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The development of anti-glomerular nephritis drug

Research Project

Project/Area Number 02557091
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MUROTA Sei-itsu  Tokyo Med.&Dent.Univ., Department of Physiological Chemistry, Graduate School, Professor, 歯学部, 教授 (50072989)

Co-Investigator(Kenkyū-buntansha) IWANO Masao  Mitsubishi Kasei Corp., Research Development Divis Sinior Researcher, 医薬開発部, 部員
IKEDA Junichi  Tokyo Med.&Dent.Univ., Department of Physiological Chemistry, Graduate School, A, 歯学部, 助手 (60222882)
ISHIZAKI Yasuki  Tokyo Med.&Dent.Univ., Department of Physiological Chemistry, Graduate School, A, 歯学部, 助手 (90183003)
MORITA Ikuo  Tokyo Med.&Dent.Univ., Department of Physiological Chemistry, Graduate School, A, 歯学部, 助教授 (60100129)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 1992: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1991: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1990: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsneuron / glutamic acid / lipid peroxide / glutathione / neuronal cell death / 神経細胞障害
Research Abstract

The injury of endothelial cells and neuronal cells is deeply involved in the initiation of atherogenesis and senile dementia, respectively. Therefore, it is important to know what the mechanism of the cell injury is, and how to protect these cells from the injury. We found the HPETEs (hydroperoxyeicosatetraenoic acid) had a potent capacity to cause severe cell injury. During the cours of search for cytoprotective drugs, we found MCI-186 having a remarkable cytoprotective effect on the endothelial cell injury due to HPETEs. 3x10^<-5> M 15-HPETE caused cell death by 60%. But the cytotoxicity was almost completely abolished by the treatment of endothelial cells with 10^<-5> M MCI-186. On the other hand, effects of 15-HPETE on cultured nueronal cells prepared from hippocampi of 19-day-old rat embryos were examines. When neurons were exposure, however, disintegration of both cell bodies and neueites was apparent, which was preceded in many cases by swelling of the soma. Reduced glutathione … More effectively prevented the neuronal injury by 15-HPETE.Ebselen, apparently prevented the swelling of the soma, but was not effective in preventing the disintegration of the neurites.
Neurons which contain nicotinamide adenine dinucleotide phosphate diaphorase(NADPH-d) are know to be spared in several neurological disease in which glutamate neurotoxicity is hypothesized to be involved. Since glutamate neurotoxicity has been largely attributed to an excess loading of calcium ion in neuronal cytoplasm([Ca^<2+>]), we evaluated the changes in [Ca^<2+>] of NADPH-d positive cells after glutamate exposure, In light of recent evidence that neuronal NADPH-d is a nitric oxide synthase(NOS), we futjer evaluated the effect of inhibitor of NOS on glutamate induced calcium influx.
We found 8 NADPH-d positive neurons in 63 experiment (about 3000 neurons). In 4 NADPH-d positive neurons evaluated without L-NMMA, rise in [Ca^<2+>] induced by glutamate revealed to be less than that of LNMMA, however, there was no apparent difference in the time cours of [Ca^<2+>] between NADPH-d positive neurons and 4 negative cells.
Our results indicate that nitric oxide may play an important role in modulating calcium influx through glutamate recepter, providing a new possible therapeutic approach for glutamate neurotoxicity. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] L.MA,Y.ISHIZAKI I.MORITA and S.MUROTA: "Presence of nitric synthase activity in the neyrons of the rat embryonal cerebrum" NEUROSCI.IETTERS,. 132. 23-25 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.ISHIZAKI and S.MUROTA: "Arachidonic acid metabolism in cultured astrocytes,Presence of 12-lipoxygenase activity in the intact cells" NEUROSCI.LETTERS,. 131. 149-152 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI A.MORI,T.SASAKI K.TAKAKURA and S.MUROTA: "The role of cerebral microvessel endothelium in regulation of cerebral blood flow through production of endothelin-1" J.CARDIOVAS.PHARMAC.,. 