| Project/Area Number |
02557058
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
麻酔学
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
SHIMOJI Koki Niigata University School of Medicine Professor, 医学部, 教授 (30040158)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Naoshi Niigata University School of Medicine Lecturer, 医学部附属病院, 講師 (70181419)
IIDA Seishi Nagaoka Univ of Technology Dept of Electrical Engineering Professor, 工学部, 教授 (90126467)
SAKUDA Kyohei Nagaoka Univ of Technology Dept of Electrical Engineering Professor, 工学部, 教授 (60143814)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1992: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | Fine fiberscope / Fluorescence imaging / Fluorescence spectroscopy / Autofluorescence / Brain ischemia / NAD(P)H / 脳組織自家蛍光 / 自家蛍光画像 / 蛍光スペクトル / 石光ファイバー / 色素レーザー / レ-ザ- / 自家蛍光 / ファイバ-スコ-プ / NADH / 色素レ-ザ- |
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| Research Abstract |
Fine fiberscope systems for fluorescence observation of epidural and subarachnoid spaces were developed, and applications of the systems for monitoring of tissue oxygenation were investigated. A fiberscope system that was applied to fluorescence imaging is consisted of a Xe-lamp as a light source, a quartz fiber assembly (outer diameter:1.0mm)for irradiation of ultra-violet light, a fine endoscope, highly sensitive SIT camera and a video-imaging system. Although autofluorescence of the rat brain induced by irradiation of ultra-violet light (350nm) was week, it was clearly observed with this system. The brain tissue and the surface vessels were distinguishable in the endoscopic image of autofluorescence. Another fiberscope system is used for fluorescence spectrometry, and it is consisted of a laser-generator, a quartz fiber assembly (outer diameter:1.0mm) for irradiation of ultra-violet light and acquisition of fluorescence and a multichannel optical photodiode-array spectrometer. Autofluorescence spectra evoked by pulsed laser ultra-violet light (wavelength:363nm) were measured from the rat brain, and their changes induced by brain ischemia were observed. Two broad bands with peaks at 400nm and 460nm were observed in the autofluorescence spectra of the brain. The fluorescence with a peak at 460nm increased during brain ischemia induced by occlusion of the bilateral common carotid arteries under hypotension, while the peak at 400nm remained unchanged. The augmantation of autofluorescence during brain ischemia may be due to an increase in NAD(P)H under the hypoxic condition. These results suggest that the fine fiberscope systems for fluorescence observation will be applicable to clinical monitoring system for tissue oxygenation of the epidural and subarachnoid spaces.
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