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DEVELOPMENT OF NEW SYSTEM USED CAGED-COMPOUND BY WHICH THE PHYSIOLOGICAL FUNCTION OF CNS NEURONS CAN BE CLARIFIED.

Research Project

Project/Area Number 02557005
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

AKAIKE Norio  TOHOKU UNIV.NEUROPHYSIOL.PROF., 医学部, 教授 (30040182)

Co-Investigator(Kenkyū-buntansha) YASUI Shouzo  KYUSHU INST.TECHNOL.CONTROL.ENGIN.SCI.PROF., 情報工学, 教授 (50132741)
KOIDE Ikuo  INTERDEC CHIEF, 課長
KUDO Yoshihisa  MITSUBISHI KASEI INST.LIFE SCI.DIRECT., 主任研究員
NABEKURA Junich  TOHOKU UNIV.NEUROPHYSIOL.ASSIST.PROF., 医学部, 助手 (50237583)
KAWA Kazuyoshi  TOHOKU UNIV.NEUROPHYSIOL.ASSOCI.PROF., 医学部, 助教授 (70125839)
徳冨 直史  東北大学, 医学部, 助手 (30227582)
中江 俊夫  東北大学, 医学部, 助手 (20155659)
喜納 兼勇  (株)同仁化学研究所, 部長
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 1992: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥11,000,000 (Direct Cost: ¥11,000,000)
KeywordsMultifunction simultaneous recording system / Fluorescent die / Electrophysiology / Rat / Acutely dissociated neurons / 多現象同時計測装置 / Caged化合物 / イオン測定蛍光試薬 / 単離中枢神経細胞 / 電気生理学 / caged化合物 / cagcd化合物
Research Abstract

