| Project/Area Number |
02557092
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Okayama University |
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Principal Investigator |
HAYATSU Hikoya Okayama University, Faculty of Pharmaceutical Sciences Professor, 薬学部, 教授 (10012593)
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| Co-Investigator(Kenkyū-buntansha) |
HASEBE Kaoru Daiwa Engineering Co, Research Center Director, 所長
KIRA Shohei Okayama University School of Medicine Lecturer, 医学部, 講師 (50033212)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 1991: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1990: ¥6,400,000 (Direct Cost: ¥6,400,000)
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| Keywords | Blue rayon / Mutagenicity / River water / Environmental mutagens / Benzo(a)pyrene / ブル-レ-ヨン / 環境水域 / 水域汚染 / 海水 |
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| Research Abstract |
In this project, we intended to establish a new method for monitoring mutagenicity of environmental waters and have accomplished the intention. Blue rayon is an adsorbents relective for compounds having polycyclic structures with planar surface. This material was invented in 1983 by Hayatsu, the principal investigator of this project, and is a rayon product bearing copper phthalocyanine trisulfonate as ligand. In this project, we hung blue rayon in river waters for 24 hrs, collected the blue rayon, eluted the polycyclic substances that had been adsorbed to the rayon, and the eluted substances were subjected to the mutagenicity assay on Salmonella. We carried out field work with this technique, in Yodo River, Osaka-Kyoto and in the Seto Inland Sea. This work showed that (1) Yodo River was polluted with mutagens of high potency which were then characterized to be polycyclic aromatic amines; and (2) benzo(a)pyrene was detectable at multiple coast sites of the Seto Inland Sea.
With these results, we concluded that although semiquantitative, this new technique is a simple and efficient means to monitor contamination of environmental waters with polycyclic mutagenic compounds.
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