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Development of Analytical Instruments and Establishment of Analytical Method for Volatile Drugs and Poisons in Minute Amounts of Biological Samples

Research Project

Project/Area Number 02557032
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionDepartment of Legal Medicine,Kamazawa University School of Medicine

Principal Investigator

NAGANO Taizo  Dept. of Legal Med. Kanazawa Univ. School of Med., Professor, 医学部, 教授 (20073679)

Co-Investigator(Kenkyū-buntansha) MAEDA Hitoshi  Dept of Legal Med., Osaka City Univ. Med. School, Professor, 医学部, 教授 (20135049)
TATSUNO Yoshitsugu  Dept. of Legal Med. Kobe Univ. School of Med.,Professor, 医学部, 教授 (80030831)
KOJIMA Tohru  Dept. of Legal Med., Hiroshima Univ. School of Med.,Professor, 医学部, 教授 (70037399)
SHINOHARA Toshiaki  National lnst. of Police Science, Director of Identification Center, 附属鑑定所長
OGURI Naoki  Japan Analytical Industry, Vice-president, 研究所, 主任研究員
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 1991: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1990: ¥11,300,000 (Direct Cost: ¥11,300,000)
Keywordsminute amounts of biological sample / volatile drugs and poisons / Pulse heating / ferromagnetic alloy / measurement procedure / analytical instrument / emergency toxicology screening
Research Abstract