In this study, the theme was focused on the following two points ; 1) development of the new recording system by which the patch-clamp and fluorometrical recordings can be performed simultaneously, 2) application of caged compound to CNS neurons acutely dissociated from mammalian brain. Improved trial system was used to investigate the details of the responses via M3 muscarinic acetylcholine, metabotropic glutamate and serotonin_3 receptors. As results, it was revealed that each receptor induces hyperpolarization via following pathway ; G-protein* PLC * IP_3 * Ca^<2+> release from intracellular Ca^<2+> store * K^+ channel open. On the other hand, to obtain an effective caged compound, we newly synthesized twelve caged glycines and searched the convenient one, because it was found from the preliminary experiment that the liberation efficiency of the commercially available compounds was quietly low. Quantitative analysis revealed N-[1-(2-Nitrophenyl) ethyloxycarbonyl] glycine as the most effectively photolyzed one. Glycine was liberalized UV energy dependently in as sigmoidal manner, and the liberation efficiency was 60 to 80 % between the concentration range from 10^<-5> to 10^<-3> M.
As described above, more than 60 % of initial plane was successfully finished, but many problems were also found in the fluorometrical measurement system and the combination of it with patch-clamp technique by the detailed biological experiment used trial system. A part of our results for fluorometrical measurement has been putted into the development of the succeeding system. Since the improvement of system was delayed so much, we could not put the system into practical use. However, the points to carry out the experiment was realized. The development of new membrane-permeable and low cell toxic fluorescent die is also necessary, because cell damage by loading of fura 2/AM caused the difficult patch-clamp experiment.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] AIBARA,K.,EBIHARA,S., & AKAIKE,N.: "Voltage-dependent ionic currents in dissodiated paratracheal ganglion cells of the rat." J.of Physiology. 457. 591-610 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] FURUKAWA,K.,AKAIKE,N.,ONODERA,H. & KOGURE,K.: "Expression of 5-HT_3 receptors in PC12 cells treated with NGF and 8-Br-cAMP." J.of Neurophysiology. 67. 812-819 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] ITABASHI,S.,AIBARA,K.,SASAKI,H. & AKAIKE,N.: "γ-Aminobutyric acid-induced response in rat dissociated paratracheal ganglion cells." J.of Neurophysiology. 67. 1367-1374 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] EBIHARA,S. & AKAIKE,N.: "Strychnine-induced potassium current in CA1 pyramidal neurones of the rat hippocampus." Br.J.Pharmacol.106. 823-827 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] AKAIKE,N.,HARATA,N.,UENO,S. & TATEISHI,N.: "GABAergic synaptic current in dissociated necleus basalis of meynert neurons of the rat." Brain Res.570. 102-108 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] EBIHARA,S.,TAKISHIMA,T.,SHIRASAKI,T. & AKAIKE,N.: "Regional variation of excitatory and inhibitory amino acid-induced responses in rat dissociated CNS neurons." Neuroscience Res.14. 61-71 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] HARATA,N.,EBIHARA,S., & AKAIKE,N.: "Modulation of NMDA receptors by ACh in the central nervous system." Prog.Neuro-Psychophamacol. & Biol.Psychiat.16. 977-984 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] AKAIKE,N.,YAMANAKA,H. & MUNAKATA,M.: "Novel Ca^<2+> currents in mammalian CNS neurons" Prog.Neuro-Psychophamacol. & Biol.Psychiat.16. 948-957 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] UENO,S.,KAKEHATA,S., & AKAIKE,N.: "Nicotinic acetylcholine receptor in dissociated rat nucleus tractus solitarii neurons." Neuroscience Lett.149. 15-18 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] UNEYAMA,H.,UENO,S. & AKAIKE,N.: "Serotonin-operated potassium current in CA1 neurons dissociated from rat hippocampus." J.of Neurophysiol.69. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] UNEYAMA,H.,MUNAKATA,M. & AKAIKE,N.: "Caffeine response in pyramidal neurons freshly dissociated from rat hippocampus." Brain Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] UNEYAMA,H.,HARATA,N. & AKAIKE,N.: "Caffeine and related compounds block inhibitory amino acid-gated Cl- currents in freshly dissociated rat hippocampal neurones" Br.J.of Pharmacol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] UENO,S.,NABEKURA,J.,ISHIBASHI,H.,AKAIKE,N.,MORI,T. & SHIGA,M.: "Photolysis of a newly synthesized caged glycine activates glycine receptor of rat CNS neurons." Neuroscience Method.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aibara, K., Ebihara, S. & Akaike, N.: "Voltage-dependent ionic current in dissociated paratracheal ganglion cells of the rat. J.Physiol." 457. 591-610 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Furukawa, K., Akaike, N., Onodera, H. & Kogure, K.: "Expression of 5-HT_3 receptors in PC12 cells treated with NGF and 8-Br-cAMP. J.Neurophysiol." 67. 812-819 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Itabashi, S., Aibara, K., Sasaki, H. & Akaike, N.: "gamma-Aminobutyric acid-induced response in rat dissociated paratracheal ganglion cells. J.Neurophysiol." 67. 1367-1374 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ebihara, S. & Akaike, N.: "Strychnine-induced potassium current in CA1 pyramidal neurones of the rat hippocampus. Br.J.Pharmacol." 106. 823-827 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Akaike, N., Harata, N., Ueno, S. & Tateishi, N.: "GABAergic synaptic current in dissociated nucleus basalis of Meynert neurons of the rat. Brain Res." 570. 102-108 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ebihara, S., Takishima, T., Shirasaki, T. & Akaike, N.: "Regional variation of excitatory and inhibitory amino acid-induced responses in rat dissociated CNS neurons. Neurosci. Res." 14. 61-71 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Akaike, N., Yamanaka, H. & Munakata, M.: "Novel Ca^<2+> currents in mammalian CNS neurons. Prog.Neuro-Psychopharmacol. & Biol. Psychiat." 16. 943-957 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Harata, N., Ebihara, S. & Akaike, N.: "Modulation of NMDA receptors by ACh in the central nervous system. Prog.Neuro-Psychopharmacol. & Biol. Psychiat." 16. 977-984 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Ueno, S., Kakehata, S. & Akaike, N.: "Nicotinic acetylcholine receptor in dissociated rat nucleus tractus solitarii neurons. Neurosci. Lett." 149. 15-18 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Uneyama, H., Ueno, S. & Akaike, N.: Serotonin-operated potassium current in CA1 neurons dissociated from rat hippocampus. J.Neuro-physiol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Uneyama, Munakata, M. & Akaike, N.: Caffeine response in pyramidal neurons freshly dissociated from rat hippocampus. Brain Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Uneyama, H., Harata, N. & Akaike, N.: Caffeine and related compounds block inhibitory amino acid-gated Cl^- currents in freshly dissociated rat hippocampal neurones. BR.J.Pharmacol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Aibara,K.,Ebihara,S.,& Akaike,N.: "Boltage-dependent ionic currents in dissodiated paretracheal ganglion cells of the rat." J.of Physiology. 457. 591-610 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Furukawa,K.,Akaike,N.,Onodera,H.& Kogure,K.: "Expression of 5-HT D23 D2 receptors in PC12 cells treated with NGF and 8-Br-cAMP" J.of Neurophysiology. 67. 812-819 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Itabashi,S.,Aibara,K.,Sasaki,H.& Akaike,N.: "γ-Aminobutyric acid-induced response in rat dissociated paratracheal ganglion cells." J.of Neurophysiology. 67. 1367-1374 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ebihara,S.& Akaike,N.: "Strychnine-induced potassium current in CA1 pyramidal neurones of the rat hippocampus." Br.J.Pharmacol.106. 823-827 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Akaike,N.,Harata,N.,Ueno,S.,& Tareishi,N.: "GABAergic symaptic current in dissociated necleus basalis of meynert neurons of the rat." Brain Res.570. 102-108 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ebihara,s.,Takishima,T.,Shirasaki,T.,& Akaike,N.: "Regional variation of excitatory and inhibitory amino acid-induced responses in rat dissociated CNS neurons." Neurosience Res.14. 61-71 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Harata,N.,Ebigara,S.,& Akaike,N.: "Modulation of NMDA receptors by ACh in the central nervous system." Prog.Neuro-psychophamacol.& Biol.Psychiat.16. 977-984 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Akaike,N.,Yamanaka,H.& Munakata,M.: "Novel Ca D12+ D1 currents in mammalian CNS neurons" Prog.Neuro-Psychopharamacol.& Biol.Psychiat.16. 948-957 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Ueno,S.,Kakahata,S.,& Akaike,N.: "Nicotinic acetylcholine receptor in dissociated rat nucleus tractus solitarii neurons." Neuroscience Lett.149. 15-18 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Uneyama,H.,Ueno,S.,& Akaike,N.: "Serotonin-operated potassium current in CA1 neurons dissociated from rat hippocampus." J.of Neurophysiol.69. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Uneyama,H.,Munakata,M.& Akaike,N.: "Caffeine response in pyramidal neurons freshly dissociated from rat hippocampus." Brain Res.

