による分解、除去" 水環境学会誌. 15. 493-498 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 内山 裕夫: "地下水汚染・土壌汚染の現況と浄化対策" 工業技術会, 340 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Uchiyama et al.: "Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp. strain M" Appl. Environ. Microbiol.58. 3067-3071 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] O. Yagi et al.: "Biodegradation rate of chloroethylene in soil environment" Water. Sci. Technol.25. 419-424 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H. Uchiyama et al.: "Trichloroethylene degradation by immobilized resting-cells of Methylocystis sp. M in a gas solid bioreactor" Biotech. Letters. 14. 619-622 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] O. Yagi et al.: "Microbial degradation of volatile chlorinated organic compounds" J. Japan Soc. Water Environ.15. 493-498 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Uchiyamaら: "Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp.strain M" Applied and Environmental Microbiology. 58. 3067-3071 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] O.Yagiら: "Biodegradation rate of chloroethylene in soil environment" Water Science Technology. 25. 419-424 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Uchiyamaら: "Trichloroethylene degradation by immobilized resting-cells of Methylocystis sp.M in a gas-solid bioreactor" Biotechnology Letters. 14. 619-622 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 矢木 修身ら: "揮発性有機塩素化合物の微生物による分解,除去" 水環境学会誌. 15. 493-498 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 内山 裕夫: "地下水汚染・土壌汚染の現況と浄化対策" 工業技術会, 340 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Nakajima et al.: "Novel metabolite of trichloroethylene in a methanotrophic bacterium,Methylocystis sp.M,and hypothetical degradation pathway" Biosci.Biotech.Biochem.56(3). (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Nakajima et al.: "Purification and properties of a soluble methane monooxygenase from Methylocystis sp.M" Biosci.Biotech.Biochem.56(5). (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Uchiyama et al.: "Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp.M" Appl.Environ.Microbiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] 矢木 修身,内山 裕夫: "水質汚濁対策へのバイオテクノロジ-の活用" 瀬戸内海科学. 2. 36-41 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 矢木 修身,内山 裕夫: "揮発性塩素化脂肪族炭化水素の微生物分解" 微生物. 5. 13-22 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 矢木 修身,内山 裕夫: "微生物によるクロロエチレン類の分解" 土と微生物. 37. 37-44 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 内山 裕夫,矢木 修身: "微量元素・化学物質と農業生態系ー有機ハロゲン化合物の生分解ー" 養賢堂, 284 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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