The present research has been performed in order to develop analytical instruments and to establish the procedures, being capable of accurate and rapid analysis for volatile substances (e.g., ethanol, chloroform and toluene) even in minute amounts of biological specimens. The results obtained are as follows;
1. In order to reveal the possibility of repeated usage of ferromagnetic alloys, special aluminum capsules were made experimentally to prevent contamination after analysis and deterioration of ferromagnetic alloys.
2. In fundamental experiments with animal tissue samples for ethanol, diethylether, chloroform and toluene, the measured values by the pulse heating method almost correlated to those by the head space method, respectively. It was also revealed that the pulse heating method could be available for the determination of all of the volatiles examined.
3. Sample specimens were divided into 5 portions, and the each specimen was examined simultaneously at 5 different institutes:
1) … More In ethanol concentrations measured values were compared to each other; between institute A and B (correlation coefficient(r)=0.943); between A and C (r=0.928); between A and D (r=0.990); between A and E (r=0.956), indicating strong positive correlations.
2)The concentrations of diethylether, chloroform and toluene were also compared. The correlation coefficient between A and C was 0.601 and between A and E, 0.858. These coefficients wide variations. The problem required a further investigation.
4. Ethanol concentrations in the blood and urine obtained from autopsied cadavers were measured by the pulse Heating and the head space methods. The measured values by the two different methods showed the following regression formula : Y=0.117+0.993X (Y, the measured value by the pulse method; X, those by the head space method; r=0.996; sample size, n=85), indicating a strong positive correlation
From these results, it has been revealed that pulse heating method was available for accurate and rapid analysis for ethanol in minute amounts of samples in both qualitative and quantitative analysis. The data obtained by measuring of diethylether, chloroform and toluene showed a wide variation among the different institutes. This required a further investigation. The pulse heating is, very useful for rapid qualitative screening of volatiles. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] T.Nagano,T.Takayasu T.Ohshima,H.Maeda: "An analytical method for volitiles in the biological materials by pulse heating" Proc.1st.Int.Symp.Advances in legal medicine. 323-325 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Nagano,H.Maeda,T.Takayasu,J.Nishigami,T.Ohshima: "Screening and quantitation of drugs and poisons in blood and urine in emergency medical care and legal autopsy caces" Proc.1st.Int.Symp.Advances In Legal Medicine. 368-370 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 永野 耐造,大島 徹,高安 達典,西上 潤,朱 宝利,前田 均: "救急医療をめぐる臨床法医中毒学的研究(第4報) Pulse heating GC法,GC-HS法及びCOオキシメーターによる薬毒物分析の実際" 日法医誌. 45. 197 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大島 徹,高安 達典,西上 潤,斉藤 和則,前田 均,永野 耐造: "救急医療をめぐる臨床法医中毒学的研究(第3報) 救急患者及び剖検試料からの犯法的薬毒物の分析" 日法医誌. 45. 39 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N.Oguri: "Rapid discrimination method for the animal fat using pyrolyzergas chromatograph" Proc.1st.Int.Symp.Advances In Legal Medicine. 528-530 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Adachi,Y.Tatsuno Y.Ogawa,K.Nakagawa,Y.Mizoi: "Greenish discoloation of skin and formation of sulfhemoglobin caused by hydrogen sulfide poisoning and putrefaction" Proc.1st.Int.Symp.Advances In Legal Medicine. 311-313 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Nagano, T. Takayasu, T.Ohshima & H. Maeda: "An analytical method for volatiles in the biological materials by pulse heating." Proc. 1st Int. Symp. Advances in Legal Med.323-325 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Nagano, H. Maeda, T. Takayasu, J. Nishigami & T. Ohshima: "Screening and quantitation of drugs and poisons in blood and urine in emergency medical care and legal autopsy cases." Proc. 1st Int. Symp. Advances in Legal Mid.368-370 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Nagano, T. Ohshima, T. Takayasu, J. Nishigami, B. Zhu & H. Maeda: "Forensic toxicological studies in emergency medical practice (IV) Analysis of drugs and poisons by pulse heating GC, GC-MS and CO oximeter." Jpn. J. Legal Med.45, suppl. 197 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Ohshima, T. Takayasu, J. Nishigami, K. Saito, H. Maeda & T. Nagano: "Forensic toxicological studies in emergency medical practice (III) Analysis of illicit drugs and poisons in blood and urine of emergency and autopsy cases." Jpn. J. Legal Mid.45, suppl. 39 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. Oguri: "Rapid discrimination method for the animal fat using pyrolyzer-gas chromatograph." Proc. 1st Int. Symp. Advances in Legal Med.528-530 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Adachi, Y. Tatsuno, Y. Ogawa, K. Nakagawa & Y. Mizoi: "Greenish discoloation of skin and formation of sulfhemoglobin caused by hydrogen sulfide poisoning and putrefaction." Proc. 1st Int. Symp. Advances in Legal Med.311-313 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Takayasu, T. Ohshima, T. Nakaya, J. Nishigami, T. Kondo, Z. Lin & T. Nagano: "Screening and quantitation of volatile substances by pulse heating method." Proc. 12th Int. Confer. Alcohol, Drugs, Traffic Safety.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Iwasaki, M. Miyazaki, M. Yashiki & T. Kojima: "Analysis of thinner components using the Curie-point pyrolyzer-gas chromatographic method in the tissues of a victim fatally poisoned." Jpn. J. Lega Med.46. 271-275 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Iwasaki, M. Miyazaki, M. Yashiki & T. Kojima: "Analysis of thinner components using of a victim fatally poisoned." Proc. 30th Int. Meeting of The Int. Assoc. Forensic Toxicologists.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Nagano, T. Ohshima, T. Takayasu, J. Nishigami, T. Nakaya & T. Sawaguchi: "A system of emergency toxicology screening for drugs and poisons." Proc. 30th Int. Meeting of The Int. Assoc. Forensic Toxicologists.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Nagano,T.Takayasu,T.Ohshima,H.Maeda: "An analytical method for volitiles in the biological materials by pulse heating" Proc.1st.Symp.Advances in legal medicine. 323-325 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Nagano,H.Maeda,T.Takayasu,J.Nishigami,T.Ohshima: "Screening and quantitation of drugs and poisons in blood and urine in emergency medical care and legal autopsy caces" Proc.1st.Int.Symp.Advances In Legal Medicine. 368-370 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 永野 耐造,大島 徹,高安 達典,西上 潤,朱 宝利,前田 均: "救急医療をめぐる臨床法医中毒学的研究(第4報)Pulse heating GC法,GC-MS法及びCOオキシメ-タ-による薬毒物分析の実際" 日法医誌. 45. -197 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大島 徹,高安 達典,西上 潤,斉藤 和則,前田 均,永野 耐造: "救急医療をめぐる臨床法医中毒学的研究(第3報)救急患者及び剖検試料かりの犯法的薬毒物の分析" 日法医誌. 45. -39 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Oguri: "Rapid discrimination method for the animal fat using pyrolyzergas chromatograph" Proc.1st.Int.Symp.Advances In Legal Medicine. 528-530 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Adachi,Y.Tatsuno,Y.Ogawa,K.Nakagawa,Y.Mizoi: "Greenish discoloation of skin and formation of sulfhemoglobin caused by hydrogen sulfide poisoning and putrefaction" Proc.1st.Int.Symp.Advances In Legal Medicine. 311-313 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Nagano,T.Takayasu,T.Ohshima,H.Maeda: "An alanytical method for volatiles in the biological materials by pulse heating" Proc.1st.Int.Symp.Advances Legal Mes.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 永野 耐造,大島 徹,高安 達典,西上 潤,朱 宝利,前田 均: "救急医療をめぐる臨床法医中毒学的研究(第4報)Pulse heating GC法、GCーMS法及びCOメ-タ-による薬毒物分析の実際" 日法医誌. 45. 197 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高安 達典,西上 潤,前田 均,永野 耐造: "薬毒物中毒に関する法医中毒学的スクリ-ニング2.Pulse heating(Py)ーGCとGCーMSによる分析" 日法医誌. 44. 55 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Miyazaki,T.Kojima,M.Yashiki,F.Chidasue,I.Tsukune: "Correlation between "on admession" blood toluene concentrations and the presence or absense of signs and symproms in solbent abusers" Forensic Sci.Int.44. 169-177 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Oguri: "Rapid discrimination method for the animal fat using pyrolyzerーgas chromatograph" Proc.1st.Int.Symp.Advances Legal Med.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Adachi,Y.Tatsuno,Y.Ogawa,K.Nakagawa,Y.Mizoi: "Greenish discoloation of skin and formation of sulfhemoglobin caused by hydrogen sulfide poisoning and putrefaction" Proc.1st.Int.Symp.Advances Legal Med.(1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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