17. S259-262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.YOSHIMOTO,Y.ISHIZAKI T.SASAKI and S.MUROTA: "Effect of carbon dioxide and oxygen on endothelian production by cultured cerebral endothelial cells." STROKE,. 22. 378-383 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.ISHIZAKI,L.MA,I.MORITA and S.MUROTA: "Astrocytes are responsive to endothelium-derived relaxing factor (EDRF)." NEUROSCI.LETTERS,. 125. 29-30 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.IKEDA,I.MORITA,S.MUROTA,F.SEKIGUCHI,and T.MIYATAKE: "Cerebellar nitric oxide synthase activity is reduced in neurins and Purkinje cell degeneration mutants but not in climbing fiver-lesioned mice" NEUROSCI.LETTERS,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] I.MORITA,H.OCHI and S.MUROTA: "OXIDATIVE DAMAGE AND REPAIR CHEMICAL,BIOLOGY AND MEDICAL ASPECTS,(K.J.DAVIES,ed)" Pergamon Press,New York., 899 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S,MUROTA,H,FUJITA and I.MORITA: "INTRACTABLE VASCULITIS SYNDROME,(T,TANABE,ed.)" Hokkaido Univ.Press,Sapporo,284 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] L.MA,Y.ISHIZAKI,I.MORITA and S.MUROTA: "Presence of nitric synthase activity in the neurons of the rat embryonal cerebrum" 132. 23-25 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.ISHIZAKI and S.MUROTA: "Arachidonic acid metabolism in cultured astrocytes ; presence of 12-lipoxygenase activity in the intact cells" NEUROSCI.LETTERS.131. 149-152 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI,A.MORI,T.SASAKI,T.TAKAKURO and S.MUROTA: "The role of cerebral microvessel endothelium in regulation of cebral blood flow through production of endothelin-1" J.CARDIOVAS.PHARMAC.S256-262 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.YOSHIMOTO,Y.ISHIZAKI,T.SASAKI and S.MUROTA: "Effect of carbon dioxide and oxygen on endothelium production by cultured cerebral endothelial cells" STROKE. 22. 378-383 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.ISHIZAKI,L.MA,I.MORITA and S.MUROTA: "Astrocytes are responsive to endothelium-dervied relaxing factor (EDRF)" NEUROSCI.LETTERS.29-30 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.IKEDA,I.MORITA,S.MUROTA,F.SEKIGUCHI, and T.MIYATAKE: "Cerebellar nitric oxide synthase activity is reduced in neurons and purkinje cell degeneration mutants but not in climbing fiver-lesioned mice" NEUROSCI.LETTERS.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] I.MORITA,H.OCHI and S.MUROTA: OXIDATIVE DAMAGE AND REPAIR CHEMICAL, BIOLOGY AND MEDICAL ASPECTS, (K.J.DAVIES, ed). Pergamon press, New York, 899 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.MUROTA,H.FUJITA and I.MORITA: INTRACTABLE VASCULITIS SYNDROM, (T.TANABE, ed). Hokkaido Univ.Press, Sapporo, 284 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] L.MA,Y.ISHIZAKI I.MORITA and S.MUROTA: "Presence of nitric synthase activity in the neyrons of the rat embryonal cerebrum" NEUROSCI.LETTERS,. 132. 23-25 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.ISHIZAKI and S.MUROTA: "Arachidonic acid metabolism in cultrued astrocytes;presence of 12-lipoxygenase activity in the intact cells" NEUROSCI.LETTERS,. 131. 149-152 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI A.MORI,T.SASAKI K.TAKAKURA and S.MUROTA: "The role of cerebral microvessel endothelium in regulat-ion of cerebral blood flow through production of endothelin-1" J.CARDIOVAS.PHARMAC.,. 17. S259-262 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.YOSHIMOTO,Y.ISHIZAKI T.SASAKI and S.MUROTA: "Effect of carbon dioxide and oxygen on endothelian production by cultured cerebral endothelial cells" STROKE,. 22. 378-383 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.ISHIZAKI,L.MA,I.MORITA and S.MUROTA: "Astrocytes are responsive to endothelium-derived relax-ing factor(EDRF)." NEUROSCI.LETTERS,. 125. 29-30 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] M.IKEDA,I.MORITA,S.MUROTA,F.SEKIGUCHI,and T.MIYATAKE: "Cerebellar nitric oxide synthase activity is reduced in neurins and Purkinje cell degeneration mutants but not in climbing fiver-lesioned mice" NEUROSCI.LETTERS,.

