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Effects of mechanical forces on the development and growth of cartilage

Research Project

Project/Area Number 02557071
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

SUZUKI Fujio  Faculty of Dentistry Osaka University Professor, 歯学部, 教授 (40028717)

Co-Investigator(Kenkyū-buntansha) INOUE Hiroyuki  Faculty of Dentistry Osaka University Instructor, 歯学部, 助手 (90167271)
SAKUDA Mamoru  Faculty of Dentistry Osaka University Professor, 歯学部, 教授 (60028751)
KATO Yukio  Faculty of Dentistry Hiroshima University Professor, 歯学部, 教授 (10112062)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 1991: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1990: ¥5,900,000 (Direct Cost: ¥5,900,000)
KeywordsGrowth-plate Cartilage / Articular Cartilage / Chondrocytes / Mechanical Force / Proliferation / Differentiation / Proteoglycan Synthesis / 軟骨 / 重力 / 力学的影響 / 培養 / 増殖
Research Abstract

Biomechanical forces are thought to have significant effects on the growth of skeletal tissues. However, it is not known how biomechanical forces are translated in these tissues or what changes they cause in the cellular activities. Growth plate and articular cartilage are important as a growth center and a cushion, respectively. Therefore, we examined the effects of centrifugal force on the proliferation of growth-plate and articular chondrocytes and the syntheses of their extracellular matrix in pelleted cultures.
We have shown that pelleted growth-plate chondrocytes in centrifuge tubes can undergo terminal differentiation and then cause calcification of the extracellular matrix, but articular cbondrocytes show scarcely any terminal differentiation asd do not cause calcification. Centrifugation resulted in increased ^3H-thymidine incorporation into DNA in rabbit articular chondrocytes, but had little effect on DNA synthesis in growth-plate chondrocytes in pelleted cultures. Centrifuga … More tion of articular and growth-plate chondrocytes stimulated proteoglycan synthesis. The increase in proteoglycan synthesis by chondrocytes in response to mechanical forces may be necessary for an increase in the strength of cartilaginous tissue, because compressive stiffness of cartilages to mechanical forces has beerv shown to be associated with an increase in the concentration of glycosaminoglycans. The mechanism by which centrifugation stimulates proteoglycan synthesis by chondrocytes is not known. However, in pelleted rabbit chondrocyte cultures, indomethacin did not modulate the effect of centrifugation on ^<35>S-sulf'ate incorporation into proteoglycans. Therefore, it is not likely that prostaglandins are involved in these effect by centrifugal force. Because chondrocytes produce insulinlike growth factors (IGFs), transforming growth factor-beta, and chondromodulins, these factors may play a role in mediating the effect of mechanical forces. Our results provide evidence that mechanical forces act directly on chondrocytes to modulate their proliferation and extracellular matrix synthesis. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] H.Inoue,K.Hiasa,Y.Samma,O.Nakamura,M.Sakuda,M.Iwamoto,F.Suzuki & Y.Kato: "Stimulation of proteoplycan and DNA syntheses in chondrocytes by centrifugation" J.Dent.Res.69. 1560-1563 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Koike,M.Iwamoto,A.Shimazu,K.Nakashima,F.Suzuki & Y.Kato: "Potent mitogenic effects of parathyroid hormone (PTH) on embryonic chick and rabbit chondrocytes:Differential effects of age on growth,proteoglycan,and cyclic AMP responses of chondrocyts to PTH" J.Clin.Invest.85. 626-631 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Sato,M.Iwamoto,K.Nakashima,F.Suzuki & Y.Kato: "1-alpha,25-Dihydroxyvitamin D_3 stimulates colony formation of chick enbryo chondrocytes in soft agar" Exp.Cell Res.187. 335-338 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Kato,A.Shimazu,M.Iwamoto,K.Nakashima,T.Koike,F.Suzuki,Y.Nishii & K.Sato: "Role of 1,25-dihydroxycholecalciferol in growth-plate cartilage:inhibition of terminal differentiation of chondrocytes in vitro and in vivo" Proc.Natl.Acad.Sci.USA. 87. 6522-6526 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Iwamoto,A.Shimazu,K.Nakashima,F.Suzuki & Y.Kato: "Reduction in basic fibroblast growth factor receptor is coupled with terminal differentiation of chondrocytes" J.Biol.Chem.266. 461-467 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Hiraki,H.Tanaka,H.Inoue,J.Kondo,A.Kamizono & F.Suzuki: "Molecular cloning of a new class of cartilage-specific matrix,chondromodulin-I,which stimulates growth of cultured chondrocytes" Biochem.Biophys.Res.Commun.175. 