    • Related Report
      1992 Annual Research Report
  • [Publications] Uneyama,H.,Harata,N.,& Akaike,N.: "Caffeine and related compounds block inhibitory amino acid-gated cl-currents in freshly dissociated rat hippocampal neurones" Br.J.of Pharmacol.

    • Related Report
      1992 Annual Research Report
  • [Publications] Ueno,S.,Nabekura,J.,Ishibashi,H.,Akaike,N.,Mori,T.,& Shiga,M.: "Photolysis of a newly synthesized caged glycine activates glycine receptor of rat CNS neurons." Neuroscience Method.

    • Related Report
      1992 Annual Research Report
  • [Publications] Aibara,K.& AKaike,N.: "Acetylcholine-activated ionic currents in isolated paratracheal ganglion cells of the rat." Brain Res.558. 20-26 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shirasaki,T.,Klee,M.R.,Nakaye,T.& Akaike,N.: "Differential blockade of bicuculline and strychnine on GABA-and glycine-induced responses in dissociated rat hippocampal pyramidal cells." Brain Res.561. 77-83 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takahashi,K.,Ueno,S.& AKaike,N.: "Kinetic properties of T-type Ca^<2+> currents in isolated rat hippocampal CA1 pyramidal neurons." J.Neurophysiol.65. 148-155 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Harata,N.,Tateishi,N.& Akaike,N.: "Acetylcholine receptors in dissociated nucleus basalis of Meynert neurons of the rat." Neurosci.Lett.130. 153-156 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Akaike,N.,Harata,N.& Tateishi,N.: "Modulatory action of cholinergic drugs on N-methyl-D-aspartate response in dissociated rat nucleus basalis of Meynert neurons." Neurosci.Lett.130. 243-247 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Akaike,N.,Harata,N.,Ueno,S.& Tateishi,N.: "GABAergic synaptic current in dissociated nucleus basalis of Meynert neurons of the rat." Brain Res.570. 102-108 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takahashi,K.,Ueno,S.and Akaike,N.: "Kinetic properties of Tーtype^<2+> Currents in isolated rat hippocampal CA1 pyramidal neurons." Journal of Neurophysiology. 65(1). 148-155 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takahashi,K.and Akaike,N.: "Calcium antagonist effects on lowーthreshold (Tーtype) calcium current in rat isolated hippocampal CA1." The Journal of Pharmacology and Experimental Therapeutics. 256(1). 165-175 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ito,C.,Wakamori,M.and Akaike,N.: "Dual effect of glycine on isolated rat suprachiasmatic neurons." American Journal of Physiology. 260. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nakagawa,T.,Wakamori,M.,Shirasaki,T.,Nakaye,T. and Akaike,N.: "γーAminobutyric acidーinduced response in acutely isolated nucleus solitarii neurons of the rat." American Journal of Physiology. 260. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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