    • Related Report
      1992 Annual Research Report
  • [Publications] I.MORITA,H.OCHI and S.MUROTA: "OXIDATIVE DAMAGE AND REPAIR CHEMICAL,BIOLOGY AND MEDICAL ASPECTS,(K.J.DAVIES,ed)" Pergamon press,New York., 899 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] S,MUROTA,H,FUJITA and I.MORITA: "INTERACTABLE VASCULITIS SYNDROME,(T.TANABE,ed.)" Hokkaido Univ.Press,Sapporp,284 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI,T.SASAKI and S.MUROTA: "Effect of carbon dioxide and oxygen on endothelin production by cultured cerebral endothelial cells" STROKE.22. 378-383 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI,A.MORI,T.SASAKI,K.TAKAKURA and S.MUROTA: "The role of cerebral microvessel endothelium in regulation of cerebral blood flow through production of endothelin-1" J.CARDIOVAS.PHARMACOL.17. S259-262 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.ISHIZAKI,L.MA,I.MORITA and S.MUROTA: "Astrocytes are responsive to endothelium-derived relaxing factor(EDRF)" NEUROSCI.LETTERS,. 125. 29-30 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.FUJITA,I.MORITA,and S.MUROTA: "Involvement of adhesion molecules (CD11a-ICAM-1) in vascular endothelial cell injury elicited by PMA-stimulated neutrophils" BIOCHEM.BIOPHYS.RES.COMMUM.,. 177. 664-672 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] L.MA,Y.ISHZAKI,I.MORITA and S.MUROTA: "Presence of nitric oxide synthase activity in the neurons of the rat embryonal cerebrum" NEUROSCI.LETTERS,. 132. 23-25 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.ISHIZAKI and S.MUROTA: "Arachidonic acid metabolism in cultured astrocytes:presence of 12-1ipoxygenase activity in the intact cells" NEUROSCI.LETTERS,. 131. 149-152 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.OCHI,I.MORITA and S.MUROTA: "Protective effect of several drugs on endothelial cell injury induced by 15-HPETE,an arachidonate lipoxygenase product" CYTOPROTECTION AND CYTOBIOLOGY,(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] I.MORITA,H.OCHI and S.MUROTA: "Cytoprotective effects of glutathione and glutathione peroxidase on endothelial cell injiry induced by lipid peroxydation" Proceedings of International Society for Free Radical Research 5th Biennial Meeting,(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI,H.KURIHARA,T.SASAKI,M.YOSHIZUMI,M.YANAGISAWA,Y.YAZAKI,T.MASAKI and S.MUROTA: "Cerebral microvessel endothelium is producing endothelin" BRAIN RES.508. 283-285 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.ISHIZAKI,T.KANAYASU,and S.MUROTA: "A migration stimulating factor for vascular endothelial cells is released by cultured astrocytes" PROC.JPN.ACAD.66. 81-83 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.KAWASHIMA,N.WATANABE,H.OOHATA,K.FUJIMOTO,T.SUZUKI,Y.ISHIZAKI,I.MORITA and S.MUROTA: "Synthesis and release of acetylcholine by cultured bovine aortic endothelial cells" NEUROSCI.LETTERS.119. 156-158 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.YOSHIMOTO,Y.ISHIZAKI,T.SASAKI and S.MUROTA: "Effect of carbon dioxide and oxygen on endothelin production by cultured cerebral endothelial cells" STROKE.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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