971-977 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] F.Suzuki,M.Takigawa,T.Takano-Yamamoto,Y.Hiraki,N.Endo,Y.Kato,M.Silbermann,M.Iwamoto & A.Asada: "Mechanobiological Research on the Masticatory System(Cytochemical and biochemical approach to masticatory system)" VEB Verlage fur Medizin und Biologie Berlin in GDR, 250 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Inoue,J.Kato,M.Sakuda,M.Iwamoto,F.Suzuki & Y.Kato: "Fundamentals of Bone Growth:Methodology and Applications(A.D.Dixon,B.G.Sarnat & D.A.N.Hoyte,eds.)" CRG Press,Boca,Raton,Florida,USA, 590 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] F. Suzuki, M. Takigawa, T. Takano-Yamamoto, Y. Hiraki, N. Endo, Y. Kato, M. Silbermann, M. Iwamoto & A. Asada: "Cytochemical and biochemical approach to masticatory system." Mechanbiological Research on the Masticatory System (K. Kubota, ed.), VEB Verlage fur Medizin und Biologie Berlin in GDR. 194-199 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Inoue, K. Hiasa, Y. Samma, O. Nakamura, M. Sakuda, M. Iwamoto, F. Suzuki & Y. Kato: "Stimulation of proteoglycan and DNA syntheses in chondrocytes by centrifugation." J. Dent. Res.69. 1560-1563 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Koike, M. Iwamoto, A. Shimazu, K. Nakashima, F. Suzuki & Y. Kato: "Potent mitogenic effects of parathyroid hormone (PTH) on embryonic chick and rabbit chondrocytes : Differential effects of age on growth, proteoglycan, and cyclic AMP responses of chondrocytes to PTH." J. Clin. Invest.85. 626-631 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Sato, M. Iwamoto, K. Nakashima, F. Suzuki & Y. Kato: "1-alpha, 25-Dihydroxyvitamin D_3 stimulates colony formation of chick embryo chondrocytes in soft agar." Exp. Cell Res.187. 335-338 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Kato, A. Shimazu, M. Iwamoto, K. Nakashima, T. Koike, F. Suzuki, Y. Nishii & K. Sato: "Role of 1, 25-dihydroxycholecalciferol in growth-plate cartilage : Inhibition of terminal differentiation of chondrocytes in vitro and in vivo." Proc. Natl. Acad. Sci. USA. 87. 6522-6526 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Iwamoto, A. Shimazu, K. Nakashima, F. Suzuki & Y. Kato: "Reduction in basic fibroblast growth factor receptor is coupled with terminal differentiation of chondrocytes." J. Biol. Chem.266. 461-467 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Inoue, J. Kato, M. Sakuda, M. Iwamoto, F. Suzuki & Y. Kato: "Effects of mechanical forces on proliferation of rabbit growth-plate and articular chondrocytes and their extracellular matrix synthesis in pelleted cultures." Fundamentals of Bone Growth : Methodology and Applications (A. D. Dixon, B. G. Sarnat & D. A. N. Hoyte, eds.), CRC Press, Boca Raton, Florida, USA. 407-415 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Hiraki, H. Tanaka, H. Inoue, J. Kondo, A. Kamizono & F. Suzuki: "Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-I, which stimulates growth of cultured chondrocytes." Biochem. Biophys. Res. Commun.175. 971-977 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Iwamoto,A.Shimazu,K.Nakashima,F.Suzuki&Y.Kato: "Reduction in basic fibroblast growth factor receptor is coupled with terminal differentiation of chondrocytes" J.Biol.Chem.,. 266. 461-467 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Hiraki,H.Tanaka,H.Inoue,J.Kondo,A.Kamizono&F.Suzuki: "Molecular cooning of a new class of cartilage-specific matrix,chondromodurin-I,Which stimulates growth of cultured chondrocytes" Biochem.Biophys.Commun. 175. 971-977 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Inoue,J.Kato,M.Sakuda,M.Iwamoto,F.Suzuki&Y.Kato: "Fundamentals of Bone Growth:Methodology and Applications(A.D.Dixon,B.G.Sarnat,&D.A.N.Hoyte,eds.)" CRC Press,Boca,Raton,Florida,USA, 407-415 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tatsuya Koike et al: "Potent Mitogenic Effects of Parathyroid Hormone(PTH) on Embryonic Chick and Rabbit Chondrocytes" J.Clin.Invest.85. 626-631 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Katsuhiko Sato et al: "1α,25ーDihydroxyvitamin D_3 Stimulates Colony Formation of Chick Embryo Chondrocytes in soft Agar" Exp.Cell Res.187. 335-338 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukio Kato et al: "Effects of Parathyroid Hormore and Calcification on Alkaline Phosphatase Activity and Matrix Calcification in Rabbit GrowthーPlate Chondrocyte Cultures" Endocrinology. 127. 114-118 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiroyuki Inoue et al: "Stimulation of Proteoglycan and DNA synthesis in Chondrocytes by Centrifugation" J.Dental Res.69. 1560-1563 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukio Kato et al.: "Fibroblast growth factor is an inhibitor of chondrocyte terminal differentiation" J.Biol.Chem.265. 5903-5909 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Weigun Yan et al.: "Stimulation by Con conavalin A of Cartilage matrix proteoglycan synthesis in chondrocyte cultures" J.Biol.Chem.265. 10125-10131 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukio Kato et al: "Role of 1,25ーdihydroxycholecalciferol in growthーplate cartilage:in hibition of terminal differentiation of chondrocytes in vitro and in vivo." Proc.Natl.Acad.Su.U.S.A. 87. 6522-6526 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masahiro Iwamoto et al: "Reduction in basic fibroblast growth factor receptor is coupled with terminal differentiation of chondrocytes." J.Biol.Chem.266. 461-467 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 中島 和久 ら: "血小板に存在する軟骨細胞増殖因子iの精製(日本骨代謝学会奨励賞)" 日本骨代謝学会誌. 8. 255-261 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 島津 篤 ら: "軟骨細胞培養系における副甲状腺ホルモンによるインスリン様成長因子の産生促進" 日本骨代謝学会誌. 9. 37-42 (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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