| Project/Area Number |
02557038
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Tokyo |
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Principal Investigator |
YAZAKI Yoshio University of Tokyo, Faculty of Medicine, professor, 医学部(病), 教授 (20101090)
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| Co-Investigator(Kenkyū-buntansha) |
KATOH Hirohisa Yamasa Co.Ltd., Research and Development Division, Staff, 研究開発部・生物第2研究室, 研究員
SUGI Masato Yamasa Co.Ltd., Research and Development Division, Senior Staff, 研究開発部・生物第2研究室, 主任
YAMAZAKI Tsutomu University of Tokyo, Faculty of Medicine, Assistant, 医学部(病), 助手
SEKO Yoshinori University of Tokyo, Faculty of Medicine, Assistant, 医学部(病), 助手
YAMAOKI Kazuhide University of Tokyo, Health Administration Center for Students, Assistant Profes, 保健センター, 講師 (70182409)
栗原 裕基 東京大学, 医学部(病), 助手
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1992: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | RGD / Acute Myocardial Infarction / Myocardial Reperfusion Injury / Cell Adhesion Molecules / Antibody treatment / CD11a, b, c / 心筋虚血再潅流 / DD11a,b,c / CD54(ICAM-1) / CD62(GMP-140) / CD11a,b,c / CD54(ICAMー1) / CD18 |
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| Research Abstract |
The migration of neutrophils into tissues is the central event in myocardial hypoxiareoxygenation (H/R) as well as in inflammatory responses. The rolling of neutrophils has been revealed to be the first step of the interaction of the neutrophils with the vessel wall during an inflammatory response, which is dependent of the expression of granule membrane protein-140 (GMP-140) on the surface of vascular endothelial cells. First, using an in vitro model of H/R,we showed that higher number of neutrophils adhered to human umbilical vein endothelial cells (HUVECs) subjected to 60 minutes of hypoxia followed by 30 minutes of reoxygenation compared with neutrophils adhered to HUVECs subjected to no stimulation of I/R,and that preincubation of HUVECs with anti-human GMP-140 antibody blocked these adhesion. Furthermore, immunoperoxidase staining clarified, for the first time, that H/R enhanced the expression of GMP-140 on the surface of HUVECs. Next, using an in vivo rat model of myocardial ischemiareperfusion (I/R), we indicated that within 2 hours after reperfusion leukocytes began to infiltrate into the rat myocardia subjected to 30 minutes of ischemia, and clarified, for the first time, that the expression of intercellular adhesion molecule-1 (ICAM-1) was enhanced on the capillary and venous endothelial cells from 8 to 96 hours after the start of reperfusion. Furthermore, pretreatment with individual monoclonal antibodies against cell-adhesion molecules (CD11a, CD11b+c, CD18 and ICAM-1) reduced not only the infiltration of leukocytes but also the area of infarction in the reperfused hearts. Our present study suggests that the expression of GMP-140 along with ICAM-1 on vascular endothelial cells play a critical role in myocardial injury induced by I/R.
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